A Large Model-Derived Algorithm for Complex Organoids with Internal Morphogenesis and Digital Marker Derivation DOI

Hanghang Liu,

Haohan Xu,

Yu Zhu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19258 - 19266

Published: Oct. 24, 2024

Automated segmentation and evaluation algorithms have been demonstrated to enhance the simplicity translational utility of organoid technology. However, there is a pressing need for development complex organoids that possess epithelium environmental elements, dense regional cell aggregation, intraorganoid morphologies. Nevertheless, has limited progress, including both construction data sets algorithms, in use user-friendly microscopy address such organoids. In this study, set bright-field living fluorescence images paired forms with temporal variance was constructed using droplet-engineered lung Additionally, large model-based algorithm developed. Both contours morphologies were included set, their physical parameters screened form multiplex digital markers evaluation. The outperform existing methods therefore suitable It expected will facilitate successful demonstration AI decision-making regarding status.

Language: Английский

Folic acid-modified ginger-derived extracellular vesicles for targeted treatment of rheumatoid arthritis by remodeling immune microenvironment via the PI3K-AKT pathway DOI Creative Commons

Ruina Han,

Dongyang Zhou,

Ning Ji

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 23, 2025

Rheumatoid arthritis (RA), a form of autoimmune inflammation, is marked by enduring synovial inflammation and the subsequent impairment joint function. Despite availability conventional treatments, they are often marred significant side effects associated high costs. Plant-derived extracellular vesicles (PEVs) offer compelling alternative, owing to their abundant availability, affordability, low immunogenicity, biocompatibility, feasibility for large-scale production. These enhance intercellular communication transferring intrinsic bioactive molecules. In our research, we delve into capacity PEVs treat RA, highlighting role ginger-derived (GDEVs). By conjugating GDEVs with folic acid (FA), have developed FA-GDEVs that maintain inherent immunomodulatory properties. designed selectively target M1 macrophages in inflamed joints via folate receptors (FRs). Our vitro findings indicate promote polarization towards reparative M2 macrophage phenotype modulating PI3K-AKT pathway. Further corroboration comes from vivo studies, which demonstrate not only concentrate efficiently affected but also markedly reduce manifestations RA. Synthesizing these findings, it evident emerge as hopeful candidate RA treatment, offering benefits such safety, therapeutic efficacy.

Language: Английский

Citations

3
Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing DOI Creative Commons
Zuhao Li,

Jinlong Liu,

Jian Song

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217

Published: Jan. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Language: Английский

Citations

14
Engineered hydrogel microspheres for spheroids and organoids construction DOI

Tingting Gai,

Yuanwei Zhang, Guangfeng Li

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 155131 - 155131

Published: Aug. 24, 2024

Language: Английский

Citations

14
3-D bioprinted human-derived skin organoids accelerate full-thickness skin defects repair DOI Creative Commons
Tao Zhang, Shihao Sheng,

Weihuang Cai

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 257 - 269

Published: Sept. 4, 2024

Language: Английский

Citations

9
Advances, challenges and future applications of liver organoids in experimental regenerative medicine DOI Creative Commons

Da Gong,

Jian Mo,

Mei Zhi Zhai

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 24, 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including metabolism of drugs and nutrients. After damage, can rapidly return to its original size if causative factor promptly eliminated. However, when harmful stimulus persists, liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at levels gene expression, molecular interactions, intercellular dynamics, complemented by successful animal studies. robust model carrier that closely resemble physiology are still lacking translating these theories into practice. potential regeneration central focus ongoing research. Over past decade, advent organoid technology provided improved models materials advancing research efforts. Liver represents novel vitro culture system. several years refinement, organoids now accurately replicate morphological structure, nutrient drug metabolism, secretory functions, providing disease Regenerative medicine aims or tissue functions repair replace damaged tissues, restore their structure function, stimulate tissues organs within body. possess same function as tissue, offering serve viable replacement liver, aligning with goals medicine. This review examines role

Language: Английский

Citations

1
Mechano‐Responsive Biomaterials for Bone Organoid Construction DOI Creative Commons
Ruiyang Li, Jian Wang,

Qiushui Lin

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract Mechanical force is essential for bone development, homeostasis, and fracture healing. In the past few decades, various biomaterials have been developed to provide mechanical signals that mimic natural microenvironment, thereby promoting regeneration. Bone organoids, emerging as a novel research approach, are 3D micro‐bone tissues possess ability self‐renew self‐organize, exhibiting biomimetic spatial characteristics. Incorporating mechano‐responsive in construction of organoids presents promising avenue simulating microenvironment. Therefore, this review commences by elucidating impact on health, encompassing both cellular interactions alterations structure. Furthermore, most recent applications within realm tissue engineering highlighted. Three different types introduced with focus their responsive mechanisms, strategies, efficacy facilitating Based comprehensive overview, prospective utilization future challenges discussed. As organoid technology advances, these poised become powerful tools

Language: Английский

Citations

4
A review of 3D bioprinting for organoids DOI Creative Commons

Zeqing Li,

Long Chen, Jialin Wu

et al.

Medical Review, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in physiological environment. Three-dimensional (3D) organoids hold immense potential mimicking key functions human organs overcoming traditional 2D models. However, current techniques preparation 3D had reproducibility, scalability, and ability to closely replicate complex microenvironment found vivo . Additionally, culture systems often involve lengthy labor-intensive processes that hinder high-throughput applications necessary a large-scale screening. Advancements bioprinting technologies offer promising solutions these challenges enabling precise spatial control over placement material composition, thereby facilitating creation more physiologically relevant than techniques. This review provides comprehensive summary recent advances creating models, which begins with an introduction different types (especially focus on volumetric (VBP) technique), followed overview bioinks utilized bioprinting. Moreover, we also introduce disease efficiency evaluation regenerative medicine. Finally, possible strategies development clinical translation are concluded.

Language: Английский

Citations

0
Bioinks for engineering gradient-based osteochondral and meniscal tissue substitutes: a review DOI

Mahdieh Heydarigoojani,

Maryam Farokhi,

Sara Simorgh

et al.

Biofabrication, Journal Year: 2025, Volume and Issue: 17(2), P. 022005 - 022005

Published: Jan. 31, 2025

Gradient tissues are anisotropic structure with gradual transition in structural and biological properties. The gradient structural, mechanical biochemical properties of osteochondral meniscal play a major role defining tissue functions. Designing substitutes that replicate these is crucial to facilitate regeneration functions following injuries. Advanced manufacturing technologies such as 3D bioprinting hold great potentials for recreating nature through using zone-specific bioinks layer-by-layer deposition spatially defined biomaterials, cell types bioactive cues. This review highlighted the gradients detail, elaborated on individual components bioink, reviewed recent advancements gradient-based substitutes. Finally, key challenges field future perspectives developing were discussed. insights from advances can broaden possibilities engineering tissues.

Language: Английский

Citations

0
The 3C (Cell Culture, Computer Simulation, Clinical Trial) Solution for Optimizing the 3R (Replace, Reduction, Refine) Framework during Preclinical Research Involving Laboratory Animals DOI
Sounak Roy,

Mubeena Parveen,

Asis Bala

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Advances in smart biomaterials that modulate the bone microenvironment to promote bone defect repair in diabetes mellitus DOI Creative Commons
Ruideng Wang, Xi He,

Shilong Su

et al.

Smart Materials in Medicine, Journal Year: 2024, Volume and Issue: 5(3), P. 359 - 372

Published: July 31, 2024

Diabetes mellitus (DM) is a chronic metabolic disorder that can affect the balance of bone metabolism and microenvironment, leading to impaired fracture healing. There are several underlying mechanisms which contributing diabetic microenvironment such as hyperglycemia, production advanced glycation end products (AGEs), inflammation, oxidative stress, etc. Recent studies have achieved great progress in developing novel smart biomaterials improving promote In this paper, we reviewed on DM-induced Meanwhile, also summarized used improve local fractures healing, provides perspective for future treatment patients.

Language: Английский

Citations

3