Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
28, P. 386 - 401
Published: June 16, 2023
Organoids
are
in
vitro
model
systems
that
mimic
the
complexity
of
organs
with
multicellular
structures
and
functions,
which
provide
great
potential
for
biomedical
tissue
engineering.
However,
their
current
formation
heavily
relies
on
using
complex
animal-derived
extracellular
matrices
(ECM),
such
as
Matrigel.
These
often
poorly
defined
chemical
components
exhibit
limited
tunability
reproducibility.
Recently,
biochemical
biophysical
properties
hydrogels
can
be
precisely
tuned,
offering
broader
opportunities
to
support
development
maturation
organoids.
In
this
review,
fundamental
ECM
vivo
critical
strategies
design
organoid
culture
summarized.
Two
typically
derived
from
natural
synthetic
polymers
applicability
improve
organoids
presented.
The
representative
applications
incorporating
into
highlighted.
Finally,
some
challenges
future
perspectives
also
discussed
developing
advanced
technologies
toward
supporting
research.
Advanced Functional Materials,
Journal Year:
2023,
Volume and Issue:
33(19)
Published: Feb. 22, 2023
Abstract
Multicellular
spheroids
and
organoids
are
promising
in
vitro
3D
models
personalized
medicine
drug
screening.
They
replicate
the
structural
functional
characteristics
of
human
organs
vivo.
Microfluidic
technology
micro‐nano
fabrication
can
fulfill
high
requirement
engineering
approach
growing
research
interest
organoids.
In
this
review,
comparatively
introduced.
Then
it
is
illustrated
how
spheroids‐
organoids‐on‐a‐chip
facilitates
their
establishment,
expansion,
application
through
spatial‐temporal
control,
mechanical
cues
modeling,
high‐throughput
analysis,
co‐culture,
multi‐tissue
interactions,
biosensing,
bioimaging
integration.
The
potential
opportunities
challenges
developing
finally
outlooked.
Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(5), P. 112322 - 112322
Published: April 26, 2023
Crosstalk
between
cardiac
cells
is
critical
for
heart
performance.
Here
we
show
that
vascular
within
human
organoids
(hCOs)
enhance
their
maturation,
force
of
contraction,
and
utility
in
disease
modeling.
Herein
optimize
our
protocol
to
generate
populations
addition
epicardial,
fibroblast,
cardiomyocyte
self-organize
into
in-vivo-like
structures
hCOs.
We
identify
mechanisms
communication
endothelial
cells,
pericytes,
fibroblasts,
cardiomyocytes
ultimately
contribute
organoid
maturation.
In
particular,
(1)
endothelial-derived
LAMA5
regulates
expression
mature
sarcomeric
proteins
contractility,
(2)
paracrine
platelet-derived
growth
factor
receptor
β
(PDGFRβ)
signaling
from
upregulates
matrix
deposition
augment
hCO
contractile
force.
Finally,
demonstrate
determine
the
magnitude
diastolic
dysfunction
caused
by
inflammatory
factors
a
role
endothelin
driving
dysfunction.
Together
this
study
highlights
importance
models.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: May 30, 2023
Abstract
Three-dimensional
hydrogel-based
organ-like
cultures
can
be
applied
to
study
development,
regeneration,
and
disease
in
vitro.
However,
the
control
of
engineered
hydrogel
composition,
mechanical
properties
geometrical
constraints
tends
restricted
initial
time
fabrication.
Modulation
characteristics
over
according
culture
evolution
is
often
not
possible.
Here,
we
overcome
these
limitations
by
developing
a
hydrogel-in-hydrogel
live
bioprinting
approach
that
enables
dynamic
fabrication
instructive
elements
within
pre-existing
cultures.
This
achieved
crosslinking
photosensitive
hydrogels
via
two-photon
absorption
at
any
during
culture.
We
show
guide
neural
axon
directionality
growing
organotypic
spinal
cords,
geometry
differential
cell
migration
cancer
organoids.
Finally,
promote
polarity
liver
organoids,
small
intestinal
organoid
morphogenesis
lung
tip
bifurcation
composition
shape.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(4), P. 4089 - 4089
Published: Feb. 17, 2023
The
intestine
is
considered
to
be
a
vital
digestive
organ
absorb
nutrients
and
the
largest
immune
organ,
while
numerous
microorganisms
coexist
with
host.
It
well
known
that
complex
interactions
between
gut
microbiota
host’s
system
inevitably
affect
function
of
other
organs,
creating
an
“axis”
them.
During
past
few
years,
new
technique
based
mainly
on
microfluidics
cell
biology
has
been
developed
emulate
structure,
function,
microenvironment
human
gut,
called
“gut-on-chip”.
This
microfluidic
chip
provides
insight
into
key
aspects
in
health
disease,
such
as
gut–brain
axis,
gut–liver
gut–kidney
gut–lung
axis.
In
this
review,
we
first
describe
basic
theory
axis
various
composition
parameter
monitoring
microarray
systems,
summarize
development
emerging
advances
gut–organ-on-chip,
focus
host-gut
flora
nutrient
metabolism,
highlight
their
role
pathophysiological
studies.
addition,
paper
discusses
challenges
prospects
for
current
further
use
gut–organ-on-chip
platform.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
28, P. 386 - 401
Published: June 16, 2023
Organoids
are
in
vitro
model
systems
that
mimic
the
complexity
of
organs
with
multicellular
structures
and
functions,
which
provide
great
potential
for
biomedical
tissue
engineering.
However,
their
current
formation
heavily
relies
on
using
complex
animal-derived
extracellular
matrices
(ECM),
such
as
Matrigel.
These
often
poorly
defined
chemical
components
exhibit
limited
tunability
reproducibility.
Recently,
biochemical
biophysical
properties
hydrogels
can
be
precisely
tuned,
offering
broader
opportunities
to
support
development
maturation
organoids.
In
this
review,
fundamental
ECM
vivo
critical
strategies
design
organoid
culture
summarized.
Two
typically
derived
from
natural
synthetic
polymers
applicability
improve
organoids
presented.
The
representative
applications
incorporating
into
highlighted.
Finally,
some
challenges
future
perspectives
also
discussed
developing
advanced
technologies
toward
supporting
research.
