Pseudomonas aeruginosa surface motility and invasion into competing communities enhance interspecies antagonism DOI Creative Commons
Andrea Sánchez Peña, James B. Winans, Carey D. Nadell

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: Aug. 6, 2024

ABSTRACT Chronic polymicrobial infections involving Pseudomonas aeruginosa and Staphylococcus aureus are prevalent, difficult to eradicate, associated with poor health outcomes. Therefore, understanding interactions between these pathogens is important inform improved treatment development. We previously demonstrated that P. attracted S. using type IV pili (TFP)-mediated chemotaxis, but the impact of attraction on growth physiology remained unknown. Using live single-cell confocal imaging visualize microcolony structure, spatial organization, survival during coculture, we found interspecies chemotaxis provides a competitive advantage by promoting invasion into disruption microcolonies. This behavior renders susceptible antimicrobials. Conversely, in absence TFP motility, cells exhibit reduced colonies. Instead, builds cellular barrier adjacent secretes diffusible, bacteriostatic antimicrobials like 2-heptyl-4-hydroxyquinoline- N -oxide (HQNO) Reduced leads formation denser thicker colonies increased HQNO-mediated lactic acid fermentation, physiological change could complicate strategies. Finally, show motility modifications structure enhance competition against . Overall, studies expand our how TFP-mediated facilitates interactions, highlighting importance positioning mixed-species communities. IMPORTANCE The nature many chronic makes their eradication challenging. Particularly, coisolation from airways people cystic fibrosis wound common severe clinical complex interplay not fully understood, need for continued research improve management infections. Our study unveils , invades neighboring colonies, anti-staphylococcal factors interior colony. Upon inhibition thus invasion, colony architecture changes dramatically, whereby protected antagonism responds through alterations may further hamper treatment. These reinforce accumulating evidence structuring can dictate community resilience reveal critical drivers competition.

Language: Английский

Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms DOI Creative Commons
Hannah Dayton,

Julie Kiss,

Mian Wei

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(2), P. e3002205 - e3002205

Published: Feb. 1, 2024

Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse eukaryotes, are provided by internal conduits that allow substances travel more readily through tissue than they would via diffusion. Microbes growing in structures, called biofilms, also affected differential we hypothesized is influenced physical arrangement cells. study, examined microanatomy biofilms formed pathogenic bacterium Pseudomonas aeruginosa discovered clonal cells form striations packed lengthwise across most a mature biofilm’s depth. We identified mutants, including those defective pilus function O-antigen attachment, show alterations packing phenotype. Consistent with notion cellular affects within biofilm, found while wild type shows even distribution tested substrates depth, mutants accumulation at biofilm boundaries. Furthermore, altered localization metabolic activity, survival resident cells, susceptibility subpopulations antibiotic treatment. Our observations provide insight into features determine microanatomy, consequences for physiological differentiation drug sensitivity.

Language: Английский

Citations

9
Antibiotic resistance in bacterial communities DOI Creative Commons
Marlis Denk-Lobnig, Kevin B. Wood

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 74, P. 102306 - 102306

Published: April 11, 2023

Language: Английский

Citations

36
Application of a quantitative framework to improve the accuracy of a bacterial infection model DOI Creative Commons
Gina R. Lewin,

Ananya Kapur,

Daniel M. Cornforth

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(19)

Published: May 1, 2023

Laboratory models are critical to basic and translational microbiology research. Models serve multiple purposes, from providing tractable systems study cell biology allowing the investigation of inaccessible clinical environmental ecosystems. Although there is a recognized need for improved model systems, gap in rational approaches accomplish this goal. We recently developed framework assessing accuracy microbial by quantifying how closely each gene expressed natural environment various models. The defined as percentage genes that similarly model. Here, we leverage develop validate two generalizable improving accuracy, proof concept, apply these improve Pseudomonas aeruginosa infecting cystic fibrosis (CF) lung. First, identify models, an vitro synthetic CF sputum medium (SCFM2) epithelial model, accurately recapitulate different sets. By combining cell-SCFM2 which improves over 500 genes. Second, specific genes, mined publicly available transcriptome data, identified zinc limitation cue present lung absent SCFM2. Induction SCFM2 resulted accurate expression 90% P. These provide generalizable, quantitative frameworks microbiological improvement can be applied any system interest.

Language: Английский

Citations

33
Structural characteristics, functions, and counteracting strategies of biofilms in Staphylococcus aureus DOI Creative Commons

Yi-qi Xia,

Zhenghui Hu,

Qiyuan Jin

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: 27, P. 488 - 500

Published: Jan. 1, 2025

Staphylococcus aureus (S. aureus) is a prevalent pathogen associated with wide range of infections, exhibiting significant antibiotic resistance and posing therapeutic challenges in clinical settings. The formation biofilms contributes to the emergence resistant strains, further diminishing efficacy antibiotics. This, turn, leads chronic recurrent ultimately increasing healthcare burden. Consequently, preventing eliminating has become critical focus management research. This review systematically examines mechanisms underlying biofilm S. its contribution resistance, emphasizing essential roles play maintaining structural integrity enhancing resistance. It also analyses protective that fortify against antimicrobial treatments. Furthermore, provides comprehensive overview recent innovations, including enzymatic therapy, nanotechnology, gene editing, phage therapy. Emerging strategies present novel approaches combat biofilm-associated infections through various mechanisms. discusses advancements these therapies, their practical application, an in-depth analysis each strategy's potential. By mapping future research directions, this aims refine anti-biofilm control infection progression effectively mitigate recurrence.

Language: Английский

Citations

1
Analysis of metagenomic data DOI
Shaopeng Liu, Judith Rodríguez, Viorel Munteanu

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
Oral bacteriophages and their potential as adjunctive treatments for periodontitis: a narrative review DOI Creative Commons
Mwila Kabwe, Joseph Tucci, Ivan Darby

et al.

Journal of Oral Microbiology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 25, 2025

Background There is no specific cure for periodontitis and treatment symptomatic, primarily by physical removal of the subgingival plaque biofilm. Current non-surgical periodontal therapy becomes less effective as pocket depth increases such new adjunctive treatments are required. The development antibiotic resistance has driven a recent resurgence interest in bacteriophage therapy.

Language: Английский

Citations

1
Biofilm antimicrobial susceptibility testing: where are we and where could we be going? DOI
Tom Coenye

Clinical Microbiology Reviews, Journal Year: 2023, Volume and Issue: 36(4)

Published: Oct. 9, 2023

SUMMARY Our knowledge about the fundamental aspects of biofilm biology, including mechanisms behind reduced antimicrobial susceptibility biofilms, has increased drastically over last decades. However, this so far not been translated into major changes in clinical practice. While concept is increasingly on radar microbiologists, physicians, and healthcare professionals general, standardized tools to study biofilms microbiology laboratory are still lacking; one area which particularly obvious that testing (AST). It generally accepted lifestyle a tremendous impact antibiotic susceptibility, yet AST typically carried out with planktonic cells. On top that, microenvironment at site infection an important driver for microbial physiology hence susceptibility; but poorly reflected current methods. The goal review provide overview state art concerning highlight gaps area. Subsequently, potential ways improve biofilm-based will be discussed. Finally, bottlenecks currently preventing use practice, as well steps needed get past these bottlenecks,

Language: Английский

Citations

22
Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms DOI Creative Commons
Yuzhen Zhang, Yumin Cai, Bing Zhang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 31, 2024

Biofilm formation enhances bacterial survival and antibiotic tolerance, but the underlying mechanisms are incompletely understood. Here, we show that biofilm growth is accompanied by a reduction in energy metabolism membrane potential, together with metabolic exchanges between inner outer regions biofilms. More specifically, nutrient-starved cells interior supply amino acids to periphery, while peripheral experience decrease potential provide fatty cells. Fatty facilitate repair of starvation-induced damage enhance their presence antibiotics. Thus, contribute tolerance within biofilm. tolerance. authors biofilms interior.

Language: Английский

Citations

8
Natural and synthetic molecules with potential to enhance biofilm formation and virulence properties in Pseudomonas aeruginosa DOI
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum

et al.

Critical Reviews in Microbiology, Journal Year: 2023, Volume and Issue: 50(5), P. 830 - 858

Published: Nov. 15, 2023

can efficiently adapt to changing environmental conditions due its ubiquitous nature, intrinsic/acquired/adaptive resistance mechanisms, high metabolic versatility, and the production of numerous virulence factors. As a result,

Language: Английский

Citations

16
Functional Hydrogels Promote Chronic Infectious Wound Healing by Re-rousing Macrophage M1 and Inducing Bacterial Copper-Like Death DOI Creative Commons
Chaoqun Xiang, Chaoyu Pu,

Xuemei Zhong

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101571 - 101571

Published: Feb. 14, 2025

Language: Английский

Citations

0