The maternal-to-zygotic transition revisited

Development, Journal Year: 2019, Volume and Issue: 146(11)

Published: June 1, 2019

The development of animal embryos is initially directed by maternal gene products. Then, during the maternal-to-zygotic transition (MZT), developmental control handed to zygotic genome. Extensive research in both vertebrate and invertebrate model organisms has revealed that MZT can be subdivided into two phases, which very different modes regulation are implemented: initially, exclusively post-transcriptional post-translational, following gradual activation genome leads predominance transcriptional regulation. These changes expression program precisely controlled highly interconnected. Here, we review current understanding mechanisms underlie handover MZT.

Language: Английский

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Insights into epigenetic patterns in mammalian early embryos

Protein & Cell, Journal Year: 2020, Volume and Issue: 12(1), P. 7 - 28

Published: July 15, 2020

Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to formation a totipotent embryo. During development pre-implantation embryo, precise reprogramming progress is prerequisite for avoiding developmental defects or embryonic lethality, but underlying molecular mechanisms remain elusive. For past few years, unprecedented breakthroughs have been made in mapping regulatory network dynamic epigenomes during mammalian early embryo development, taking advantage multiple advances and innovations low-input genome-wide chromatin analysis technologies. The aim this review highlight most recent understanding embryogenesis mammals, including DNA methylation, histone modifications, accessibility 3D organization.

Language: Английский

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Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early Drosophila development

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(4), P. 477 - 486

Published: April 1, 2021

Language: Английский

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Increased somatic mutation burdens in normal human cells due to defective DNA polymerases

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(10), P. 1434 - 1442

Published: Sept. 30, 2021

Abstract Mutation accumulation in somatic cells contributes to cancer development and is proposed as a cause of aging. DNA polymerases Pol ε δ replicate during cell division. However, some cancers, defective proofreading due acquired POLE / POLD1 exonuclease domain mutations causes markedly elevated mutation burdens with distinctive mutational signatures. Germline familial predisposition. Here, we sequenced normal tissue tumor from individuals germline mutations. Increased characteristic signatures were found adult types, early embryogenesis sperm. Thus human physiology can tolerate ubiquitously burdens. Except for increased risk, do not exhibit overt features premature These results support model which all aging are attributable widespread malfunction directly resulting accrued life.

Language: Английский

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Lineage tracing of human development through somatic mutations

Nature, Journal Year: 2021, Volume and Issue: 595(7865), P. 85 - 90

Published: May 12, 2021

Language: Английский

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GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo

eLife, Journal Year: 2021, Volume and Issue: 10

Published: March 15, 2021

Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal-to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA Factor (GAF). GAF necessary activate widespread remodel accessibility landscape. demonstrated that preferentially controls expression earliest transcribed genes, while genes expressed during activation predominantly dependent on GAF. Thus, progression MZT requires coordination multiple pioneer-like factors, we propose proceeds control gradually transferred from

Language: Английский

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Clonal dynamics in early human embryogenesis inferred from somatic mutation

Nature, Journal Year: 2021, Volume and Issue: 597(7876), P. 393 - 397

Published: Aug. 25, 2021

Language: Английский

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Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(3), P. 400 - 418.e13

Published: Feb. 9, 2022

Language: Английский

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Aneuploidy in mammalian oocytes and the impact of maternal ageing

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 24(1), P. 27 - 44

Published: Sept. 6, 2022

Language: Английский

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Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition

Science Advances, Journal Year: 2022, Volume and Issue: 8(5)

Published: Feb. 2, 2022

Translational regulation plays an important role in gene expression and function. Although the transcriptional dynamics of mouse preimplantation embryos have been well characterized, global mRNA translation landscape master regulators zygotic genome activation (ZGA) remain unknown. Here, by developing applying a low-input ribosome profiling (LiRibo-seq) technique, we profiled revealed translational during development. We identified marked transition from MII oocytes to zygotes demonstrated that active maternal mRNAs is essential for maternal-to-zygotic (MZT). further showed two factors, Smarcd2 Cyclin T2, whose activated zygotes, are required chromatin reprogramming ZGA, respectively. Our study thus not only filled knowledge gap on mammalian development but also insights into critical function MZT.

Language: Английский

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