Microscale Neuronal Activity Collectively Drives Chaotic and Inflexible Dynamics at the Macroscale in Seizures DOI Creative Commons

Dominic R. W. Burrows,

Giovanni Diana,

Birgit Pimpel

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(18), P. 3259 - 3283

Published: April 5, 2023

Neuronal activity propagates through the network during seizures, engaging brain dynamics at multiple scales. Such propagating events can be described avalanches framework, which relate spatiotemporal microscale with global properties. Interestingly, in healthy networks are indicative of critical dynamics, where is organized to a phase transition, optimizes certain computational Some have hypothesized that pathologic epileptic seizures an emergent property neuronal collectively driving away from criticality. Demonstrating this would provide unifying mechanism linking dysfunction seizures. Here, we investigated effect drug-induced on avalanche using

Language: Английский

Anakinra Promotes M2 Microglia Activation during the Latent Phase of the Lithium-Pilocarpine Model of Temporal Lobe Epilepsy DOI
Maria V. Zakharova, A. V. Dyomina, Anna A. Kovalenko

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 60(2), P. 672 - 689

Published: March 1, 2024

Language: Английский

Citations

5
The astrocyte α1A‐adrenoreceptor is a key component of the neuromodulatory system in mouse visual cortex DOI Creative Commons
Jérôme Wahis, Cansu Akkaya, Andre M. Kirunda

et al.

Glia, Journal Year: 2024, Volume and Issue: 72(11), P. 1955 - 1973

Published: July 12, 2024

Abstract Noradrenaline (norepinephrine) is known to modulate many physiological functions and behaviors. In this study, we tested what extent astrocytes, a type of glial cell, participate in noradrenergic signaling mouse primary visual cortex (V1). Astrocytes are essential partners neurons the central nervous system. They brain homeostasis, but also dynamically regulate neuronal activity, notably by relaying regulating neuromodulator signaling. Indeed, astrocytes express receptors for multiple neuromodulators, including noradrenaline, which involved remains unclear. To test whether neuromodulation mice, employed both short hairpin RNA mediated knockdown as well pharmacological manipulation major noradrenaline receptor α1A‐adrenoreceptor. Using acute slices, found that astrocytic α1A‐adrenoreceptor subtype contributes generation large intracellular Ca 2+ signals generally thought underlie astrocyte function. if reduced affected function circuits V1, used patch‐clamp field potential recordings. These revealed through important not only synaptic activity plasticity potentiation responses information processing.

Language: Английский

Citations

5
Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis DOI

Zixian Zhou,

Pengcheng Zhang,

Dongshan Ya

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 132, P. 155892 - 155892

Published: July 17, 2024

Language: Английский

Citations

5
Single-cell, single-nucleus and xenium-based spatial transcriptomics analyses reveal inflammatory activation and altered cell interactions in the hippocampus in mice with temporal lobe epilepsy DOI Creative Commons

Quanlei Liu,

Chunhao Shen,

Yang Dai

et al.

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Sept. 13, 2024

Abstract Background Temporal lobe epilepsy (TLE) is among the most common types of and often leads to cognitive, emotional, psychiatric issues due frequent seizures. A notable pathological change related TLE hippocampal sclerosis (HS), which characterized by neuronal loss, gliosis, an increased neuron fibre density. The mechanisms underlying TLE-HS development remain unclear, but reactive transcriptomic changes in glial cells neurons hippocampus post-epileptogenesis may provide insights. Methods To induce TLE, 200 nl kainic acid (KA) was stereotactically injected into CA1 region mice, followed a 7-day postinjection period. Single-cell RNA sequencing (ScRNA-seq), single-nucleus (SnRNA-seq), Xenium-based spatial transcriptomics analyses were employed evaluate mRNA expression neurons. Results From ScRNA-seq SnRNA-seq data, 31,390 48,221 nuclei identified. Analysis differentially expressed genes (DEGs) revealed significant alterations mice with affecting hundreds thousands mRNAs their signalling pathways. Enrichment analysis indicated activation stress inflammatory pathways hippocampus, while axonal neural support suppressed. Xenium demonstrated all 247 across mouse brain sections, revealing distributions 27 cell types. Integrated DEGs identified via three techniques that Spp1 , Trem2 Cd68 upregulated data; Penk Sorcs3 Plekha2 Tle4 Sipa1l3 downregulated data. Conclusion In this study, high-resolution single-cell atlas established, potential intrinsic driving TLE-associated altered interactions. These findings valuable insights for further exploration HS epileptogenesis.

Language: Английский

Citations

5
Microscale Neuronal Activity Collectively Drives Chaotic and Inflexible Dynamics at the Macroscale in Seizures DOI Creative Commons

Dominic R. W. Burrows,

Giovanni Diana,

Birgit Pimpel

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(18), P. 3259 - 3283

Published: April 5, 2023

Neuronal activity propagates through the network during seizures, engaging brain dynamics at multiple scales. Such propagating events can be described avalanches framework, which relate spatiotemporal microscale with global properties. Interestingly, in healthy networks are indicative of critical dynamics, where is organized to a phase transition, optimizes certain computational Some have hypothesized that pathologic epileptic seizures an emergent property neuronal collectively driving away from criticality. Demonstrating this would provide unifying mechanism linking dysfunction seizures. Here, we investigated effect drug-induced on avalanche using

Language: Английский

Citations

13