Nanodiagnostics to Face SARS-CoV-2 and Future Pandemics: From an Idea to the Market and Beyond DOI Creative Commons
Giulio Rosati, Andrea Idili, Claudio Parolo

et al.

ACS Nano, Journal Year: 2021, Volume and Issue: 15(11), P. 17137 - 17149

Published: Oct. 27, 2021

The COVID-19 pandemic made clear how our society requires quickly available tools to address emerging healthcare issues. Diagnostic assays and devices are used every day screen for positive patients, with the aim decide appropriate treatment containment measures. In this context, we would have expected see use of most recent diagnostic technologies worldwide, including advanced ones such as nano-biosensors capable provide faster, more sensitive, cheaper, high-throughput results than standard polymerase chain reaction lateral flow assays. Here discuss why that has not been case all exciting strategies published on a daily basis in peer-reviewed journals yet successful reaching market being implemented clinical practice.

Language: Английский

Wastewater sequencing reveals early cryptic SARS-CoV-2 variant transmission DOI Creative Commons
Smruthi Karthikeyan, Joshua I. Levy,

Peter De Hoff

et al.

Nature, Journal Year: 2022, Volume and Issue: 609(7925), P. 101 - 108

Published: July 7, 2022

Abstract As SARS-CoV-2 continues to spread and evolve, detecting emerging variants early is critical for public health interventions. Inferring lineage prevalence by clinical testing infeasible at scale, especially in areas with limited resources, participation, or and/or sequencing capacity, which can also introduce biases 1–3 . RNA concentration wastewater successfully tracks regional infection dynamics provides less biased abundance estimates than 4,5 Tracking virus genomic sequences would improve community detect variants. However, two factors limit wastewater-based surveillance: low-quality sequence data inability estimate relative mixed samples. Here we resolve these issues perform a high-resolution, 295-day effort, the controlled environment of large university campus broader context surrounding county. We developed deployed improved protocols deconvolution software that fully multiple strains from wastewater. detected concern up 14 days earlier samples, identified instances not captured surveillance. Our study scalable solution surveillance allows detection identification cryptic transmission.

Language: Английский

Citations

379
Use of At-Home COVID-19 Tests — United States, August 23, 2021–March 12, 2022 DOI Open Access
Benjamin Rader, Autumn Gertz, A. Danielle Iuliano

et al.

MMWR Morbidity and Mortality Weekly Report, Journal Year: 2022, Volume and Issue: 71(13), P. 489 - 494

Published: March 25, 2022

COVID-19 testing provides information regarding exposure and transmission risks, guides preventative measures (e.g., if when to start end isolation quarantine), identifies opportunities for appropriate treatments, helps assess disease prevalence (1). At-home rapid antigen tests (at-home tests) are a convenient accessible alternative laboratory-based diagnostic nucleic acid amplification (NAATs) SARS-CoV-2, the virus that causes (2-4). With emergence of SARS-CoV-2 B.1.617.2 (Delta) B.1.1.529 (Omicron) variants in 2021, demand at-home increased

Language: Английский

Citations

199
Recent Progresses in Machine Learning Assisted Raman Spectroscopy DOI Creative Commons
Yaping Qi,

Dan Hu,

Yucheng Jiang

et al.

Advanced Optical Materials, Journal Year: 2023, Volume and Issue: 11(14)

Published: April 26, 2023

Abstract With the development of Raman spectroscopy and expansion its application domains, conventional methods for spectral data analysis have manifested many limitations. Exploring new approaches to facilitate has become an area intensifying focus research. It been demonstrated that machine learning techniques can more efficiently extract valuable information from data, creating unprecedented opportunities analytical science. This paper outlines traditional recently developed statistical are commonly used in (ML) ML‐algorithms different spectroscopy‐based classification recognition applications. The include Principal Component Analysis, K‐Nearest Neighbor, Random Forest, Support Vector Machine, as well neural network‐based deep algorithms such Artificial Neural Networks, Convolutional etc. bulk review is dedicated research advances applied several fields, including material science, biomedical applications, food others, which reached impressive levels accuracy. combination offers achieve high throughput fast identification these fields. limitations current studies also discussed perspectives on future provided.

Language: Английский

Citations

105
Comparison of virus concentration methods and RNA extraction methods for SARS-CoV-2 wastewater surveillance DOI

Xiawan Zheng,

Yu Deng, Xiaoqing Xu

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 824, P. 153687 - 153687

Published: Feb. 5, 2022

Language: Английский

Citations

88
COVID-19 surveillance in wastewater: An epidemiological tool for the monitoring of SARS-CoV-2 DOI Creative Commons
Sajida Maryam, Ihtisham Ul Haq, Galal Yahya

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 5, 2023

The coronavirus disease 2019 (COVID-19) pandemic has prompted a lot of questions globally regarding the range information about virus’s possible routes transmission, diagnostics, and therapeutic tools. Worldwide studies have pointed out importance monitoring early surveillance techniques based on identification viral RNA in wastewater. These indicated presence severe acute respiratory syndrome 2 (SARS-CoV-2) human feces, which is shed via excreta including mucus, saliva, sputum. Subsequently, they get dumped into wastewater, their wastewater provides possibility using it as tool to help prevent eradicate virus. Its still done many regions worldwide serves an “warning signal”; however, limitations also been identified.

Language: Английский

Citations

45
Colorimetric sensing for translational applications: from colorants to mechanisms DOI
Zhicheng Jin, Wonjun Yim, Maurice Retout

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(15), P. 7681 - 7741

Published: Jan. 1, 2024

This review summarizes insights into colorant selection and signal mechanisms for the development of colorimetric sensing POC sensors.

Language: Английский

Citations

42
Localised wastewater SARS-CoV-2 levels linked to COVID-19 cases: A long-term multisite study in England DOI Creative Commons
Natalia R. Jones, Richard Elson, Matthew J. Wade

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 962, P. 178455 - 178455

Published: Jan. 1, 2025

Wastewater-based surveillance (WBS) can monitor for the presence of human health pathogens in population. During COVID-19, WBS was widely used to determine wastewater SARS-CoV-2 RNA concentration (concentrations) providing information on community COVID-19 cases (cases). However, studies examining relationship between concentrations and tend be localised or focussed small-scale institutional settings. Few have examined this multiple settings, over long periods, with large sample numbers, nor attempted quantify detail how catchment characteristics affected these. This 18-month study (07/20-12/21) explored correlation quantitative using censored regression. Our analysis >94,000 samples collected from 452 diverse sampling sites (259 Sewage Treatment Works (STW) 193 Sewer Network Sites (SNS)) covering ~65 % English Wastewater were linked ~6 million diagnostically confirmed cases. High coefficients found (STW: median r = 0.66, IQR: 0.57-0.74; SNS: 0.65, 0.54-0.74). The (regression coefficient) variable catchments. Catchment (e.g. size population grab vs automated sampling) had significant but small effects regression coefficients. last six months reduced became highly coincided a shift towards younger cases, vaccinated rapid emergence variant Omicron. programme rapidly introduced at scale during COVID-19. Laboratory methods evolved catchments characteristics. Despite diversity, findings indicate that provides an effective proxy establishing dynamics across wide variety communities. While there is potential predicting concentration, may more smaller scales.

Language: Английский

Citations

3
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays DOI Creative Commons
Yan Ni, Bas J. H. M. Rosier, Eva A. van Aalen

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: July 28, 2021

Abstract Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with ratiometric bioluminescent readout. The concept entails analyte-induced complementation of split NanoLuc luciferase fragments, photoconjugated to an antibody sandwich pair via protein G adapters. Introduction calibrator provides robust signal allows direct in-sample quantitative measurements complex media blood plasma. We developed sensors allow low-picomolar several biomarkers, anti-drug antibodies, therapeutic both SARS-CoV-2 spike anti-SARS-CoV-2 antibodies. With its easy-to-implement standardized workflow, attractive, fast, low-cost alternative traditional immunoassays, academic setting, clinical laboratories, for applications.

Language: Английский

Citations

78
Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2 DOI
Zahir Ali, Edith Sánchez, Muhammad Tehseen

et al.

ACS Synthetic Biology, Journal Year: 2021, Volume and Issue: 11(1), P. 406 - 419

Published: Dec. 23, 2021

Simple, rapid, specific, and sensitive point-of-care detection methods are needed to contain the spread of SARS-CoV-2. CRISPR/Cas9-based lateral flow assays emerging as a powerful alternative for COVID-19 diagnostics. Here, we developed Bio-SCAN (biotin-coupled specific CRISPR-based assay nucleic acid detection) an accurate pathogen platform that requires no sophisticated equipment or technical expertise. detects SARS-CoV-2 genome in less than 1 h from sample collection result. In first step, target sequence is isothermally amplified 15 min via recombinase polymerase amplification before being precisely detected by biotin-labeled nuclease-dead SpCas9 (dCas9) on commercially available strips. The resulting readout visible naked eye. Compared other CRISPR-Cas-based assays, additional reporters, probes, enhancers, reagents, devices interpret results. highly successfully clinically relevant level (4 copies/μL) synthetic RNA genome. Similarly, showed 100% negative 96% positive predictive agreement with RT-qPCR results when using clinical samples (86 nasopharyngeal swab samples). Furthermore, incorporating variant-specific sgRNAs reaction allowed efficiently distinguish between α, β, δ variants. Also, our confirmed reagents have long shelf life can be assembled locally nonlaboratory limited-resource settings. compatible quick extraction protocol. Our highlight potential promising diagnostic facilitate low-cost mass screening

Language: Английский

Citations

77
Point-of-care diagnostics: recent developments in a pandemic age DOI

Harshit Harpaldas,

Siddarth Arumugam, Chelsey Campillo Rodriguez

et al.

Lab on a Chip, Journal Year: 2021, Volume and Issue: 21(23), P. 4517 - 4548

Published: Jan. 1, 2021

In this review, we provide an overview of developments in point-of-care (POC) diagnostics during the COVID-19 pandemic. We review these advances within framework a holistic POC ecosystem, focusing on points interest - both technological and non-technological to researchers test developers. Technologically, design choices assay chemistry, microfluidics, instrumentation towards nucleic acid protein detection for severe acute respiratory coronavirus 2 (SARS-CoV-2), away from lab bench, that supported unprecedented rapid development, scale up, deployment devices. describe common features technologies obtained Emergency Use Authorization (EUA) acid, antigen, antibody tests, how tests fit into four distinct use cases. conclude with implications future pandemics, infectious disease monitoring, digital health.

Language: Английский

Citations

65