Journal of Cellular and Molecular Medicine,
Journal Year:
2023,
Volume and Issue:
27(12), P. 1653 - 1663
Published: May 2, 2023
Abstract
High‐mobility
group
box1
(HMGB1)
induces
inflammatory
injury,
and
emerging
reports
suggest
that
it
is
critical
for
brain
ischemia
reperfusion.
Engeletin,
a
natural
Smilax
glabra
rhizomilax
derivative,
reported
to
possess
anti‐inflammatory
activity.
Herein,
we
examined
the
mechanism
of
engeletin‐mediated
neuroprotection
in
rats
having
transient
middle
cerebral
artery
occlusion
(tMCAO)
against
reperfusion
injury.
Male
SD
were
induced
using
1.5
h
tMCAO,
following
by
22.5
h.
Engeletin
(15,
30
or
60
mg/kg)
was
intravenously
administered
immediately
0.5
ischemia.
Based
on
our
results,
engeletin,
dose‐dependent
fashion,
reduced
neurological
deficits,
infarct
size,
histopathological
alterations,
edema
factors,
namely,
circulating
IL‐1β,
TNF‐α,
IL‐6
IFN‐γ.
Furthermore,
engeletin
treatment
markedly
neuronal
apoptosis,
which,
turn,
elevated
Bcl‐2
protein
levels,
while
suppressing
Bax
Cleaved
Caspase‐3
levels.
Meanwhile,
significantly
reduces
overall
expressions
HMGB1,
TLR4,
NF‐κB
attenuated
nuclear
transfer
factor
kappa
B
(NF‐κB)
p65
ischemic
cortical
tissue.
In
conclusion,
strongly
prevents
focal
via
suppression
HMGB1/TLR4/NF‐κB
network.
MedComm,
Journal Year:
2023,
Volume and Issue:
4(4)
Published: July 31, 2023
Multi-omics
usually
refers
to
the
crossover
application
of
multiple
high-throughput
screening
technologies
represented
by
genomics,
transcriptomics,
single-cell
proteomics
and
metabolomics,
spatial
so
on,
which
play
a
great
role
in
promoting
study
human
diseases.
Most
current
reviews
focus
on
describing
development
multi-omics
technologies,
data
integration,
particular
disease;
however,
few
them
provide
comprehensive
systematic
introduction
multi-omics.
This
review
outlines
existing
technical
categories
multi-omics,
cautions
for
experimental
design,
focuses
integrated
analysis
methods
especially
approach
machine
learning
deep
integration
corresponding
tools,
medical
researches
(e.g.,
cancer,
neurodegenerative
diseases,
aging,
drug
target
discovery)
as
well
open-source
tools
databases,
finally,
discusses
challenges
future
directions
precision
medicine.
With
algorithms,
important
disease
research,
also
provided
detailed
introduction.
will
guidance
researchers,
who
are
just
entering
into
research.
MedComm,
Journal Year:
2023,
Volume and Issue:
4(3)
Published: May 2, 2023
Protein
posttranslational
modifications
(PTMs)
refer
to
the
breaking
or
generation
of
covalent
bonds
on
backbones
amino
acid
side
chains
proteins
and
expand
diversity
proteins,
which
provides
basis
for
emergence
organismal
complexity.
To
date,
more
than
650
types
protein
modifications,
such
as
most
well-known
phosphorylation,
ubiquitination,
glycosylation,
methylation,
SUMOylation,
short-chain
long-chain
acylation
redox
irreversible
have
been
described,
inventory
is
still
increasing.
By
changing
conformation,
localization,
activity,
stability,
charges,
interactions
with
other
biomolecules,
PTMs
ultimately
alter
phenotypes
biological
processes
cells.
The
homeostasis
important
human
health.
Abnormal
may
cause
changes
in
properties
loss
functions,
are
closely
related
occurrence
development
various
diseases.
In
this
review,
we
systematically
introduce
characteristics,
regulatory
mechanisms,
functions
health
addition,
therapeutic
prospects
diseases
by
targeting
associated
enzymes
also
summarized.
This
work
will
deepen
understanding
promote
discovery
diagnostic
prognostic
markers
drug
targets
Theranostics,
Journal Year:
2022,
Volume and Issue:
12(15), P. 6740 - 6761
Published: Jan. 1, 2022
Extracellular
vesicles
(EVs),
of
which
exosomes
are
a
representative
subgroup,
naturally
secreted
nanoparticles
with
variety
payloads.With
the
intrinsic
merits
stability,
biocompatibility,
low
immunogenicity,
and
large
capacity,
EVs
widely
regarded
as
effective
carriers
drug
delivery.However,
disadvantages,
such
yield,
complicated
isolation
procedures,
loading
efficiency,
hinder
its
clinical
translation.In
this
review,
we
systematically
summarize
advances
in
EV
(especially
exosomes)
engineering
for
application,
focusing
on
strategies
toward
high
facile
isolation,
efficient
cargo
loading,
improved
delivery,
optimized
manufacturing,
might
unleash
infinite
power
translation.
Circulation Research,
Journal Year:
2022,
Volume and Issue:
130(8), P. 1145 - 1166
Published: April 14, 2022
Stroke
remains
a
leading
cause
of
death
and
disability,
with
limited
therapeutic
options
suboptimal
tools
for
diagnosis
prognosis.
High
throughput
technologies
such
as
proteomics
generate
large
volumes
experimental
data
at
once,
thus
providing
an
advanced
opportunity
to
improve
the
status
quo
by
facilitating
identification
novel
targets
molecular
biomarkers.
Proteomics
studies
in
animals
are
largely
designed
decipher
pathways
altered
brain
tissue
after
stroke,
whereas
human
patients
primarily
focus
on
biomarker
discovery
biofluids
and,
more
recently,
thrombi
extracellular
vesicles.
Here,
we
offer
comprehensive
review
stroke
conducted
both
animal
specimen
present
our
view
limitations,
challenges,
future
perspectives
field.
In
addition,
unique
resource
scientific
community,
provide
extensive
lists
all
proteins
identified
proteomic
perform
postanalysis
reveal
stroke-related
cellular
processes
pathways.
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
13(8), P. 3238 - 3251
Published: May 23, 2023
Emerging
evidence
has
demonstrated
the
vital
role
of
metabolism
in
various
diseases
or
disorders.
Metabolomics
provides
a
comprehensive
understanding
biological
systems.
With
advanced
analytical
techniques,
metabolomics
exhibits
unprecedented
significant
value
basic
drug
research,
including
disease
mechanisms,
identifying
targets,
and
elucidating
mode
action
drugs.
More
importantly,
greatly
accelerates
development
process
by
predicting
pharmacokinetics,
pharmacodynamics,
response.
In
addition,
facilitates
exploration
repurposing
drug-drug
interactions,
as
well
personalized
treatment
strategies.
Here,
we
briefly
review
recent
advances
technologies
update
our
knowledge
applications
research
development.
