Cell stem cell,
Journal Year:
2024,
Volume and Issue:
31(3), P. 359 - 377.e10
Published: March 1, 2024
Mitochondrial
fatty
acid
oxidation
(FAO)
is
essential
for
hematopoietic
stem
cell
(HSC)
self-renewal;
however,
the
mechanism
by
which
mitochondrial
metabolism
controls
HSC
fate
remains
unknown.
Here,
we
show
that
within
lineage,
HSCs
have
largest
NADPH
pools,
are
required
proper
and
homeostasis.
Bioinformatic
analysis
of
transcriptome,
biochemical
assays,
genetic
inactivation
FAO
all
indicate
FAO-generated
fuels
cholesterol
synthesis
in
HSCs.
Interference
with
disturbs
segregation
toward
corresponding
daughter
cells
upon
single
division.
Importantly,
found
FAO-NADPH-cholesterol
axis
drives
extracellular
vesicle
(EV)
biogenesis
release
HSCs,
while
inhibition
EV
signaling
impairs
self-renewal.
These
data
reveal
existence
a
NADPH-cholesterol
homeostasis
highlight
non-stochastic
nature
determination.
European Journal of Immunology,
Journal Year:
2019,
Volume and Issue:
49(10), P. 1457 - 1973
Published: Oct. 1, 2019
These
guidelines
are
a
consensus
work
of
considerable
number
members
the
immunology
and
flow
cytometry
community.
They
provide
theory
key
practical
aspects
enabling
immunologists
to
avoid
common
errors
that
often
undermine
immunological
data.
Notably,
there
comprehensive
sections
all
major
immune
cell
types
with
helpful
Tables
detailing
phenotypes
in
murine
human
cells.
The
latest
techniques
applications
also
described,
featuring
examples
data
can
be
generated
and,
importantly,
how
analysed.
Furthermore,
tips,
tricks
pitfalls
avoid,
written
peer-reviewed
by
leading
experts
field,
making
this
an
essential
research
companion.
Signal Transduction and Targeted Therapy,
Journal Year:
2022,
Volume and Issue:
7(1)
Published: Aug. 6, 2022
Abstract
Recent
advancements
in
stem
cell
technology
open
a
new
door
for
patients
suffering
from
diseases
and
disorders
that
have
yet
to
be
treated.
Stem
cell-based
therapy,
including
human
pluripotent
cells
(hPSCs)
multipotent
mesenchymal
(MSCs),
has
recently
emerged
as
key
player
regenerative
medicine.
hPSCs
are
defined
self-renewable
types
conferring
the
ability
differentiate
into
various
cellular
phenotypes
of
body,
three
germ
layers.
MSCs
progenitor
possessing
self-renewal
(limited
vitro)
differentiation
potential
lineages,
according
International
Society
Cell
Gene
Therapy
(ISCT).
This
review
provides
an
update
on
recent
clinical
applications
using
either
or
derived
bone
marrow
(BM),
adipose
tissue
(AT),
umbilical
cord
(UC)
treatment
diseases,
neurological
disorders,
pulmonary
dysfunctions,
metabolic/endocrine-related
reproductive
skin
burns,
cardiovascular
conditions.
Moreover,
we
discuss
our
own
trial
experiences
targeted
therapies
setting,
propose
MSC
origin
concept
how
may
contribute
role
downstream
applications,
with
ultimate
objective
facilitating
translational
research
medicine
applications.
The
mechanisms
discussed
here
support
proposed
hypothesis
BM-MSCs
potentially
good
candidates
brain
spinal
injury
treatment,
AT-MSCs
disorder
regeneration,
UC-MSCs
disease
acute
respiratory
distress
syndrome
treatment.
European Journal of Immunology,
Journal Year:
2021,
Volume and Issue:
51(12), P. 2708 - 3145
Published: Dec. 1, 2021
Abstract
The
third
edition
of
Flow
Cytometry
Guidelines
provides
the
key
aspects
to
consider
when
performing
flow
cytometry
experiments
and
includes
comprehensive
sections
describing
phenotypes
functional
assays
all
major
human
murine
immune
cell
subsets.
Notably,
contain
helpful
tables
highlighting
differences
between
cells.
Another
useful
feature
this
is
analysis
clinical
samples
with
examples
applications
in
context
autoimmune
diseases,
cancers
as
well
acute
chronic
infectious
diseases.
Furthermore,
there
are
detailing
tips,
tricks
pitfalls
avoid.
All
written
peer‐reviewed
by
leading
experts
immunologists,
making
an
essential
state‐of‐the‐art
handbook
for
basic
researchers.
Cancers,
Journal Year:
2021,
Volume and Issue:
13(2), P. 217 - 217
Published: Jan. 9, 2021
Multiple
myeloma
(MM)
is
a
hematologic
cancer
characterized
by
clonal
proliferation
of
plasma
cells
in
the
bone
marrow
(BM).
The
progression,
from
early
stages
disease
as
monoclonal
gammopathy
undetermined
significance
(MGUS)
and
smoldering
multiple
(SMM)
to
MM
occasionally
extramedullary
disease,
drastically
affected
tumor
microenvironment
(TME).
Soluble
factors
direct
cell–cell
interactions
regulate
cell
trafficking
homing
BM
niche.
Mesenchymal
stromal
cells,
osteoclasts,
osteoblasts,
myeloid
lymphoid
present
create
unique
milieu
that
favors
immune
evasion
promotes
progression.
Moreover,
TME
implicated
malignant
protection
against
anti-tumor
therapy.
This
review
describes
main
cellular
non-cellular
components
located
BM,
which
condition
immunosuppressive
environment
lead
establishment
