A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Oct. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Language: Английский

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Building an allocentric travelling direction signal via vector computation

Nature, Journal Year: 2021, Volume and Issue: 601(7891), P. 92 - 97

Published: Dec. 15, 2021

Language: Английский

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Neuronal wiring diagram of an adult brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Language: Английский

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Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster

Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2574 - 2594.e23

Published: May 1, 2024

High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.

Language: Английский

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A neural circuit for wind-guided olfactory navigation

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 8, 2022

Abstract To navigate towards a food source, animals frequently combine odor cues about source identity with wind direction location. Where and how these two are integrated to support navigation is unclear. Here we describe pathway the Drosophila fan-shaped body that encodes attractive promotes upwind navigation. We show neurons throughout this encode odor, but not direction. Using connectomics, identify local called h∆C receive input from previously described pathway. exhibit odor-gated, direction-tuned activity, sparse activation of in reproducible direction, activity required for persistent orientation during odor. Based on connectome data, develop computational model showing can promote goal such as an source. Our results suggest processed by separate pathways within goal-directed

Language: Английский

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Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 27, 2023

Abstract The fruit fly Drosophila melanogaster combines surprisingly sophisticated behaviour with a highly tractable nervous system. A large part of the fly’s success as model organism in modern neuroscience stems from concentration collaboratively generated molecular genetic and digital resources. As presented our FlyWire companion paper 1 , this now includes first full brain connectome an adult animal. Here we report systematic hierarchical annotation ∼130,000-neuron including neuronal classes, cell types developmental units (hemilineages). This enables any researcher to navigate huge dataset find systems neurons interest, linked literature through Virtual Fly Brain database 2 . Crucially, resource 4,552 types. 3,094 are rigorous consensus validations previously proposed “hemibrain” 3 In addition, propose 1,458 new types, arising mostly fact that spans whole brain, whereas hemibrain derives subvolume. Comparison showed type counts strong connections were largely stable, but connection weights variable within across animals. Further analysis defined simple heuristics for interpretation: stronger than 10 unitary synapses or providing >1% input target conserved. Some increased variability connectomes: most common mushroom body, required learning memory, is almost twice numerous hemibrain. We evidence functional homeostasis adjustments absolute amount excitatory while maintaining excitation-inhibition ratio. Finally, surprisingly, about one third could not yet be reliably identified connectome. therefore suggest should robust inter-individual variation, namely groups cells quantitatively more similar different other same brain. Joint connectomes demonstrates viability utility definition. Our work defines atlas provides both intellectual framework open source toolchain brain-scale comparative connectomics.

Language: Английский

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Converting an allocentric goal into an egocentric steering signal

Nature, Journal Year: 2024, Volume and Issue: 626(8000), P. 808 - 818

Published: Feb. 7, 2024

Neuronal signals that are relevant for spatial navigation have been described in many species

Language: Английский

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Transforming a head direction signal into a goal-oriented steering command

Nature, Journal Year: 2024, Volume and Issue: 626(8000), P. 819 - 826

Published: Feb. 7, 2024

To navigate, we must continuously estimate the direction are headed in, and correct deviations from our goal

Language: Английский

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Mapping the neural dynamics of locomotion across the Drosophila brain

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 710 - 726.e4

Published: Jan. 21, 2024

Language: Английский

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A Drosophila computational brain model reveals sensorimotor processing

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

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