Establishing stable and highly osteogenic hiPSC-derived MSCs for 3D-printed bone graft through microenvironment modulation by CHIR99021-treated osteocytes DOI Creative Commons

Qiuling Guo,

Jingjing Chen,

Qiqi Bu

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101111 - 101111

Published: June 1, 2024

Human induced pluripotent stem cell (hiPSC)-derived mesenchymal cells (iMSCs) are ideal candidates for the production of standardised and scalable bioengineered bone grafts. However, stable induction osteogenic differentiation iMSCs pose challenges in industry. We developed a precise method to produce homogeneous fully differentiated iMSCs. In this study, we established system prepare with increased potential improved bioactivity by introducing CHIR99021 (C91)-treated microenvironment (COOME). COOME enhances mineralisation via canonical Wnt signalling. Global transcriptome analysis co-culturing experiments indicated that pro-angiogenesis/neurogenesis activity The superior abilities COOME-treated were also confirmed Bio3D module generated using polycaprolactone (PCL) cell-integrated 3D printing (PCI3D) system, which is closest model vivo research. This offers new perspective generating highly osteogenic, bioactive, anatomically matched grafts clinical applications. Although human cell-derived MSCs seed synthetic implants, hinder their application. study preparation combining our iMSC osteocyte (COOME) through activation Moreover, upregulated pro-angiogenic pro-neurogenic capacities iMSCs, crucial integration implanted ability was modules PCL systems, highlighting functional vivo. contributes tissue engineering providing insights into regeneration.

Language: Английский

Nanomedicine in cancer therapy DOI Creative Commons

Dahua Fan,

Yongkai Cao,

Meiqun Cao

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Aug. 7, 2023

Cancer remains a highly lethal disease in the world. Currently, either conventional cancer therapies or modern immunotherapies are non-tumor-targeted therapeutic approaches that cannot accurately distinguish malignant cells from healthy ones, giving rise to multiple undesired side effects. Recent advances nanotechnology, accompanied by our growing understanding of biology and nano-bio interactions, have led development series nanocarriers, which aim improve efficacy while reducing off-target toxicity encapsulated anticancer agents through tumor tissue-, cell-, organelle-specific targeting. However, vast majority nanocarriers do not possess hierarchical targeting capability, their indices often compromised poor accumulation, inefficient cellular internalization, inaccurate subcellular localization. This Review outlines current prospective strategies design organelle-targeted nanomedicines, highlights latest progress technologies can dynamically integrate these three different stages static maximize outcomes. Finally, we briefly discuss challenges future opportunities for clinical translation nanomedicines.

Language: Английский

Citations

313

Chemiluminescent probes in cancer biology DOI
Rachel Blau, Omri Shelef, Doron Shabat

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(9), P. 648 - 664

Published: June 19, 2023

Language: Английский

Citations

48

Efficient Delivery of Lomitapide using Hybrid Membrane‐Coated Tetrahedral DNA Nanostructures for Glioblastoma Therapy DOI
Mingming Song,

Jiameng Tian,

Wang Li

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(24)

Published: April 3, 2024

Abstract Glioblastoma (GBM) is the most aggressive and prevalent primary malignant tumor of central nervous system. Traditional chemotherapy has poor therapeutic effects significant side due to drug resistance, natural blood‐brain barrier (BBB), nonspecific distribution, leading a lack clinically effective drugs. Here, 1430 small molecule compounds are screened based on high‐throughput screening platform novel anti‐GBM drug, lomitapide (LMP) obtained. Furthermore, bionic nanodrug delivery system (RFA NPs) actively targeting GBM constructed, which mainly consists tetrahedral DNA nanocages (tFNA loaded with LMP as core folate‐modified erythrocyte–cancer cell–macrophage hybrid membrane (FRUR) shell. FRUR camouflage conferred unique features tFNA NPs, including excellent biocompatibility, improved pharmacokinetic profile, efficient BBB permeability, ability. The results show that RFA NPs exhibited superior specific activities, reduced off‐target delivery, prolonged lifespan, negligible in tumor‐bearing mice. This study combines biomimetic technology provide theoretical practical basis for development optimization clinical treatment strategies treatment.

Language: Английский

Citations

20

3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators DOI Creative Commons
Sarah M. Hull, Junzhe Lou,

Christopher Lindsay

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(13)

Published: March 31, 2023

Three-dimensional bioprinting has emerged as a promising tool for spatially patterning cells to fabricate models of human tissue. Here, we present an engineered bioink material designed have viscoelastic mechanical behavior, similar that living This is cross-linked through dynamic covalent bonds, reversible bond type allows cellular remodeling over time. Viscoelastic materials are challenging use inks, one must tune the kinetics cross-links allow both extrudability and long-term stability. We overcome this challenge small molecule catalysts competitors temporarily modulate cross-linking degree network formation. These inks were then used print model breast cancer cell invasion, where inclusion was found be required formation invasive protrusions. Together, demonstrate power engineered, bioinks recapitulate native microenvironment disease modeling.

Language: Английский

Citations

43

3D bioprinting strategy for engineering vascularized tissue models DOI Open Access
Suhun Chae, Dongheon Ha, Hyungseok Lee

et al.

International Journal of Bioprinting, Journal Year: 2023, Volume and Issue: 9(5), P. 748 - 748

Published: May 9, 2023

Leveraging three-dimensional (3D) bioprinting in the fields of tissue engineering and regenerative medicine has rapidly accelerated progress toward development living constructs biomedical devices. Ongoing vigorous research pursued 3D vitro models to replicate key aspects human physiology by incorporating relevant cell populations adequate environmental cues. Given their advantages being able intimately mimic heterogeneity complexity native counterparts, hold promise as alternatives conventional cultures or animal for translational application model physiology/pathology drug screening. Research highlighted importance models, a sophisticated biomanufacturing strategy is vitally required. In particular, vascularization critical prolonged survival functional maturation engineered tissues, which remained one major challenges establishment physiologically models. To this end, can efficiently generate solid reproducible vascularized with high architectural compositional similarity leading improve structural tissue-specific functionality. Multiple strategies have been developed vascularize tissues spatially controlled patterning vascular precursors generating readily perfusable structures. This review presents an overview advanced development. We present elements rebuilding vasculature 3D-bioprinted discuss recent achievements using bioprinting. Finally, we delineate current future outlooks bioprinting-based

Language: Английский

Citations

26

Micro-engineering and nano-engineering approaches to investigate tumour ecosystems DOI
Mijin Kim, Magdalini Panagiotakopoulou, Chen Chen

et al.

Nature reviews. Cancer, Journal Year: 2023, Volume and Issue: 23(9), P. 581 - 599

Published: June 23, 2023

Language: Английский

Citations

25

Organoid bioinks: construction and application DOI Creative Commons

Fuxiao Wang,

Peiran Song,

Jian Wang

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(3), P. 032006 - 032006

Published: May 2, 2024

Abstract Organoids have emerged as crucial platforms in tissue engineering and regenerative medicine but confront challenges faithfully mimicking native structures functions. Bioprinting technologies offer a significant advancement, especially when combined with organoid bioinks-engineered formulations designed to encapsulate both the architectural functional elements of specific tissues. This review provides rigorous, focused examination evolution impact bioprinting. It emphasizes role bioinks that integrate key cellular components microenvironmental cues more accurately replicate complexity. Furthermore, this anticipates transformative landscape invigorated by integration artificial intelligence bioprinting techniques. Such fusion promises refine bioink optimize parameters, thus catalyzing unprecedented advancements medicine. In summary, accentuates pivotal potential advancing therapies, deepening our understanding organ development, clarifying disease mechanisms.

Language: Английский

Citations

13

Cancer Metastasis‐on‐a‐Chip for Modeling Metastatic Cascade and Drug Screening DOI Creative Commons
Anastasia Brooks, Yali Zhang, Jiezhong Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: Jan. 15, 2024

Microfluidic chips are valuable tools for studying intricate cellular and cell-microenvironment interactions. Traditional in vitro cancer models lack accuracy mimicking the complexities of vivo tumor microenvironment. However, cancer-metastasis-on-a-chip (CMoC) combine advantages 3D cultures microfluidic technology, serving as powerful platforms exploring mechanisms facilitating drug screening. These able to compartmentalize metastatic cascade, deepening understanding its underlying mechanisms. This article provides an overview current CMoC models, focusing on distinctive that simulate invasion, intravasation, circulation, extravasation, colonization, their applications Furthermore, challenges faced by technologies discussed, while promising future directions research. The ongoing development integration these into studies expected drive transformative advancements field.

Language: Английский

Citations

11

Recent advances in 3D bioprinting for cancer research: From precision models to personalized therapies DOI

Ruchika,

Neha Bhardwaj, Sudesh Kumar Yadav

et al.

Drug Discovery Today, Journal Year: 2024, Volume and Issue: 29(4), P. 103924 - 103924

Published: Feb. 22, 2024

Language: Английский

Citations

11

Combating cisplatin-resistant lung cancer using a coiled-coil lipopeptides modified membrane fused drug delivery system DOI
Xi Wang,

Guiquan Liu,

Xueyu Pu

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 45 - 58

Published: Jan. 8, 2025

Language: Английский

Citations

2