Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes DOI Creative Commons
Rose Bulteau,

Lucie Barbier,

Guillaume Lamour

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Cell mechanical properties regulate biological processes such as oocyte development. Cortical tension is regulated via actomyosin cortex remodeling to ensure optimal quality. However, the evolution of other parameters and their relationship with structure remain poorly understood in mammalian oocytes. In this work, a methodology combining multiple measured through Atomic Force Microscopy proposed investigate between organization. By studying mouse oocytes at various stages development, along engineered ones specific organization, it demonstrated that thin actin corresponds stiff while thick one associated softer It further revealed maternal age, critical factor for fertility, affects mechanics, correlating alterations structure. Finally, shown differs human highlighting species-specific differences

Language: Английский

Biomarkers of aging DOI Open Access

Hainan Bao,

Jiani Cao, Mengting Chen

et al.

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(5), P. 893 - 1066

Published: April 11, 2023

Language: Английский

Citations

203
Recurrent implantation failure: reality or a statistical mirage? DOI Creative Commons
Paul Pirtea, Marcelle I. Cedars,

Kate Devine

et al.

Fertility and Sterility, Journal Year: 2023, Volume and Issue: 120(1), P. 45 - 59

Published: Feb. 22, 2023

ImportanceTo date, recurrent implantation failure (RIF) has no clear definition and clearly identified impaired function. Hence, the term RIF is currently used somewhat haphazardly, on basis of clinicians’ judgment.ObjectiveInternational experts in reproductive medicine met July 1, 2022, Lugano, Switzerland, to review different facets define diagnosis its appropriate management.Evidence ReviewA systematic without meta-analysis studies published English from January 2015 May 2022.FindingsData indicated that been largely overevaluated, overdiagnosed, overtreated sufficient critical assessment true nature. Our analyses show extremely uncommon—occurring <5% couples with infertility—and reassurance continued conventional therapies are warranted most cases assisted technology (ART) failure. Although biologic determinants may exist a small subset people infertility, they elude available tools for assessment. Without identification underlying etiology(ies), it reasonable not assign this patient until she failed at least 3 euploid blastocyst transfers (or equivalent number unscreened embryo transfers, adjusted patient’s age corresponding euploidy rate). In addition, other factors should be ruled out contribute her reduced odds sustained implantation. such cases, only issue considered case ART because result multiple necessarily repetitive or persistent. reality, impacting probability further success very rare occurrence.ConclusionTrue uncommon, occurring infertility. Reassurance cases. It would seem embryos, age).RelevanceGiven internationally recognized field present Lugano meeting our publication constitutes consensus statement. To judgment. International management. A 2022. Data occurrence. True age).

Language: Английский

Citations

53
Mouse oocytes sequester aggregated proteins in degradative super-organelles DOI Creative Commons
Gabriele Zaffagnini, Shiya Cheng,

Marion Claudia Salzer

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(5), P. 1109 - 1126.e21

Published: Feb. 1, 2024

Oocytes are among the longest-lived cells in body and need to preserve their cytoplasm support proper embryonic development. Protein aggregation is a major threat for intracellular homeostasis long-lived cells. How oocytes cope with protein during extended life unknown. Here, we find that mouse accumulate aggregates specialized compartments named endolysosomal vesicular assemblies (ELVAs). Combining live-cell imaging, electron microscopy, proteomics, found ELVAs non-membrane-bound composed of endolysosomes, autophagosomes, proteasomes held together by matrix formed RUFY1. Functional assays revealed immature oocytes, sequester aggregated proteins, including TDP-43, degrade them upon oocyte maturation. Inhibiting degradative activity leads accumulation embryo detrimental survival. Thus, represent strategy safeguard

Language: Английский

Citations

24
Maternal ELL3 loss-of-function leads to oocyte aneuploidy and early miscarriage DOI

Shiqi Zhu,

Peng Xie, Yi Yang

et al.

Nature Structural & Molecular Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Language: Английский

Citations

2
NADase CD38 is a key determinant of ovarian aging DOI Creative Commons
Qingling Yang, Wenhui Chen, Luping Cong

et al.

Nature Aging, Journal Year: 2023, Volume and Issue: 4(1), P. 110 - 128

Published: Dec. 21, 2023

Abstract The ovary ages earlier than most other tissues, yet the underlying mechanisms remain elusive. Here a comprehensive analysis of transcriptomic landscapes in different organs young and middle-aged mice revealed that ovaries showed expression age-associated genes, identifying increased NADase CD38 decreased NAD + levels mice. Bulk single-cell RNA sequencing deletion mitigated ovarian aging, preserving fertility follicle reserve aged by countering age-related gene changes intercellular communication alterations. Mechanistically, onset inflammation induced higher ovary, thereby accelerating aging. Consistently, pharmacological inhibition enhanced Our findings aging relative to organs, providing potential therapeutic target for ameliorating female infertility.

Language: Английский

Citations

33
Prevalence of chromosomal alterations in first-trimester spontaneous pregnancy loss DOI Creative Commons
Rick Essers, И. Н. Лебедев, Ants Kurg

et al.

Nature Medicine, Journal Year: 2023, Volume and Issue: 29(12), P. 3233 - 3242

Published: Nov. 23, 2023

Pregnancy loss is often caused by chromosomal abnormalities of the conceptus. The prevalence these and allocation (ab)normal cells in embryonic placental lineages during intrauterine development remain elusive. In this study, we analyzed 1,745 spontaneous pregnancy losses found that roughly half (50.4%) products conception (POCs) were karyotypically abnormal, with maternal paternal age independently contributing to increased genomic aberration rate. We applied genome haplarithmisis a subset 94 normal parental POC karyotypes. Genotyping DNA as well extra-embryonic mesoderm chorionic villi DNA, representing trophoblastic tissues, enabled characterization landscape both lineages. Of losses, 35.1% had aberrations not previously detected karyotyping, increasing rate 67.8% extrapolation. contrast viable pregnancies where mosaic are restricted villi, such confined mosaicism, higher degree imbalances rather than villi. Our results stress importance scrutinizing full allelic architecture improve clinical management basic research devastating condition.

Language: Английский

Citations

25
Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging DOI Creative Commons
Qingling Yang, Hui Li, Huan Wang

et al.

Aging Cell, Journal Year: 2023, Volume and Issue: 22(9)

Published: June 18, 2023

Recent advances highlight the pivotal role of nicotinamide adenine dinucleotide (NAD+ ) in ovarian aging. However, roles de novo NAD+ biosynthesis on aging are still unknown. Here, we found that genetic ablation Ido1 (indoleamine-2,3-dioxygenase 1) or Qprt (Quinolinate phosphoribosyl transferase), two critical genes biosynthesis, resulted decreased levels middle-aged mice, leading to subfertility, irregular estrous cycles, reduced reserve, and accelerated Moreover, observed impaired oocyte quality, characterized by increased reactive oxygen species spindle anomalies, which ultimately led fertilization ability early embryonic development. A transcriptomic analysis ovaries both mutant wild-type mice revealed alterations gene expression related mitochondrial metabolism. Our findings were further supported observation distribution membrane potential oocytes knockout mice. Supplementation with riboside (NR), an booster, reserve improved quality. study highlights importance pathway female fertility.

Language: Английский

Citations

24
Hallmarks of ovarian aging DOI

Chuqing Wu,

Dan Chen, Michael B. Stout

et al.

Trends in Endocrinology and Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Ovarian aging: mechanisms and intervention strategies DOI Creative Commons
Zhengmao Zhu, Wanxue Xu, Lin Liu

et al.

Medical Review, Journal Year: 2022, Volume and Issue: 2(6), P. 590 - 610

Published: Nov. 21, 2022

Abstract Ovarian reserve is essential for fertility and influences healthy aging in women. Advanced maternal age correlates with the progressive loss of both quantity quality oocytes. The molecular mechanisms various contributing factors underlying ovarian have been uncovered. In this review, we highlight some critical that impact oocyte during aging. Germ cell follicle at birth determines reproductive lifespan timing menopause female mammals. Accelerated diminishing leads to premature or insufficiency. Poor increasing could result from chromosomal cohesion deterioration misaligned chromosomes, telomere shortening, DNA damage associated genetic mutations, oxidative stress, mitochondrial dysfunction epigenetic alteration. We also discuss intervention strategies delay Both efficacy senotherapies by antioxidants against therapy are discussed. Functional oocytes ovarioids be rejuvenated pluripotent stem cells somatic cells. propose directions future interventions. As couples increasingly begin delaying parenthood life worldwide, understanding potential benefit women making earlier choices about their health.

Language: Английский

Citations

29
Live chromosome identifying and tracking reveals size-based spatial pathway of meiotic errors in oocytes DOI
Osamu Takenouchi, Yogo Sakakibara, Tomoya S. Kitajima

et al.

Science, Journal Year: 2024, Volume and Issue: 385(6706)

Published: July 18, 2024

Meiotic errors of relatively small chromosomes in oocytes result egg aneuploidies that cause miscarriages and congenital diseases. Unlike somatic cells, which preferentially mis-segregate larger chromosomes, aged smaller through unclear processes. Here, we provide a comprehensive three-dimensional chromosome identifying-and-tracking dataset throughout meiosis I live mouse oocytes. This analysis reveals prometaphase pathway actively moves to the inner region metaphase plate. In region, are pulled by stronger bipolar microtubule forces, facilitates premature separation, major segregation study spatial aneuploidy eggs implicates role M phase creating size–based arrangement.

Language: Английский

Citations

7