Association between arterial stiffness, carbamylation, and mortality in patients undergoing coronary angiography with no or mild chronic kidney disease DOI Creative Commons

C. Daschner,

Marcus E. Kleber, Ksenija Stach

et al.

Cardiorenal Medicine, Journal Year: 2024, Volume and Issue: 15(1), P. 83 - 97

Published: Dec. 19, 2024

Introduction: The processes of atherosclerosis, inflammation, and carbamylation are closely linked in cardiovascular (CV) disease, but the potential burden as a CV mortality predictor is unclear, especially patients with no or mild chronic kidney disease (CKD). This study aimed to investigate whether elevated carbamylated albumin (C-Alb), surrogate marker for burden, associated arterial stiffness/atherosclerotic CKD, using pulse pressure (PP) stiffness. Methods: We measured C-Alb 3,193 participants Ludwigshafen Risk Cardiovascular Health who had been referred coronary angiography followed up 10 years. Results: mean age was 62.7 years, 30.4% were female. Mean blood 141/81 mm Hg, 5.54 mmol/mol. Increase levels older age; female sex; increased PP, high-sensitivity C-reactive protein, interleukin-6 levels; incidence artery (CAD), peripheral (PAD), carotid stenosis. In contrast, BMI, diastolic (DBP), albumin, proportion active smokers decreased increasing levels. particular, showed highly significant correlation CAD severity: Friesinger (Pearson coefficient [r] = 0.082, p < 0.001) Gensini score (r 0.066, 0.001). area under curve (AUC) all-cause prediction by European Society Cardiology Heart Score (ESC-HS) significantly improved from 0.719 0.735, AUC based on 0.726 0.750 without previously known disease. correlated directly PP 0.062, 0.001), which consistently strongest across all tertiles. hazard ratios (HRs) per Hg increase (or 1,000 Hg/min double product [DP]) 1st tertile 1.18, 1.13, 1.11, 1.11 (MAP), systolic (SBP), DP, respectively, HR DBP did not reach significance. 3rd C-Alb, HRs 1.05, 1.09, SBP, MAP Conclusion: may be valuable biomarker assessing risk improving even CKD. findings support notion crosslink between carbamylation, mortality. While these results promising, further research needed fully elucidate role progression stratification.

Language: Английский

Advanced oxidation protein products induce apoptosis in thyroid follicular epithelial cells through oxidative stress in Hashimoto’s thyroiditis DOI
Jie Tan,

Ruoting Ding,

Shitong Yu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 148, P. 114069 - 114069

Published: Jan. 18, 2025

Language: Английский

Citations

1
Extracellular microvesicles from patients with Rheumatoid arthritis promote dendritic cell activation in vitro DOI Creative Commons
Brigitta Buttari,

Serena Recalchi,

Gloria Riitano

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Rheumatoid Arthritis (RA) is a systemic autoimmune disease characterized by chronic synovial inflammation affecting diarthrodial joints, with cartilage destruction and bone erosion. Environmental inflammatory stimuli can induce maturation of dendritic cells (DCs), which promote differentiation activation effector T lymphocytes. We previously highlighted the role extracellular microvesicles (EMVs) in pathogenesis carrying antigens that trigger autoantibody production. In this investigation we verified whether EMVs may activate immature monocyte-derived DCs, inducing phenotypic functional characteristics mature DCs. were obtained from 7 RA patients naïve to biological disease-modifying anti-rheumatic drugs (DMARDs) tested for their capability DCs healthy donors. preliminary confirmed western blot carbamylated citrullinated proteins are present patients. Moreover, surface marker phenotyping indicated EMV treated-DCs exhibit increased expression CD83 CD86, as well CD83+ HLA-DR+ CD80+ CD86+ cells, indicating state. Furthermore, biochemical data demonstrated plasma MAPK NF-κB also able stimulate produce IL-12, IL-1β IL-10, proinflammatory phenotype. These findings demonstrate DC vitro, suggesting potential mechanism microenvironment perpetuates through modulation function. knowledges provide new insight therapeutic targets.

Language: Английский

Citations

0
Vitamin K preserves gamma‐glutamyl carboxylase activity against carbamylations in uremia: Implications for vascular calcification and adjunct therapies DOI Creative Commons
Nadine Kaesler, Sadasivam Kaushik, Janina Frisch

et al.

Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(5)

Published: April 9, 2025

Abstract Aim Vascular calcification contributes to morbidity and mortality in aging is accelerated diabetes chronic kidney disease. Matrix Gla Protein a potent inhibitor of vascular calcification, which activated by the vitamin K‐dependent gamma‐glutamyl carboxylase (GGCX). However, through currently unidentified mechanism, activity GGCX reduced experimental uremia, thereby contributing promotion calcifications. In this study, we aim identify cause these functional alterations stimulate enzyme potential binding compounds as new avenue therapy. Methods Two rodent models uremia human carotid plaques were assessed for modifications, well calcification. silico compound screening via BindScope identified partners further validated assays enzymatic changes vitro Mass spectrometry was applied monitor molecular mass GGCX. Results analysis revealed post‐translational modifications uremic rats mice, calcified plaques. Functional showed that carbamylation activity, prevented K2. Chrysin, screening, stimulated calcium deposition VSMCs, oxidized at lysine 517. Conclusion conclusion, study clearly demonstrates plays significant role may be modulated help prevent pathological changes.

Language: Английский

Citations

0
Crosstalk between glomeruli and tubules DOI
Agnes B. Fogo, Raymond C. Harris

Nature Reviews Nephrology, Journal Year: 2024, Volume and Issue: 21(3), P. 189 - 199

Published: Dec. 6, 2024

Language: Английский

Citations

4
Guanidinylation compromises the anti-inflammatory and anti-oxidative properties of apolipoprotein A-I in chronic kidney disease progression DOI Creative Commons

Andrea Bonnin-Marquez,

Joachim Jankowski,

Sanne L. Maas

et al.

Kidney International, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
The combined predictive power of the atherogenic index of plasma and serum glycated albumin for cardiovascular events in postmenopausal patients with acute coronary syndrome after percutaneous coronary intervention DOI Creative Commons
Xunxun Feng,

Yang Liu,

Jiaqi Yang

et al.

Lipids in Health and Disease, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 30, 2024

Glycated Albumin (GA) and atherogenic index of plasma (AIP) are two important biomarkers that respectively reflect lipid glucose levels. Previous research has revealed their roles in cardiovascular diseases (CVD) diabetes. However, combined predictive ability forecasting events (CVE) after percutaneous coronary intervention (PCI) among postmenopausal acute syndrome (ACS) patients remains insufficiently studied.

Language: Английский

Citations

2
Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives DOI
Limei Zhang, Hao Bai, Jie Zou

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, ultraweak biosignals presents significant challenges. This has spurred a rapid development analytical techniques aiming for increased sensitivity specificity. These advancements, including signal amplification strategies integration biorecognition events, mark transformative era in bioanalytical precision accuracy. A prominent method among these innovations is immuno‐rolling circle (immuno‐RCA) technology, which effectively combines immunoassays with via RCA. process starts when targeted biomolecule, such as protein or cell, binds to an immobilized antibody probe on substrate. The introduction circular DNA template triggers RCA, leading exponential significantly enhanced intensity, thus target molecule detectable quantifiable even at single‐molecule level. review provides overview biosensing strategy extensive practical applications immuno‐RCA biomarkers. Furthermore, it scrutinizes limitations inherent sensors sets forth expectations their future trajectory. serves valuable reference advancing various domains, diagnostics, biomarker discovery, molecular imaging.

Language: Английский

Citations

1
Carbamylation—A Pathologic Posttranslational Modification Affecting Platelet and Von Willebrand Factor Function during Uremic Kidney Disease DOI Creative Commons
Rory R. Koenen

Thrombosis and Haemostasis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Language: Английский

Citations

1
Diphtheria Toxoid-Derived T-Helper Epitope and α-galactosylceramide Synergistically Enhance the Immunogenicity of Glycopeptide Antigen DOI Creative Commons
Jingjing Du, Shihao Zhou,

Jin Liu

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(12), P. 3889 - 3901

Published: Nov. 4, 2024

The tumor-associated antigen MUC1 is an attractive target for immunotherapy, however, its weak immunogenicity limits the induction of antitumor immune responses. To overcome this limitation, in study, glycopeptide was covalently linked with a diphtheria toxin-derived T-helper epitope (DT331–345). Subsequently, resulting DT-MUC1 physically mixed natural killer T cell agonist αGalCer to explore their immunomodulatory synergy. Biological results demonstrated that compared MUC1+αGalCer and groups, specific IgG antibody titer DT-MUC1+αGalCer group increased by 189- 3-fold, respectively, indicating synergistically enhanced αGalCer. Moreover, vaccine induced potent cellular responses significantly inhibited growth B16-MUC1 tumors vivo. Furthermore, it found anti-MUC1 equivalent palmitoylated (P1-DT-MUC1+αGalCer) higher than doubly (P2-DT-MUC1+αGalCer), suggesting easily synthesized may not require lipid chain modification already possess good amphiphilicity. This first time helper enhance immunogenicity, study provide effective design strategy MUC1-targeted vaccines offer novel insights into fully synthetic peptide vaccines.

Language: Английский

Citations

1
Crosstalk between periodontitis and cardiovascular risk DOI Creative Commons
Ulrike Schulze‐Späte,

Ludwig Wurschi,

Emiel P. C. van der Vorst

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 9, 2024

Recent demographic developments resulted in an aged society with a rising disease burden of systemic and non-communicable diseases (NCDs). In cardiovascular (CVD), NCD high morbidity mortality, recent preventive strategies include the investigation comorbidities to reduce its significant economic burden. Periodontal disease, oral bacterial-induced inflammatory tooth-supporting tissue, is regulated prevalence severity by individual host response dysbiotic microbiota. Clinically, both NCDs are highly associated; however, shared risk factors such as smoking, obesity, type II diabetes mellitus chronic stress represent only insufficient explanation for multifaceted interactions entities. Specifically, crosstalk between not yet fully understood. This review summarizes current knowledge on clinical association periodontitis CVD, elaborates how periodontitis-induced pathophysiological mechanisms patients may contribute increased focus atherosclerosis. Clinical implications well future therapy considerations discussed. Overall, this supports novel scientific endeavors aiming at improving quality life comprehensive integrated approach improve well-being aging populations worldwide.

Language: Английский

Citations

1