Merged Molecular Switches Excel as Optoacoustic Dyes: Azobenzene–Cyanines Are Loud and Photostable NIR Imaging Agents DOI Creative Commons
Markus Müller, Nian Liu, Vipul Gujrati

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: May 29, 2024

Abstract Optoacoustic (or photoacoustic) imaging promises micron‐resolution noninvasive bioimaging with much deeper penetration (>cm) than fluorescence. However, optoacoustic of enzyme activity would require loud, photostable, NIR‐absorbing molecular contrast agents, which remain unknown. Most organic agents are repurposed fluorophores, severe shortcomings photoinstability or phototoxicity under imaging, as consequences their slow S 1 →S 0 electronic relaxation. We now report that known fluorophores can be rationally modified to reach ultrafast rates, without extra complexity, simply by merging them switches. Here, we merge azobenzene switches cyanine dyes give relaxation (<10 ps, >100‐fold faster). Without even adapting instrument settings, these azohemicyanines display outstanding improvements in signal longevity (>1000‐fold increase photostability) and loudness (>3‐fold at time zero). show why this simple but unexplored design strategy still offer stronger performance the future, also spatial resolution quantitative linearity photoacoustic response over extended longitudinal imaging. By bringing world rotors bear on problems facing practical will help unleash full potential fundamental studies translational uses.

Language: Английский

Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges DOI
Wonseok Choi, Byullee Park, Seongwook Choi

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(11), P. 7379 - 7419

Published: Jan. 16, 2023

For decades now, photoacoustic imaging (PAI) has been investigated to realize its potential as a niche biomedical modality. Despite highly desirable optical contrast and ultrasonic spatiotemporal resolution, PAI is challenged by such physical limitations low signal-to-noise ratio (SNR), diminished image due strong attenuation, lower-bound on spatial resolution in deep tissue. In addition, contrast-enhanced faced practical insufficient cell-specific targeting delivery efficiency difficulties developing clinically translatable agents. Identifying these essential the continuing expansion of field, substantial advances contrast-enhancing agents, complemented high-performance acquisition systems, have synergistically dealt with challenges conventional PAI. This review covers past four years research pushing terms SNR/contrast, targeted delivery, clinical application. Promising strategies for dealing each challenge are reviewed detail, future directions next generation discussed.

Language: Английский

Citations

125
Biomarker-activatable probes based on smart AIEgens for fluorescence and optoacoustic imaging DOI
Juan Ouyang, Lihe Sun, Fang Zeng

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 458, P. 214438 - 214438

Published: Feb. 3, 2022

Language: Английский

Citations

90
Label-Free Optical Metabolic Imaging in Cells and Tissues DOI Creative Commons
Irene Georgakoudi, Kyle P. Quinn

Annual Review of Biomedical Engineering, Journal Year: 2023, Volume and Issue: 25(1), P. 413 - 443

Published: April 27, 2023

Over the last half century, autofluorescence of metabolic cofactors NADH (reduced nicotinamide adenine dinucleotide) and FAD (flavin has been quantified in a variety cell types disease states. With spread nonlinear optical microscopy techniques biomedical research, imaging offered an attractive solution to noninvasively monitor tissue status elucidate dynamic changes or metabolism. Various tools methods measure temporal, spectral, spatial properties have developed. Specifically, redox ratio cofactor fluorescence intensities lifetime parameters used numerous applications, but significant work remains mature this technology for understanding This article describes current our sensitivity different pathways highlights challenges field. Recent progress addressing these acquiring more quantitative information faster metabolically relevant formats is also discussed.

Language: Английский

Citations

56
Clinical translation of photoacoustic imaging DOI
Jeongwoo Park, Seongwook Choi, Ferdinand Knieling

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Language: Английский

Citations

28
Simultaneous photoacoustic and ultrasound imaging: A review DOI

Yinshi Yu,

Ting Feng, Haixia Qiu

et al.

Ultrasonics, Journal Year: 2024, Volume and Issue: 139, P. 107277 - 107277

Published: Feb. 28, 2024

Language: Английский

Citations

26
Optical diagnostic imaging and therapy for thyroid cancer DOI Creative Commons

Chengying Shao,

Zhenfang Li,

Chengchi Zhang

et al.

Materials Today Bio, Journal Year: 2022, Volume and Issue: 17, P. 100441 - 100441

Published: Sept. 26, 2022

Thyroid cancer, as one of the most common endocrine cancers, has seen a surge in incidence recent years. This is likely due to lack specificity and accuracy its traditional diagnostic modalities, leading overdiagnosis thyroid nodules. Although there are several treatment options available, they limited surgery

Language: Английский

Citations

40
Renal-Clearable Probe with Water Solubility and Photostability for Biomarker-Activatable Detection of Acute Kidney Injuries via NIR-II Fluorescence and Optoacoustic Imaging DOI
Cheng Zeng,

Yunyan Tan,

Lihe Sun

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(14), P. 17664 - 17674

Published: April 3, 2023

Acute kidney injuries (AKI) have serious short-term or long-term complications with high morbidity and mortality rate, thus posing great health threats. Developing high-performance NIR-II probes for noninvasive in situ detection of AKI via fluorescent optoacoustic dual-mode imaging is significance. Yet chromophores often feature long conjugation hydrophobicity, which prevent them from being renal clearable, limiting their applications the diseases. To fully exploit advantageous features heptamethine cyanine dye, while overcoming its relatively poor photostability, to strive design a probe imaging, herein, we developed PEG3-HC-PB, water soluble, biomarker activatable has good photostability. As probe, fluorescence (900-1200 nm) quenched due existence electron-pulling phenylboronic group (responsive element), it exhibits weak absorption peak at 830 nm. Meanwhile, presence overexpressed H2O2 region case AKI, converted phenylhydroxy group, enhances emission (600-900 eventually produces conspicuous signals imaging. This enables contrast-agent-induced ischemia/reperfusion-induced mice using real-time 3D-MSOT response H2O2. Hence, this can be used as practicable tool detecting AKI; additionally, strategy could provide insight into other large-conjugation multifarious biological applications.

Language: Английский

Citations

35
Advances in photoacoustic imaging aided by nano contrast agents: special focus on role of lymphatic system imaging for cancer theranostics DOI Creative Commons
Badrinathan Sridharan, Hae Gyun Lim

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 20, 2023

Photoacoustic imaging (PAI) is a successful clinical platform for management of cancer and other health conditions that has seen significant progress in the past decade. However, translation PAI based methods are still under scrutiny as quality information derived from PA images not on par with methods. Hence, to improve PAI, exogenous contrast agents, form nanomaterials, being used achieve better image less side effects, lower accumulation, improved target specificity. Nanomedicine become inevitable management, it contributes at every stage diagnosis therapy, surgery, even postoperative care surveillance recurrence. Nanocontrast agents have been developed explored early diagnosis. The systemic stability specificity nanomaterials render its theranostic property depends various influencing factors such administration route physico-chemical responsiveness. recent focus targeting lymphatic system nodes diagnosis, they play vital role progression metastasis. This review aims discuss advancements using nanoparticles theranostics emphasis progression, metastasis, guided tumor resection, finally drug delivery.

Language: Английский

Citations

31
A multifunctional nanoaggregate‐based system for detection of rheumatoid arthritis via Optoacoustic/NIR‐II fluorescent imaging and therapy via inhibiting JAK‐STAT/NF‐κB/NLRP3 pathways DOI Creative Commons
Junjie Chen, Longqi Chen,

Zunpan She

et al.

Aggregate, Journal Year: 2023, Volume and Issue: 5(1)

Published: Sept. 11, 2023

Abstract Rheumatoid arthritis (RA) is a debilitating autoimmune disease that causes chronic pain and serious complications, presenting significant challenge to treat. Promising approaches for treating RA involve signaling pathways modulation targeted therapy. To this end, multifunctional nanosystem, TPC‐U@HAT, has been designed therapy, featuring multitargeting, dual‐stimuli response, on‐demand drug release capabilities. TPC‐U@HAT composed of probe/prodrug TPC, JAK1 kinase inhibitor upadacitinib, the carrier HAT. TPC an aggregation‐induced emission (AIE)‐active NIR‐II chromophore TPY NF‐κB/NLRP3 caffeic acid phenethyl ester (CAPE), connected via boronic bond which serves as reactive‐oxygen‐species‐responsive linker. The carrier, HAT, created by grafting bone‐targeting alendronate hydrophobic tocopheryl succinate onto hyaluronic chains, can encapsulate upadacitinib form TPC‐U@HAT. Upon intravenous injection into mice, accumulates at inflamed lesions through both active passive targeting, overexpressed hyaluronidase H 2 O therein cleave polymer chains boronate bonds, respectively. This generates AIE‐active detection therapeutic evaluation optoacoustic imaging fluorescent concomitantly releases CAPE exert efficacious therapy inhibiting JAK‐STAT pathways.

Language: Английский

Citations

30
Illuminating the Brain: Advances and Perspectives in Optoelectronics for Neural Activity Monitoring and Modulation DOI Creative Commons
Shumao Xu,

Marzia Momin,

Salahuddin Ahmed

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(42)

Published: Sept. 19, 2023

Abstract Optogenetic modulation of brain neural activity that combines optical and electrical modes in a unitary system has recently gained robust momentum. Controlling illumination spatial coverage, designing light‐activated modulators, developing wireless light delivery data transmission are crucial for maximizing the use neuromodulation. To this end, biocompatible electrodes with enhanced optoelectrical performance, device integration multiplexed addressing, transmission, multimodal operation soft systems have been developed. This review provides an outlook uniformly illuminating large areas while spatiotemporally imaging responses upon stimulation little artifacts. Representative concepts important breakthroughs, such as head‐mounted illumination, multiple implanted fibers, micro‐light‐delivery devices, discussed. Examples techniques incorporate electrophysiological monitoring presented. Challenges perspectives posed further research efforts toward high‐density interface modulation, potential nonpharmacological neurological disease treatments stimulation.

Language: Английский

Citations

28