Nanomechanical Signatures of Extracellular Vesicles from Hematologic Cancer Patients Unraveled by Atomic Force Microscopy for Liquid Biopsy DOI

Yaqi Feng,

Meichen Liu, Xinxin Li

et al.

Nano Letters, Journal Year: 2023, Volume and Issue: 23(4), P. 1591 - 1599

Published: Feb. 1, 2023

Cells release extracellular vesicles (EVs) as the carriers for intercellular communications to regulate life activities. Particularly, it is increasingly apparent that mechanical forces play an essential role in biological systems. The nanomechanical properties of EVs and their dynamics cancer development are still not fully understood. Herein, with use atomic force microscopy (AFM), signatures from liquid biopsies hematologic patients were unraveled. Single native probed by AFM under aqueous conditions. elastic viscous measured visualized correlate EV mechanics geometry. Experimental results remarkably reveal significant differences among multiple myeloma patients, lymphoma healthy volunteers. study unveils unique cancers, which will benefit studies diagnosis prognosis translational significance.

Language: Английский

Extracellular vesicle analysis DOI
An Hendrix, Lien Lippens, Cláudio Pinheiro

et al.

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: July 27, 2023

Language: Английский

Citations

85
Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications DOI Creative Commons
Liwei Wang, Di Wang, Zhaoming Ye

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

Citations

66
Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade DOI Creative Commons
Saeid Moghassemi, Arezoo Dadashzadeh, Maria João Sousa

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 126 - 156

Published: March 2, 2024

Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.

Language: Английский

Citations

33
A Panorama of Extracellular Vesicle Applications: From Biomarker Detection to Therapeutics DOI Creative Commons
S. Jerome Das, Christopher J. Lyon, Tony Hu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(14), P. 9784 - 9797

Published: March 12, 2024

Extracellular vesicles (EVs) secreted by all cell types are involved in the cell-to-cell transfer of regulatory factors that influence and tissue phenotypes normal diseased tissues. EVs thus a rich source biomarker targets for assays analyze blood urinary disease diagnosis. Sensitive detection derived from specific populations is key major hurdle when analyzing complex biological samples, but innovative approaches surveyed this Perspective can streamline EV isolation enhance sensitivity procedures required clinical application EV-based diagnostics therapeutics, including nanotechnology microfluidics, to achieve characterizations. Finally, also outlines opportunities challenges remaining translation assays.

Language: Английский

Citations

32
Extracellular Vesicles and Immunity: At the Crossroads of Cell Communication DOI Open Access
Noemi Aloi, Gaspare Drago, Silvia Ruggieri

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1205 - 1205

Published: Jan. 18, 2024

Extracellular vesicles (EVs), comprising exosomes and microvesicles, are small membranous structures secreted by nearly all cell types. They have emerged as crucial mediators in intercellular communication, playing pivotal roles diverse physiological pathological processes, notably within the realm of immunity. These go beyond mere cellular interactions, extracellular stand versatile dynamic components immune regulation, impacting both innate adaptive Their multifaceted involvement includes activation, antigen presentation, immunomodulation, emphasising their significance maintaining homeostasis contributing to pathogenesis immune-related disorders. participate immunomodulation delivering a wide array bioactive molecules, including proteins, lipids, nucleic acids, thereby influencing gene expression target cells. This manuscript presents comprehensive review that encompasses vitro vivo studies aimed at elucidating mechanisms through which EVs modulate human Understanding intricate interplay between immunity is imperative for unveiling novel therapeutic targets diagnostic tools applicable various immunological disorders, autoimmune diseases, infectious cancer. Furthermore, recognising potential drug delivery vehicles holds significant promise future immunotherapies.

Language: Английский

Citations

28
Cell Type-Specific Extracellular Vesicles and Their Impact on Health and Disease DOI Open Access
Sohil Amin,

Hamed Massoumi,

Deepshikha Tewari

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2730 - 2730

Published: Feb. 27, 2024

Extracellular vesicles (EVs), a diverse group of cell-derived exocytosed particles, are pivotal in mediating intercellular communication due to their ability selectively transfer biomolecules specific cell types. EVs, composed proteins, nucleic acids, and lipids, taken up by cells affect variety signaling cascades. Research the field has primarily focused on stem with particular focus mesenchymal cells, for potential therapeutic benefits. Recently, tissue-specific EVs or type-specific extracellular (CTS-EVs), have garnered attention unique biogenesis molecular composition because they enable highly targeted cell-specific communication. Various studies outlined roles that CTS-EVs play physiological function maintenance homeostasis, including immune modulation, tissue regeneration, organ development. These properties also exploited disease propagation, such as cancer, neurological disorders, infectious diseases, autoimmune conditions, more. The insights gained from analyzing different biological not only enhance our understanding pathogenesis but open new avenues innovative diagnostic biomarkers targets wide spectrum medical conditions. This review comprehensively outlines current CTS-EV origins, within normal physiology, implications diseased states.

Language: Английский

Citations

20
Lipophilicity Modulation of Fluorescent Probes for In Situ Imaging of Cellular Microvesicle Dynamics DOI
Wei Huang, Guangmei Han, Dong Wang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Real-time monitoring of dynamic microvesicles (MVs), vesicles associated with living cells, is great significance in deeply understanding their origin, transport, and function. However, specific labeling MVs poses a challenge due to the lack unique biomarkers that differentiate them from other cellular compartments. Here, we present strategy selectively label by evaluating series lipid layer-sensitive cationic indolium-coumarin fluorescent probes (designated as IC-Cn, n ranging 1 18) feature varying aliphatic side chains (CnH2n+1). Through situ cell imaging analysis, found IC-Cn location highly related lipophilicities phospholipid layer hydrophobic microenvironments In detail, IC-C1 IC-C2 specifically localize both inside outside cells. contrast, IC-C3, IC-C4, IC-C5 mitochondria but distinct fluorescence lifetimes. Using these strategically, have discovered that, addition biogenesis plasma membranes damaged mitochondria, newly formed can undergo fusion fission processes. Moreover, mitochondria-derived MVs, beyond being released parent fuse lysosomes facilitate removal dysfunctional mitochondria. The work not only provides new insights into MV physiology also inspires design strategies for used studies.

Language: Английский

Citations

2
Cell and gene therapy for kidney disease DOI
Jennifer L. Peek, Matthew H. Wilson

Nature Reviews Nephrology, Journal Year: 2023, Volume and Issue: 19(7), P. 451 - 462

Published: March 27, 2023

Language: Английский

Citations

31
A 3D culture system improves the yield of MSCs-derived extracellular vesicles and enhances their therapeutic efficacy for heart repair DOI Open Access
Ling Sun, Yuan Ji, Boyu Chi

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 161, P. 114557 - 114557

Published: March 22, 2023

Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs), due to their inner functional substances, have shown great value in treating acute myocardial infarction (AMI). However, clinical application is limited by a low yield. In the present study, we cultured EVs using hollow fiber bioreactor-based three-dimensional (3D) system, and assessed therapeutic effectiveness on AMI.The MSCs separated fresh human umbilical cord were planted into flasks of two systems: two-dimensional (2D) culture hollow-fiber-bioreactor based 3D culture. extracted supernatants. Characteristics yields systems, namely 2D-EVs 3D-EVs, compared. A rat model AMI was built up assess efficacy yield 3D-EVs higher, with biofunctions similar those 2D-EVs. repressed apoptosis cardiomyocytes, facilitated angiogenesis, regulated transition macrophage subpopulations after infarction, eventually improved cardiac function rats.The system can increase MSCs-derived render strong cardioprotective effect rats.

Language: Английский

Citations

27
Emerging principles of primary cilia dynamics in controlling tissue organization and function DOI Creative Commons
Jay Gopalakrishnan, Kerstin Feistel, Benjamin M. Friedrich

et al.

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(21)

Published: Sept. 25, 2023

Abstract Primary cilia project from the surface of most vertebrate cells and are key in sensing extracellular signals locally transducing this information into a cellular response. Recent findings show that primary not merely static organelles with distinct lipid protein composition. Instead, function relies on dynamic composition molecules within cilium, context‐dependent processing stimuli, cycles assembly disassembly cell‐ tissue‐specific manner. Thereby, dynamically integrate different inputs control cell fate during tissue development. Here, we review recently emerging concept dynamics development, organization, remodeling, function.

Language: Английский

Citations

25