Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(41), P. 14385 - 14393
Published: Oct. 7, 2022
Enzymes
featuring
high
catalytic
efficiency
and
selectivity
have
been
widely
used
as
the
sensing
element
in
analytical
chemistry.
However,
structural
fragility
poor
machinability
of
an
enzyme
significantly
limit
its
practicability
biosensors.
Herein,
we
develop
a
robust
sensitive
hybrid
biosensor
by
means
co-encapsulating
enzymes
into
defective
metal–organic
framework
(MOF),
followed
double-crosslinked
alginate
gelatinization.
The
MOF
encapsulation
can
enhance
stability
enzymes,
yet
well
preserve
their
biocatalytic
function,
while
gelatinization
allows
biohybrid
stretchability
mechanical
strength,
which
facilitates
integration
bead-,
fiber-,
sheet-like
portable
biosensor.
In
this
work,
consisting
glucose
oxidase
peroxidase
are
co-encapsulated
hydrogel,
it
efficiently
convert
blue-violet
product
through
cascade
encapsulated
enabling
colorimetric
biosensing
on
miniaturized
hydrogel
when
coupling
with
smartphone.
Interestingly,
outputs
stronger
signal
than
free
powders,
attributed
to
product-accumulated
effect
highly
hydrophilic
microenvironment
hydrogel.
As
result,
sensitively
selectively
sense
linear
range
from
0.05
4
mM.
Importantly,
both
"armor"
endow
durability,
activity
was
well-maintained
even
after
placing
at
room
temperature
for
30
d.
We
believe
that
has
huge
potential
engineering
next-generation
Chemical Society Reviews,
Journal Year:
2022,
Volume and Issue:
51(15), P. 6251 - 6290
Published: Jan. 1, 2022
Enzyme
immobilization
has
been
developing
since
the
1960s
and
although
many
industrial
biocatalytic
processes
use
technology
to
improve
enzyme
performance,
still
today
we
are
far
from
full
exploitation
of
field.
One
clear
reason
is
that
evaluate
based
on
only
a
few
experiments
not
always
well-designed.
In
contrast
other
reviews
subject,
here
highlight
pitfalls
using
incorrectly
designed
protocols
explain
why
in
cases
sub-optimal
results
obtained.
We
also
describe
solutions
overcome
these
challenges
come
conclusion
recent
developments
material
science,
bioprocess
engineering
protein
science
continue
open
new
opportunities
for
future.
this
way,
immobilization,
being
mature
discipline,
remains
as
subject
high
interest
where
intense
research
necessary
take
advantage
possibilities.
Green Chemistry,
Journal Year:
2021,
Volume and Issue:
23(14), P. 4980 - 5005
Published: Jan. 1, 2021
The
entrapment
of
enzymes
in
organic,
inorganic
or
biomaterials
can
yield
active
immobilized
biocatalysts
for
chemical
transformations
without
the
need
to
form
covalent
bonds
with
protein.
Chemical Reviews,
Journal Year:
2021,
Volume and Issue:
122(1), P. 1052 - 1126
Published: Nov. 30, 2021
Biocatalysis,
using
enzymes
for
organic
synthesis,
has
emerged
as
powerful
tool
the
synthesis
of
active
pharmaceutical
ingredients
(APIs).
The
first
industrial
biocatalytic
processes
launched
in
half
last
century
exploited
whole-cell
microorganisms
where
specific
enzyme
at
work
was
not
known.
In
meantime,
novel
molecular
biology
methods,
such
efficient
gene
sequencing
and
triggered
breakthroughs
directed
evolution
rapid
development
process-stable
with
broad
substrate
scope
good
selectivities
tailored
substrates.
To
date,
are
employed
to
enable
shorter,
more
efficient,
sustainable
alternative
routes
toward
(established)
small
molecule
APIs,
additionally
used
perform
standard
reactions
API
efficiently.
Herein,
large-scale
synthetic
containing
key
steps
>130
APIs
approved
drugs
drug
candidates
compared
corresponding
chemical
protocols
(if
available)
regarding
steps,
reaction
conditions,
scale.
review
is
structured
according
functional
group
formed
reaction.
ChemSusChem,
Journal Year:
2022,
Volume and Issue:
15(9)
Published: Jan. 13, 2022
In
the
movement
to
decarbonize
our
economy
and
move
away
from
fossil
fuels
we
will
need
harness
waste
products
of
activities,
such
as
lignocellulose,
methane,
carbon
dioxide.
Our
wastes
be
integrated
into
a
circular
where
used
are
recycled
manufacturing
cycle.
Key
this
recycling
plastics
at
resin
monomer
levels.
Biotechnology
is
well
suited
future
chemical
industry
that
must
adapt
widely
distributed
diverse
biological
feedstocks.
increasing
mastery
biotechnology
allowing
us
develop
enzymes
organisms
can
synthesize
widening
selection
desirable
bulk
chemicals,
including
plastics,
commercially
viable
productivities.
Integration
bioreactors
with
electrochemical
systems
permit
new
production
opportunities
enhanced
productivities
advantage
using
low-carbon
electricity
renewable
sustainable
sources.
ChemSusChem,
Journal Year:
2022,
Volume and Issue:
15(9)
Published: March 3, 2022
The
role
and
power
of
biocatalysis
in
sustainable
chemistry
has
been
continuously
brought
forward
step
by
to
its
present
outstanding
position.
problem-solving
capabilities
have
realized
numerous
substantial
achievements
biology,
engineering.
Advances
breakthroughs
the
life
sciences
interdisciplinary
cooperation
with
clearly
accelerated
implementation
biocatalytic
synthesis
modern
chemistry.
Resource-efficient
manufacturing
processes
already
provided
benefits
as
well
customer-centric
value
creation
pharmaceutical,
food,
flavor,
fragrance,
vitamin,
agrochemical,
polymer,
specialty,
fine
chemical
industries.
Biocatalysis
can
make
significant
contributions
not
only
processes,
but
also
design
completely
new
value-creation
chains.
now
be
considered
a
key
enabling
technology
implement
Processes,
Journal Year:
2022,
Volume and Issue:
10(3), P. 494 - 494
Published: March 1, 2022
Enzymes
are
outstanding
(bio)catalysts,
not
solely
on
account
of
their
ability
to
increase
reaction
rates
by
up
several
orders
magnitude
but
also
for
the
high
degree
substrate
specificity,
regiospecificity
and
stereospecificity.
The
use
development
enzymes
as
robust
biocatalysts
is
one
main
challenges
in
biotechnology.
However,
despite
specificities
turnover
enzymes,
there
drawbacks.
At
industrial
level,
these
drawbacks
typically
overcome
resorting
immobilized
enhance
stability.
Immobilization
allows
reuse,
increases
stability,
facilitates
process
control,
eases
product
recovery,
enhances
yield
quality.
This
especially
important
expensive
those
obtained
low
fermentation
with
relatively
activity.
review
provides
an
integrated
perspective
(multi)enzyme
immobilization
that
abridges
a
critical
evaluation
methods
carriers,
biocatalyst
metrics,
impact
key
carrier
features
performance,
trends
towards
miniaturization
detailed
illustrative
examples
representative
biocatalytic
applications
promoting
sustainability.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(16)
Published: Jan. 17, 2022
Enzymes
incorporated
into
hydrogen-bonded
organic
frameworks
(HOFs)
via
bottom-up
synthesis
are
promising
biocomposites
for
applications
in
catalysis
and
sensing.
Here,
we
explored
synthetic
incorporation
of
d-amino
acid
oxidase
(DAAO)
with
the
metal-free
tetraamidine/tetracarboxylate-based
BioHOF-1
water.
N-terminal
enzyme
fusion
positively
charged
module
Z
