Functional connectomics spanning multiple areas of mouse visual cortex

Nature, Journal Year: 2025, Volume and Issue: 640(8058), P. 435 - 447

Published: April 9, 2025

Abstract Understanding the brain requires understanding neurons’ functional responses to circuit architecture shaping them. Here we introduce MICrONS connectomics dataset with dense calcium imaging of around 75,000 neurons in primary visual cortex (VISp) and higher areas (VISrl, VISal VISlm) an awake mouse that is viewing natural synthetic stimuli. These data are co-registered electron microscopy reconstruction containing more than 200,000 cells 0.5 billion synapses. Proofreading a subset yielded reconstructions include complete dendritic trees as well local inter-areal axonal projections map up thousands cell-to-cell connections per neuron. Released open-access resource, this includes tools for retrieval analysis 1,2 . Accompanying studies describe its use comprehensive characterization cell types 3–6 , synaptic level connectivity diagram cortical column 4 uncovering cell-type-specific inhibitory can be linked gene expression 4,7 Functionally, identify new computational principles how information integrated across space 8 characterize novel neuronal invariances 9 bring structure function together uncover general principle between excitatory within 10,11

Language: Английский

---

A collicular map for touch-guided tongue control

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

---

The behavioral mechanisms governing collective motion in swarming locusts

Science, Journal Year: 2025, Volume and Issue: 387(6737), P. 995 - 1000

Published: Feb. 27, 2025

Collective motion, which is ubiquitous in nature, has traditionally been explained by “self-propelled particle” models from theoretical physics. Here we show, through field, lab, and virtual reality experimentation, that classical of collective behavior cannot account for how motion emerges marching desert locusts, whose swarms affect the livelihood millions. In contrast to assumptions made these models, locusts do not explicitly align with neighbors. While individuals respond moving-dot stimuli optomotor response, this innate does mediate social response Instead, locust behavior, across scales, can be a minimal cognitive framework, incorporates individuals’ neural representation bearings neighbors internal consensus dynamics making directional choices. Our findings challenge long-held beliefs about order emerge disorder animal collectives.

Language: Английский

---

Theory of the Multiregional Neocortex: Large-Scale Neural Dynamics and Distributed Cognition

Annual Review of Neuroscience, Journal Year: 2022, Volume and Issue: 45(1), P. 533 - 560

Published: July 8, 2022

The neocortex is a complex neurobiological system with many interacting regions. How these regions work together to subserve flexible behavior and cognition has become increasingly amenable rigorous research. Here, I review recent experimental theoretical on the modus operandi of multiregional cortex. These studies revealed several general principles for neocortical interareal connectivity, low-dimensional macroscopic gradients biological properties across cortical areas, hierarchy timescales information processing. Theoretical suggests testable predictions regarding differential excitation inhibition along feedforward feedback pathways in hierarchy. Furthermore, modeling distributed working memory simple decision-making given rise novel mathematical concept, dubbed bifurcation space, that potentially explains how different canonical circuit organization but heterogeneities, are able their respective (e.g., sensory coding versus executive control) functions modularly organized brain.

Language: Английский

---

Flexible navigational computations in the Drosophila central complex

Current Opinion in Neurobiology, Journal Year: 2022, Volume and Issue: 73, P. 102514 - 102514

Published: Feb. 19, 2022

Language: Английский

---

Walking strides direct rapid and flexible recruitment of visual circuits for course control in Drosophila

Neuron, Journal Year: 2022, Volume and Issue: 110(13), P. 2124 - 2138.e8

Published: May 6, 2022

Flexible mapping between activity in sensory systems and movement parameters is a hallmark of motor control. This flexibility depends on the continuous comparison short-term postural dynamics longer-term goals an animal, thereby necessitating neural mechanisms that can operate across multiple timescales. To understand how such body-brain interactions emerge timescales to control movement, we performed whole-cell patch recordings from visual neurons involved course Drosophila. We show leg mechanosensory cells, propagating via specific ascending neurons, critical for stride-by-stride steering adjustments driven by circuit, and, at longer timescales, it provides information about moving body's state flexibly recruit circuit Thus, our findings demonstrate presence elegant stride-based mechanism operating context-dependent propose this functions as general basis adaptive locomotion.

Language: Английский

---

Connectomics and the neural basis of behaviour

Current Opinion in Insect Science, Journal Year: 2022, Volume and Issue: 54, P. 100968 - 100968

Published: Sept. 13, 2022

Methods to acquire and process synaptic-resolution electron-microscopy datasets have progressed very rapidly, allowing production annotation of larger, more complete connectomes. More accurate neuronal matching techniques are enriching cell type data with gene expression, neuron activity, behaviour developmental information, providing ways test hypotheses circuit function. In a variety behaviours such as learned innate olfaction, navigation sexual behaviour, connectomics has already revealed interconnected modules hierarchical structure, recurrence integration sensory streams. Comparing individual connectomes determine which features robust variable is one key research area; new work in comparative across development, experience, sex species will establish strong links between connectivity brain

Language: Английский

---

Visual processing in the fly, from photoreceptors to behavior

Genetics, Journal Year: 2023, Volume and Issue: 224(2)

Published: May 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Language: Английский

---

A rise-to-threshold process for a relative-value decision

Nature, Journal Year: 2023, Volume and Issue: 619(7970), P. 563 - 571

Published: July 5, 2023

Abstract Whereas progress has been made in the identification of neural signals related to rapid, cued decisions 1–3 , less is known about how brains guide and terminate more ethologically relevant which an animal’s own behaviour governs options experienced over minutes 4–6 . Drosophila search for many seconds egg-laying sites with high relative value 7,8 have neurons, called oviDNs, whose activity fulfills necessity sufficiency criteria initiating egg-deposition motor programme 9 Here we show that oviDNs express a calcium signal (1) dips when egg internally prepared (ovulated), (2) drifts up down minutes—in manner influenced by substrates—as fly determines whether lay (3) reaches consistent peak level just before abdomen bend deposition. This apparent cell bodies brain it probably reflects behaviourally rise-to-threshold process ventral nerve cord, where synaptic terminals are located their output can influence behaviour. We provide perturbational evidence initiated once this hits threshold subthreshold variation regulates time spent considering and, ultimately, choice taken. Finally, identify small recurrent circuit feeds into each its constituent types required laying egg. These results argue relative-value, self-paced decision initial insight underlying mechanism building process.

Language: Английский

---

Global change in brain state during spontaneous and forced walk in Drosophila is composed of combined activity patterns of different neuron classes

eLife, Journal Year: 2023, Volume and Issue: 12

Published: April 17, 2023

Movement-correlated brain activity has been found across species and regions. Here, we used fast whole lightfield imaging in adult Drosophila to investigate the relationship between walk brain-wide neuronal activity. We observed a global change that tightly correlated with spontaneous bouts of walk. While specific sets excitatory, inhibitory, neuromodulatory neurons highlighted their joint contribution, spatial heterogeneity walk- turning-induced allowed parsing unique responses from subregions sometimes individual candidate neurons. For example, previously uncharacterized serotonergic were inhibited during onset some areas preceded exclusively spontaneously walking animals, forced elicited similar most These data suggest major contribution walk-related sensory or proprioceptive information all classes.

Language: Английский

---