Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(2), P. e3002205 - e3002205

Published: Feb. 1, 2024

Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse eukaryotes, are provided by internal conduits that allow substances travel more readily through tissue than they would via diffusion. Microbes growing in structures, called biofilms, also affected differential we hypothesized is influenced physical arrangement cells. study, examined microanatomy biofilms formed pathogenic bacterium Pseudomonas aeruginosa discovered clonal cells form striations packed lengthwise across most a mature biofilm’s depth. We identified mutants, including those defective pilus function O-antigen attachment, show alterations packing phenotype. Consistent with notion cellular affects within biofilm, found while wild type shows even distribution tested substrates depth, mutants accumulation at biofilm boundaries. Furthermore, altered localization metabolic activity, survival resident cells, susceptibility subpopulations antibiotic treatment. Our observations provide insight into features determine microanatomy, consequences for physiological differentiation drug sensitivity.

Language: Английский

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Application of a quantitative framework to improve the accuracy of a bacterial infection model

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(19)

Published: May 1, 2023

Laboratory models are critical to basic and translational microbiology research. Models serve multiple purposes, from providing tractable systems study cell biology allowing the investigation of inaccessible clinical environmental ecosystems. Although there is a recognized need for improved model systems, gap in rational approaches accomplish this goal. We recently developed framework assessing accuracy microbial by quantifying how closely each gene expressed natural environment various models. The defined as percentage genes that similarly model. Here, we leverage develop validate two generalizable improving accuracy, proof concept, apply these improve Pseudomonas aeruginosa infecting cystic fibrosis (CF) lung. First, identify models, an vitro synthetic CF sputum medium (SCFM2) epithelial model, accurately recapitulate different sets. By combining cell-SCFM2 which improves over 500 genes. Second, specific genes, mined publicly available transcriptome data, identified zinc limitation cue present lung absent SCFM2. Induction SCFM2 resulted accurate expression 90% P. These provide generalizable, quantitative frameworks microbiological improvement can be applied any system interest.

Language: Английский

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Spatial transcriptome uncovers rich coordination of metabolism in E. coli K12 biofilm

Nature Chemical Biology, Journal Year: 2023, Volume and Issue: 19(8), P. 940 - 950

Published: April 13, 2023

Language: Английский

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Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 22, 2023

Abstract Streptococcus mutans has been implicated as the primary pathogen in childhood caries (tooth decay). While role of polymicrobial communities is appreciated, it remains unclear whether other microorganisms are active contributors or interact with pathogens. Here, we integrate multi-omics supragingival biofilm (dental plaque) from 416 preschool-age children (208 males and 208 females) a discovery-validation pipeline to identify disease-relevant inter-species interactions. Sixteen taxa associate metagenomics-metatranscriptomics analyses. Using multiscale/computational imaging virulence assays, examine formation dynamics, spatial arrangement, metabolic activity Selenomonas sputigena, Prevotella salivae Leptotrichia wadei , either individually S. . We show that sputigena flagellated anaerobe previously unknown biofilm, becomes trapped streptococcal exoglucans, loses motility but actively proliferates build honeycomb-like multicellular-superstructure encapsulating enhancing acidogenesis. Rodent model experiments reveal an unrecognized ability colonize tooth surfaces. incapable causing on its own, when co-infected mutans, causes extensive enamel lesions exacerbates disease severity vivo. In summary, discover pathobiont cooperating known unique structure heighten prevalent human disease.

Language: Английский

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Candida–bacterial cross-kingdom interactions

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 31(12), P. 1287 - 1299

Published: Aug. 26, 2023

Language: Английский

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Disentangling the feedback loops driving spatial patterning in microbial communities

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 20, 2025

Language: Английский

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Polymicrobial Interactions of Oral Microbiota: a Historical Review and Current Perspective

mBio, Journal Year: 2022, Volume and Issue: 13(3)

Published: May 2, 2022

The oral microbiota is enormously diverse, with over 700 microbial species identified across individuals that play a vital role in the health of our mouth and overall well-being. In addition, as diseases such caries (cavities) periodontitis (gum disease) are mediated through interspecies interactions, this community serves an important model system to study complexity dynamics polymicrobial interactions.

Language: Английский

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Precise spatial structure impacts antimicrobial susceptibility of S. aureus in polymicrobial wound infections

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(51)

Published: Dec. 15, 2022

A hallmark of microbial ecology is that interactions between members a community shape function. This includes communities in human infections, such as chronic wounds, where can result more severe diseases. Staphylococcus aureus the most common organism isolated from wound infections and has been shown to have both cooperative competitive with Pseudomonas aeruginosa . Still, despite considerable study, these microbes characterized using vitro well-mixed systems, which do not recapitulate infection environment. Here, we S. P. murine focusing on role macro- micro-scale spatial structures play disease. We discovered coexist at high cell densities wounds. High-resolution imaging revealed establish patchy distribution, only occupying 5 25% volume. Using quantitative framework, identified precise structure macro (mm)- micro (µm)-scales, was largely mediated by production antimicrobial 2-heptyl-4-hydroxyquinoline N-oxide, while pyocyanin had no impact. Finally, this enhances tolerance aminoglycoside antibiotics but vancomycin. Our results provide mechanistic insights into biogeography coinfected wounds implicate key determinant infections.

Language: Английский

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Networking between helminths, microbes, and mammals

Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 31(4), P. 464 - 471

Published: April 1, 2023

Just as mammals have coevolved with the intestinal bacterial communities that are part of microbiota, helminths represent an important selective force on their mammalian host. The complex interaction between helminths, microbes, and host is likely determinant mutual fitness. immune system in particular a critical interface both this crosstalk often determines balance tolerance resistance against these widespread parasites. Hence, there many examples how microbiota can influence tissue homeostasis homeostatic immunity. Understanding processes at cellular molecular level exciting area research we seek to highlight review will potentially guide future treatment approaches.

Language: Английский

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Combating Microbial Infections Using Metal-Based Nanoparticles as Potential Therapeutic Alternatives

Antibiotics, Journal Year: 2023, Volume and Issue: 12(5), P. 909 - 909

Published: May 15, 2023

The nature of microorganisms and the efficiency antimicrobials have witnessed a huge co-dependent change in their dynamics over last few decades. On other side, metals metallic compounds gained popularity owing to effectiveness against various microbial strains. A structured search both research review papers was conducted via different electronic databases, such as PubMed, Bentham, Springer, Science Direct, among others, for present review. Along with these, marketed products, patents, Clinicaltrials.gov were also referred our Different microbes bacteria, fungi, etc., diverse species strains been reviewed found be sensitive metal-carrying formulations. products are observed restrict growth, multiplication, biofilm formation effectively adequately. Silver has an apt use this area treatment recovery, like copper, gold, iron, gallium generate antimicrobial activity. identified membrane disruption, oxidative stress, interaction proteins enzymes primary microbicidal processes. Elaborating action, nanoparticles nanosystems shown work favor well excelled rational ways.

Language: Английский

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