Oxidative Stress in Cancer

Cancer Cell, Journal Year: 2020, Volume and Issue: 38(2), P. 167 - 197

Published: July 9, 2020

Language: Английский

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Cholangiocarcinoma 2020: the next horizon in mechanisms and management

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2020, Volume and Issue: 17(9), P. 557 - 588

Published: June 30, 2020

Abstract Cholangiocarcinoma (CCA) includes a cluster of highly heterogeneous biliary malignant tumours that can arise at any point the tree. Their incidence is increasing globally, currently accounting for ~15% all primary liver cancers and ~3% gastrointestinal malignancies. The silent presentation these combined with their aggressive nature refractoriness to chemotherapy contribute alarming mortality, representing ~2% cancer-related deaths worldwide yearly. current diagnosis CCA by non-invasive approaches not accurate enough, histological confirmation necessary. Furthermore, high heterogeneity CCAs genomic, epigenetic molecular levels severely compromises efficacy available therapies. In past decade, efforts have been made understand complexity develop new diagnostic tools therapies might help improve patient outcomes. this expert Consensus Statement, which endorsed European Network Study Cholangiocarcinoma, we aim summarize critically discuss latest advances in CCA, mostly focusing on classification, cells origin, genetic abnormalities, alterations, biomarker discovery treatments. horizon next decade from 2020 onwards highlighted.

Language: Английский

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Guidelines and definitions for research on epithelial–mesenchymal transition

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(6), P. 341 - 352

Published: April 16, 2020

Epithelial-mesenchymal transition (EMT) encompasses dynamic changes in cellular organization from epithelial to mesenchymal phenotypes, which leads functional cell migration and invasion. EMT occurs a diverse range of physiological pathological conditions is driven by conserved set inducing signals, transcriptional regulators downstream effectors. With over 5,700 publications indexed Web Science 2019 alone, research on expanding rapidly. This growing interest warrants the need for consensus among researchers when referring undertaking EMT. Consensus Statement, mediated 'the International Association' (TEMTIA), outcome 2-year-long discussion aims both clarify nomenclature provide definitions guidelines future publications. We trust that these will help reduce misunderstanding misinterpretation data generated various experimental models promote cross-disciplinary collaboration identify address key open questions this field. While recognizing importance maintaining diversity approaches conceptual frameworks, we emphasize lasting contributions increasing our understanding developmental processes combatting cancer other diseases depend adoption unified terminology describe

Language: Английский

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Tumor-Associated Macrophages in Tumor Immunity

Frontiers in Immunology, Journal Year: 2020, Volume and Issue: 11

Published: Dec. 3, 2020

Tumor-associated macrophages (TAMs) represent one of the main tumor-infiltrating immune cell types and are generally categorized into either two functionally contrasting subtypes, namely classical activated M1 alternatively M2 macrophages. The former typically exerts anti-tumor functions, including directly mediate cytotoxicity antibody-dependent cell-mediated (ADCC) to kill tumor cells; latter can promote occurrence metastasis cells, inhibit T response, angiogenesis, lead progression. Both have high degree plasticity thus be converted each other upon microenvironment changes or therapeutic interventions. As relationship between TAMs malignant tumors becoming clearer, become a promising target for developing new cancer treatment. In this review, we summarize origin TAMs, interaction with microenvironment, up-to-date treatment strategies targeting TAMs.

Language: Английский

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EMT, MET, Plasticity, and Tumor Metastasis

Trends in Cell Biology, Journal Year: 2020, Volume and Issue: 30(10), P. 764 - 776

Published: Aug. 13, 2020

Language: Английский

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Mechanisms of 3D cell migration

Nature Reviews Molecular Cell Biology, Journal Year: 2019, Volume and Issue: 20(12), P. 738 - 752

Published: Oct. 3, 2019

Language: Английский

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TGFβ biology in cancer progression and immunotherapy

Nature Reviews Clinical Oncology, Journal Year: 2020, Volume and Issue: 18(1), P. 9 - 34

Published: July 24, 2020

Language: Английский

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Circulating tumor cells: biology and clinical significance

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: Nov. 22, 2021

Abstract Circulating tumor cells (CTCs) are that have sloughed off the primary and extravasate into circulate in blood. Understanding of metastatic cascade CTCs has tremendous potential for identification targets against cancer metastasis. Detecting these very rare among massive blood is challenging. However, emerging technologies detection profoundly contributed to deepening investigation biology facilitated their clinical application. Current summarized herein, together with advantages disadvantages. The usually dependent on molecular markers, epithelial cell adhesion molecule being most widely used, although markers vary between different types cancer. Properties associated epithelial-to-mesenchymal transition stemness been identified CTCs, indicating increased capacity. Only a small proportion can survive eventually initiate metastases, suggesting an interaction modulation hostile microenvironment essential CTC Single-cell sequencing extensively investigated, enabled researchers reveal genome transcriptome CTCs. Herein, we also review applications especially monitoring response treatment evaluating prognosis. Hence, will continue contribute providing significant insights processes open new avenues useful applications.

Language: Английский

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Interleukins in cancer: from biology to therapy

Nature reviews. Cancer, Journal Year: 2021, Volume and Issue: 21(8), P. 481 - 499

Published: June 3, 2021

Language: Английский

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Dynamic EMT: a multi‐tool for tumor progression

The EMBO Journal, Journal Year: 2021, Volume and Issue: 40(18)

Published: Aug. 30, 2021

Review30 August 2021Open Access Dynamic EMT: a multi-tool for tumor progression Simone Brabletz Corresponding Author [email protected] orcid.org/0000-0003-0936-1526 Department of Experimental Medicine 1, Nikolaus-Fiebiger Center Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany Search more papers by this author Harald Schuhwerk orcid.org/0000-0001-6971-3760 Thomas orcid.org/0000-0003-2983-9048 Marc P. Stemmler orcid.org/0000-0002-7866-3686 Information *,1, Schuhwerk1, Brabletz1 and *,1 1Department *Corresponding author. Tel: +49 9131 85 29101; E-mail: The EMBO Journal (2021)40:e108647https://doi.org/10.15252/embj.2021108647 This article is part the Cancer Reviews 2021 series. PDFDownload PDF text main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract process epithelial–mesenchymal transition (EMT) fundamental embryonic morphogenesis. Cells undergoing it lose epithelial characteristics integrity, acquire mesenchymal features, become motile. In cancer, program hijacked confer essential changes in morphology motility that fuel invasion. addition, EMT increasingly understood orchestrate large variety complementary cancer such as cell stemness, tumorigenicity, resistance therapy adaptation microenvironment. review, we summarize recent findings related these various classical non-classical functions, introduce true tumorigenic multi-tool, involved many aspects cancer. We suggest therapeutic targeting will—if acknowledging complexities—be possibility concurrently interfere with on levels. Introduction Epithelial-to-mesenchymal describes transdifferentiation stationary cells mesenchymal, motile phenotype was initially observed early development (Hay, 1995). Here, contributes embryonal processes like gastrulation, neural crest formation, or heart (Thiery et al, 2009; Nieto 2016). also crucial physiological wound healing (Arnoux 2008) tissue homeostasis (Ahmed 2006). Importantly, pathological reactivation plays role diseases organ fibrosis metastasis (Fig 1A), which focus review. Figure 1. Classical functions (A) frequently occurs at invasive front tumors, destroys well-defined structures, allows migrate, invade tissue, intravasate blood lymphatic vessels. Tumor their way through body can travel single cells, clusters exhibiting partial headed leader cell. At secondary site, extravasate colonize distant organ, where MET outgrowth macrometastases. (B) induced mainly set transcription factors (EMT-TFs) ZEB1, ZEB2, SNAIL, SLUG TWIST differ protein structure, size, individual functions. All them are repressors E-cadherin activate markers Vimentin, Fibronectin N-cadherin. Epithelial displaying apical–basal polarity held together tight junctions, adherens desmosomes anchored underlying basement membrane hemidesmosomes. They express three different complexes junctional molecules maintain polarity. EMT, expression EMT-TFs leads inhibition major components structures concomitantly activates genes associated state. gain front–rear polarity, display actin stress fibers, capacities. Notably, very rarely switch completely phenotype, but fluently convert between intermediate states certain features keeping sets characteristics. Further, reversible process. Mesenchymal revert state MET. An important execution played microRNAs miR-200 mir-34 families regulated double-negative feedback loops ZEB1/2 respectively, serve reinforce either Download figure PowerPoint an extremely complex diverse disease not only varying entities, within same entity, subtypes, even subtypes. tumors spatial temporal heterogeneity be elicited, e.g., via occurrence consecutive mutations clonal evolution (McGranahan Swanton, 2017). However, plasticity, allowing continuous adaption ever-changing conditions, mediated genetically fixed, depending accumulating mutations, epigenetically orchestrated signals from microenvironment, rendering whole (by activating mesenchymal–epithelial transition; MET) highly dynamic (see overview Fig 2). 2. Overview summarizing multiple oncogenic course progression. allow invade, intra- sites, enables traits support initiation well metastatic colonization. Throughout progression, they help cope changing conditions metabolic reprogramming, enhanced survival altered DNA repair prevention death, immune evasion improved chemo- radiotherapy. supporting handle environmental extracellular from, CAFs microenvironment approaches. executed core EMT-activating including SNAIL (also SNAI1) SNAI2), basic helix–loop–helix TWIST1 (TWIST) TWIST2 zinc finger E-Box binding homeobox ZEB1 ZEB2. share ability repress encoding gene CDH1 motifs cognate promoter regions (Nieto 2016) shown (Batlle 2000; Cano 2000), (Yang 2004), (Eger 2005), ZEB2 (Comijn 2001). parallel, directly indirectly VIM (Vimentin), FN1 (Fibronectin), CDH2 (N-cadherin) 2016; Dongre Weinberg, 2019) 1B). shared, distinct EMT-TFs, patterns size structure (Stemmler 2019). Beyond "classical" traits, motility, capacities, widespread importance biology indicated additional pleiotropic (Brabletz 2018). have been stemness properties increase linking concept stem (CSCs). Additionally, repair, escape senescence apoptosis, resistance, resulting pro-survival providing advantage under types conditions. Altogether, non-redundant context-dependent dynamically (TME) permanently adapt (Puisieux 2014). Consequently, intervention EMT/plasticity will provide opportunity fight blow. all highlight clinical implications. Classical/core Migration invasion normal form protective sheets structural integrity. connection junction junction, desmosomes, junctions seal located apical constitute barrier solutes water. apical-basal function, has defined "asymmetry" tissues. Polarity complexes, Par, Crumbs, Scribble ensure proper organization versus basolateral domains (Huang 2012). Of note, some control regulate spindle orientation division mode (Martin-Belmonte Perez-Moreno, 2011) Elicited TME, activation toward malignancy, accompanied substantial cellular Cell–cell contacts deconstructed repression CDH1, cadherin (E-cadherin), constituent coding other molecules. As consequence disintegration direct transcriptional several members Crumbs lost (Aigner 2007; Moreno-Bueno 2008; Spaderna Lamouille 2014) coincides profound cytoskeletal reorganization constriction, formation conversion cuboidal columnar shapes spindle-like elongated forms (Moreno-Bueno 2008). Newly formed actin-rich protrusions lamellipodia filopodia movement. To surrounding tissues, induce invadopodia, specialized proteolytic function (Yilmaz Christofori, Eckert 2011; Ridley, Sundararajan 2015). supported induction matrix metalloproteases degradation adjacent tissues (Miyoshi 2004; Miyoshi 2005; Huang 2009) inducers prevent synthesis repressing its (Spaderna events cause loss integrity dissemination thus execute first step cascade 1A). MET: colonization 1990, Fearon Vogelstein proposed meanwhile genetic model colorectal tumorigenesis. described deterioration greater malignancy driven stepwise accumulation hypothesized perpetuates during last established malignant carcinoma metastases, implying metastases most degenerated (Fearon Vogelstein, 1990). efforts identify specific metastasis-associated remained unsuccessful. Rather, already twenty years ago, compared de-differentiated nature primary tumor, exhibit re-differentiated morphology, similar center These led hypothesis de-differentiation transient condition opposing re-differentiation needs initiated advantageous macrometastases (Figs 1A But why do re-differentiate? Invasive, were growth arrested, whereas proliferation detected metastasis, suggesting must reversed order fact inhibit 3). There publications confirming relevance (Chaffer 2006; Korpal Ocana 2012; Tsai perfect accordance failure EMT-causing attributed epigenetic regulation 3. Cellular plasticity governed provided window phenotypes cells. Drug sensitivity, proliferation, response apoptosis highest states, drug efflux, invasion, states. A hybrid provides maximal capacity, changes. Note extreme initiation, lost. Whereas investigated great detail below), less known about trigger reverse Is just lack EMT-inducing stimuli coupled reduced EMT-TFs? Several studies could show knockdown EMT-TF sufficient elicit vitro lines entities depletion Zeb1 mouse pancreatic fixes (Krebs Once ZEB-family (ZEB1 ZEB2) declines, reduction reinforced loop family microRNAs. During ZEB transcriptionally members. Vice versa, post-transcriptional level Thus, bidirectional transitions potentiated ZEB/miR-200 circuit (Bracken Burk Wellner 2009). branch inducible tumor-suppressor p53, miR-200s (Kim 2011). Upregulation consequences. It exerts both invasion- migration-inhibiting, tumor-suppressing (Peter, 2009), promotes (Korpal controversial, EMT-MET adapting respective Similar negative loop, miR-34 regulatory (Siemens Diaz-Lopez target themselves, BMI1, CD44, CD133, JAG1, MYC tumor-relevant "non-classical" discussed below Brabletz, 2010; Since slow multistep take requires different, sometimes apparently spatiotemporal manner, valid investigation remains challenging rely models. Recently, focusing EMT/MET using elegant 2012, al (2012) demonstrated induction, case TWIST, supports skin subsequent Twist1 downregulation necessary (Tsai 2012) Another study necessity MMTV-PyMT breast disseminate lung. EMT-state niche local fibroblasts turn (MET) (del Pozo Martin Esposito colleagues found E-selectin adhesion bone vascular elicits (Esposito summary, reports significance detailed still need further investigation. Partial long viewed binary separate populations. past, narrow perspective challenged means metastasis. One example analysis Fischer Fsp1-Cre lineage tracing Based finding lung consist had never switched full Fsp1+ authors concluded (Fischer nowadays accepted that, although reactivated types, fully end-stage Vimentin often expressed. rather gradual incomplete, termed Pastushenko Blanpain, 2019; Yang 2020) Over years, report vivo detection carrying combination markers. Already 1990s, analyses reported observations (Mareel 1992; Birchmeier Behrens, 1994). Later, circulating (CTCs) simultaneous (Yu 2013). Similarly, identified co-expression (EpCAM+) (Vim+) marker autochthonous murine prostate (Ruscetti linked single-cell transcriptomics head neck (Puram group occurring introduced term "hybrid" (Pastushenko 2018; 2021) Moreover, Bornes used (2015) incapable detecting majority disseminating partial/hybrid 2015; does mean important. evidence traps profoundly suppresses mammary (Ye oncogene-induced model, Xu (2017) required small subset (Xu Interestingly, modes involve levels observed. groups forming clusters. Indeed, type "collective migration" might common than dissemination, approaches clustered circulation (Friedl Aceto 2014; Cheung Nevertheless, despite appearance, characteristic detectable migrating (Aiello 2018), follow "leader" pave "follower" (Matise Chen Non-classical Besides drive phenotypes, regulating tumorigenesis Regulation Normal dependent source replenish dying committed terminally differentiated tissue. observation maintained after transplantations into mice, prompted (Reya Simplified, measured capability fractions mice. Strikingly, capacity increasing Shibue 2017; Wilson Overexpression SNAI1, TWIST1, CD44+/CD24lo pool, increased sphere vitro, elevated tumorigenicity (Mani Morel determines healthy gland converts luminal settings (Guo results obtained key determinant reciprocal ZEB1/miR-200 controlling BMI1 SOX2 (Shimono Krebs squamous (SCC) cooperatively CDKN2A (p16INK4A) promote capacities 2010). protocadherin FAT1 one player stemness. inactivated SCC involving CAMK2, SRC activities nuclear translocation YAP1 2021). another promoting factor, PRRX1, thereby uncoupling EMT/migration (Ocana PRRX1 isoform switching driving force (Takano Mechanistically, realm correlate gradually efficiencies seeding line idea addition (Shibue 2013; 2020), transformation. upregulation RAS transformed bronchial unleash favoring aggressive undifferentiated (Morel Liu 2014b; Larsen KRAS dependency (KRAS addiction) thresholds KRAS-dependent (Singh 2014b). effects evident ectopic Zeb2 intestinal epithelium transgenic Elevation generates absence cooperating defects (Slowicka 2020). Therapy Loss durable efficacy relapse initial successful treatment obstacles battle against Conventional favorably eliminating non-stem cell-like fails deplete properties. Settleman, Santamaria Dudas signatures acquisition strongly correlated, standard targeted EGFR PI3K inhibitors (Creighton Farmer Byers For example, gemcitabine-resistant Panc1 sensitized upon (Wellner routes include efflux evading anoikis former ATP-binding cassette (ABC) transporter family, FOXC1 (Aller Singh Saxena contribute therapy-induced interfering p53 PTEN, BCL-XL (Vega Escriva Kurrey Wu Cao Experimentally HMLER 10-fold IC50 doses chemotherapeutics (Gupta Tulchinsky experiments, GFP-labeled PyMT high cyclophosphamide non-small-cell (NSCLC), AXL receptor tyrosine kinase inhibition, sustained activity Sequist Zhang Furthermore, HDAC class I demethylation resensitizes osteosarcoma chemotherapy (Meidhof

Language: Английский

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