Cell–extracellular matrix mechanotransduction in 3D

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 495 - 516

Published: Feb. 27, 2023

Language: Английский

---

Extracellular fluid viscosity enhances cell migration and cancer dissemination

Nature, Journal Year: 2022, Volume and Issue: 611(7935), P. 365 - 373

Published: Nov. 2, 2022

Abstract Cells respond to physical stimuli, such as stiffness 1 , fluid shear stress 2 and hydraulic pressure 3,4 . Extracellular viscosity is a key cue that varies under physiological pathological conditions, cancer 5 However, its influence on biology the mechanism by which cells sense changes in are unknown. Here we demonstrate elevated counterintuitively increases motility of various cell types two-dimensional surfaces confinement, dissemination from three-dimensional tumour spheroids. Increased mechanical loading imposed induces an actin-related protein 2/3 (ARP2/3)-complex-dependent dense actin network, enhances Na + /H exchanger (NHE1) polarization through actin-binding partner ezrin. NHE1 promotes swelling increased membrane tension, which, turn, activates transient receptor potential cation vanilloid 4 (TRPV4) mediates calcium influx, leading RHOA-dependent contractility. The coordinated action remodelling/dynamics, NHE1-mediated RHOA-based contractility facilitates enhanced at viscosities. Breast pre-exposed acquire TRPV4-dependent memory transcriptional control Hippo pathway, migration zebrafish, extravasation chick embryos lung colonization mice. Cumulatively, extracellular regulates both short- long-term cellular processes with pathophysiological relevance biology.

Language: Английский

---

Engineered hydrogels for mechanobiology

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: Dec. 15, 2022

Language: Английский

---

Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Jan. 19, 2023

Abstract Across complex, multi-time and -length scale biological systems, redundancy confers robustness resilience, enabling adaptation increasing survival under dynamic environmental conditions; this review addresses ubiquitous effects of cytoskeletal remodelling, triggered by biomechanical, biophysical biochemical cues, on stem cell mechanoadaptation emergent lineage commitment. The cytoskeleton provides an adaptive structural scaffold to the cell, regulating emergence structure-function relationships during tissue neogenesis, both in prenatal development as well postnatal healing. Identification mapping mechanical cues conducive remodelling may help establish contexts that can be used prospectively translational design specifications target neogenesis for regenerative medicine. In review, we summarize findings context early healing, its relevance guiding commitment targeted regeneration. We highlight how cytoskeleton-targeting chemical agents modulate differentiation govern responses cells’ emerging form function. further methods spatiotemporal visualization measurement properties cells, a function adaptation. Research these areas facilitate translation own healing potential improve materials, therapies, devices

Language: Английский

---

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(52)

Published: April 23, 2023

Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural biological complexity within tissues that is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from molecular whole organ level. Current techniques materials employed imposed limits on scale, speed, resolution can be achieved, rendering technique reproduce hierarchies cell-matrix interactions are observed bone. The shift toward biomimetic approaches bone engineering, where hydrogels provide biophysical biochemical cues encapsulated cells, promising approach enhancing function development vitro modeling. A major focus modeling creating dynamic microenvironmental niches support, stimulate, direct cellular processes formation remodeling. Hydrogels ideal imitating extracellular matrix since they engineered present various whilst allowing bioprinting. Here, recent advances 3D niche conducive engineering models reviewed.

Language: Английский

---

Mechanical state transitions in the regulation of tissue form and function

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(8), P. 654 - 670

Published: April 10, 2024

Language: Английский

---

Matrix mechano-sensing at the invasive front induces a cytoskeletal and transcriptional memory supporting metastasis

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 14, 2025

The extracellular matrix (ECM) controls tumour dissemination. We characterise ECM organization in human and mouse tumours, identifying three regions: body, proximal invasive front distal front. Invasive areas show increased density, fibre thickness, length, alignment, with unique radial orientation at the correlating amoeboid features. Using patient samples murine models, we find that metastases recapitulate features of primary tumour. Ex vivo culture cancer cells isolated from different regions reveals a spatial cytoskeletal transcriptional memory. Several vitro models organisation showing induces 3D confinement supporting Rho-ROCK-Myosin II activity, while enhances directional invasion. Spatial transcriptomics identifies mechano-inflammatory program associated worse prognosis across multiple types. These findings provide mechanistic insights into how shapes local invasion distant metastasis. Interactions control tumor proliferation, Here, authors information on it influences cell metastasis properties through induction

Language: Английский

---

Self-generated gradients steer collective migration on viscoelastic collagen networks

Nature Materials, Journal Year: 2022, Volume and Issue: 21(10), P. 1200 - 1210

Published: May 30, 2022

Language: Английский

---

Programming hydrogels to probe spatiotemporal cell biology

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(5), P. 678 - 691

Published: April 11, 2022

Language: Английский

---

Mechanics of the cellular microenvironment as probed by cells in vivo during zebrafish presomitic mesoderm differentiation

Nature Materials, Journal Year: 2022, Volume and Issue: 22(1), P. 135 - 143

Published: Dec. 28, 2022

Tissue morphogenesis, homoeostasis and repair require cells to constantly monitor their three-dimensional microenvironment adapt behaviours in response local biochemical mechanical cues. Yet the parameters of cellular probed by vivo remain unclear. Here, we report mechanics that probe situ during zebrafish presomitic mesoderm differentiation. By quantifying both endogenous cell-generated strains tissue mechanics, show individual stiffness associated with deformations supracellular, foam-like architecture. Stress relaxation leads a perceived decreases over time, probing softest regime. We find most parameters, including those cells, vary along anteroposterior axis as mesodermal progenitors differentiate. These findings expand our understanding mechanosensation might aid design advanced scaffolds for engineering applications.

Language: Английский

---