Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

Nature Reviews Microbiology, Journal Year: 2019, Volume and Issue: 18(2), P. 67 - 83

Published: Dec. 19, 2019

Language: Английский

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(7), P. 824 - 844

Published: June 22, 2020

Language: Английский

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Human organoids: model systems for human biology and medicine

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(10), P. 571 - 584

Published: July 7, 2020

Language: Английский

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The promise and challenge of therapeutic genome editing

Nature, Journal Year: 2020, Volume and Issue: 578(7794), P. 229 - 236

Published: Feb. 12, 2020

Language: Английский

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RNA-based therapeutics: an overview and prospectus

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(7)

Published: July 23, 2022

Abstract The growing understanding of RNA functions and their crucial roles in diseases promotes the application various RNAs to selectively function on hitherto “undruggable” proteins, transcripts genes, thus potentially broadening therapeutic targets. Several RNA-based medications have been approved for clinical use, while others are still under investigation or preclinical trials. Various techniques explored promote intracellular trafficking metabolic stability, despite significant challenges developing therapeutics. In this review, mechanisms action, challenges, solutions, therapeutics comprehensively summarized.

Language: Английский

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Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations

Genes, Journal Year: 2020, Volume and Issue: 11(8), P. 854 - 854

Published: July 26, 2020

Being the most common musculoskeletal progressive condition, osteoarthritis is an interesting target for research. It estimated that prevalence of knee (OA) among adults 60 years age or older approximately 10% in men and 13% women, making OA one leading causes disability elderly population. Today, we know not a disease characterized by loss cartilage due to mechanical loading only, but condition affects all tissues joint, causing detectable changes tissue architecture, its metabolism function. All these are mediated complex yet fully researched interplay proinflammatory anti-inflammatory cytokines, chemokines, growth factors adipokines, which can be measured serum, synovium histological samples, potentially serving as biomarkers stage progression. Another key aspect progression epigenome regulates genetic expression through DNA methylation, histone modifications, mRNA interference. A lot work has been put into developing non-surgical treatment options slow down natural course postpone, maybe even replace extensive surgeries such total arthroplasty. At moment, biological treatments platelet-rich plasma, bone marrow mesenchymal stem cells autologous microfragmented adipose containing stromal vascular fraction ordinarily used. Furthermore, latter two mentioned cell-based seem only methods so far increase quality patients. Yet, future, gene therapy could become option orthopedic In following review, summarized latest important research basic sciences, pathogenesis, non-operative treatment.

Language: Английский

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CRISPR technologies for precise epigenome editing

Nature Cell Biology, Journal Year: 2021, Volume and Issue: 23(1), P. 11 - 22

Published: Jan. 1, 2021

Language: Английский

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Engineering microrobots for targeted cancer therapies from a medical perspective

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Nov. 5, 2020

Abstract Systemic chemotherapy remains the backbone of many cancer treatments. Due to its untargeted nature and severe side effects it can cause, numerous nanomedicine approaches have been developed overcome these issues. However, targeted delivery therapeutics challenging. Engineering microrobots is increasingly receiving attention in this regard. Their functionalities, particularly their motility, allow penetrate tissues reach cancers more efficiently. Here, we highlight how different microrobots, ranging from tailor-made motile bacteria tiny bubble-propelled microengines hybrid spermbots, be engineered integrate sophisticated features optimised for precision-targeting a wide range cancers. Towards this, importance integrating clinicians, public patients early on development novel technologies.

Language: Английский

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Prime editing for precise and highly versatile genome manipulation

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 24(3), P. 161 - 177

Published: Nov. 7, 2022

Language: Английский

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CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

Nano Letters, Journal Year: 2020, Volume and Issue: 21(1), P. 693 - 699

Published: Dec. 21, 2020

Cell-free DNA (cfDNA) has attracted significant attention due to its high potential diagnose diseases, such as cancer. Still, detection by amplification method limitations because of false-positive signals and difficulty in designing target-specific primers. CRISPR-Cas-based fluorescent biosensors have been developed but also need the step for detection. In this study, first time CRISPR-Cas12a based nucleic acid amplification-free biosensor was detect cfDNA a metal-enhanced fluorescence (MEF) using DNA-functionalized Au nanoparticle (AuNP). Upon activating complex target subsequent single-strand (ssDNA) degradation between AuNP fluorophore, MEF occurred with color changes from purple red-purple. Using system, breast cancer gene-1 (BRCA-1) can be detected very sensitivity 30 min. This rapid highly selective sensor applied measure other biomarkers viral field-deployable point-of-care testing (POCT) platform.

Language: Английский

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