Identifying Pyroptosis-Hub Genes and Inflammation Cell Type-Related Genes in Ischemic Stroke DOI Creative Commons

Wei Ge,

Liangbin Cao,

Can Liu

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Stroke is the second-leading global cause of death. The damage attributed to immune storm triggered by ischemia–reperfusion injury (IRI) post-stroke substantial. However, data on transcriptomic dynamics pyroptosis in IRI are limited. This study aimed analyze expression key genes stroke and their correlation with infiltration. Pyroptosis-related were identified from obtained middle cerebral artery occlusion (MCAO) datasets. Differential functional analyses pyroptosis-related performed, differences enrichment between high-risk low-risk groups determined. An MCAO diagnostic model was constructed validated using selected differential expression. High- for cell analysis hub genes. A regulatory network miRNA also constructed, protein domains predicted. an rat model. Twenty-five showed expression, including four genes, namely WISP2, MELK, SDF2L1, AURKB. Characteristic verified real-time quantitative PCR analyses. high- significant SDF2L1. In infiltration analysis, 12 cells samples. Further demonstrated positive correlations gene SDF2L1 cell-activated dendritic group natural killer group. elevated closely associated inflammatory types can predict ischemic risk levels help facilitate treatment.

Language: Английский

In vivo dendritic cell reprogramming for cancer immunotherapy DOI
Ervin Ascic, Fritiof Åkerström, M. Nair

et al.

Science, Journal Year: 2024, Volume and Issue: 386(6719)

Published: Sept. 5, 2024

Immunotherapy can lead to long-term survival for some cancer patients, yet generalized success has been hampered by insufficient antigen presentation and exclusion of immunogenic cells from the tumor microenvironment. Here, we developed an approach reprogram in vivo adenoviral delivery transcription factors PU.1, IRF8, BATF3, which enabled them present antigens as type 1 conventional dendritic cells. Reprogrammed remodeled their microenvironment, recruited, expanded polyclonal cytotoxic T cells; induced regressions; established systemic immunity multiple mouse melanoma models. In human spheroids xenografts, reprogramming dendritic-like progressed independently immunosuppression, usually limits immunotherapy. Our study paves way clinical trials immune cell

Language: Английский

Citations

20
Cross-priming in cancer immunology and immunotherapy DOI
Carlos Luri‐Rey, Álvaro Teijeira, Stefanie K. Wculek

et al.

Nature reviews. Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

Citations

3
The regulatory role of integrin in gastric cancer tumor microenvironment and drug resistance DOI
Songlin Wu, Abdel Nasser B. Singab,

Guimei LlN

et al.

Progress in Biophysics and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Anti-TGF-β/PD-L1 bispecific antibody synergizes with radiotherapy to enhance antitumor immunity and mitigate radiation-induced pulmonary fibrosis DOI Creative Commons
Yuze Wu,

Yuheng Yan,

Yarong Guo

et al.

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 5, 2025

Despite the success of immune checkpoint inhibitors (ICIs) in multiple malignant tumors, a significant proportion patients remain unresponsive to treatment. Radiotherapy (RT) elicits immunogenic antitumor responses but concurrently activates several evasion mechanisms. Our earlier research demonstrated efficacy YM101, an anti-TGF-β/PD-L1 bispecific antibody, stroma-rich tumors. Nevertheless, YM101 has reduced effectiveness non-inflamed tumors characterized by poor cell infiltration. This study investigated potential synergy between RT and overcoming immunotherapy resistance mitigating RT-induced pulmonary fibrosis. The activity survival outcomes plus treatment vivo were explored murine tumor models. Furthermore, inhibition metastases was assessed metastasis model. impact on dendritic (DC) maturation quantified flow cytometry, whereas cytokine chemokine secretions measured ELISA. To comprehensively characterize changes microenvironment, we utilized combination methods, including IHC staining, multiplex inmunofluorecence RNA sequencing. Additionally, evaluated significantly inhibited growth, prolonged compared with monotherapies promoted DC dose-dependent manner increased proinflammatory cytokines. Mechanistically, simultaneously infiltration activation intratumoral DCs tumor-infiltrating lymphocytes reshaped microenvironment landscape. Notably, attenuated both peritumoral fibrosis findings suggest that combined enhances immunity overcomes preclinical models, while showing therapy demonstrates promise ICI resistance, potentially sparing normal tissue, thereby providing strong rationale for further clinical investigations.

Language: Английский

Citations

2
Targeting novel regulated cell death: Ferroptosis, pyroptosis and necroptosis in anti‐PD‐1/PD‐L1 cancer immunotherapy DOI Creative Commons
Li Yu, Ke Huang, Yixiang Liao

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(8)

Published: April 9, 2024

Abstract Chemotherapy, radiotherapy, and immunotherapy represent key tumour treatment strategies. Notably, immune checkpoint inhibitors (ICIs), particularly anti‐programmed cell death 1 (PD1) ligand (PD‐L1), have shown clinical efficacy in immunotherapy. However, the limited effectiveness of ICIs is evident due to many cancers exhibiting poor responses this treatment. An emerging avenue involves triggering non‐apoptotic regulated (RCD), a significant mechanism driving cancer diverse treatments. Recent research demonstrates that combining RCD inducers with significantly enhances their antitumor across various types. The use anti‐PD‐1/PD‐L1 activates CD8 + T cells, prompting initiation novel forms, such as ferroptosis, pyroptosis, necroptosis. functions mechanisms anti‐PD1/PD‐L1 therapy remain insufficiently explored. This review summarises roles necroptosis It emphasises synergy between nanomaterials PD‐1/PD‐L1 induce different Furthermore, targeting signalling pathways combination therapies holds promise prospective strategy for

Language: Английский

Citations

13
Stimuli-Responsive mRNA Vaccines to Induce Robust CD8+ T Cell Response via ROS-Mediated Innate Immunity Boosting DOI

Linying Dong,

Xuqian Deng,

Yan Li

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(28), P. 19218 - 19228

Published: July 2, 2024

The messenger RNA (mRNA) vaccines hold great significance in contagion prevention and cancer immunotherapy. However, safely effectively harnessing innate immunity to stimulate robust durable adaptive immune protection is crucial, yet challenging. In this study, we synthesized a library of stimuli-responsive bivalent ionizable lipids (srBiv iLPs) with smart molecular blocks responsive esterase, H

Language: Английский

Citations

9
Tetrandrine activates STING/TBK1/IRF3 pathway to potentiate anti-PD-1 immunotherapy efficacy in non-small cell lung cancer DOI Creative Commons
Yan Zhi Tan,

Qiancheng Zhu,

Meilin Yang

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 207, P. 107314 - 107314

Published: July 24, 2024

The efficacy of PD-1 therapy in non-small cell lung cancer (NSCLC) patients remains unsatisfactory. Activating the STING pathway is a promising strategy to improve inhibitor efficacy. Here, we found tetrandrine (TET), an anti-tumor compound extracted from medicinal plant commonly used traditional Chinese medicine, has ability inhibit NSCLC tumor growth. Mechanistically, TET induces nuclear DNA damage and increases cytosolic dsDNA, thereby activating STING/TBK1/IRF3 pathway, which turn promotes infiltration dendritic cells (DCs), macrophages, as well CD8+ T mice. In vivo imaging dynamically monitored increased activity after treatment predicted activation immune microenvironment. We further revealed that combination with αPD-1 monoclonal antibody (αPD-1 mAb) yields significant anti-cancer effects by promoting enhancing its cell-killing effect, reduced growth tumors prolonged survival Therefore, effectively eliminates enhances immunotherapy through combining anti-PD-1 deserves exploration for applications.

Language: Английский

Citations

9
Dendritic cell subsets and implications for cancer immunotherapy DOI Creative Commons
Michael Y. Chen, Felicia Zhang, S. Peter Goedegebuure

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 5, 2024

Dendritic cells (DCs) play a central role in the orchestration of effective T cell responses against tumors. However, their functional behavior is context-dependent. DC type, transcriptional program, location, intratumoral factors, and inflammatory milieu all impact DCs with regard to promoting or inhibiting tumor immunity. The following review introduces important facets function, how subset phenotype can affect interplay other factors microenvironment. It will also discuss current cancer treatment relies on survey myriad ways which immune therapy more directly harness enact antitumor cytotoxicity.

Language: Английский

Citations

8
CXCL14 in prostate cancer: complex interactions in the tumor microenvironment and future prospects DOI Creative Commons
Lei Tang, Xin Chen, Jianquan Hou

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 4, 2025

CXCL14 is a highly conserved chemokine expressed in various cell types, playing crucial roles both physiological and pathological processes, including immune regulation tumorigenesis. Recently, the role of tumors has attracted considerable attention. However, previous pan-cancer studies have reported inconsistencies regarding effects on tumors, particularly concerning its expression levels tumor tissues influence phenotypes cancer cells. This variability believed to stem from context-dependent nature CXCL14, as different sources secretion within distinct microenvironments may mediate diverse biological effects. Such phenomena also been observed prostate research. Despite foundational understanding cancer, there remains lack comprehensive reviews summarizing specific this systematically analyzing reasons behind complex Therefore, article aims discuss microenvironment explore future research directions potential applications.

Language: Английский

Citations

1
Interplay between extracellular vesicles and allergic diseases: Current insights and future perspectives DOI Creative Commons
Jia Wu, Yan Wang, Yuqing Wang

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract The field of extracellular vesicle (EV) research has rapidly evolved, revealing their significant roles in various biological processes and clinical applications. Allergic diseases are prevalent immunological disorders characterized by exaggerated hypersensitivity reactions to allergens, leading diverse manifestations that can severely impact quality life. Recently, studies have underscored the pivotal role EVs allergic conditions, including asthma, rhinitis, atopic dermatitis. In this review, we provide a comprehensive overview recent advances separation characterization EVs, diseases, diagnostic value as biomarkers. Additionally, explore therapeutic potential for treatment prevention conditions. Overall, emerging insights into present promising opportunities enhancing diagnosis, treatment, management diseases.

Language: Английский

Citations

1