The
treatment
of
severe
bone
defects
remains
an
unresolved
clinical
challenge.
Injectable
hydrogels
loaded
with
drugs
or
growth
factors
are
considered
to
offer
substantial
benefits
in
the
regeneration
irregular
shapes
under
complex
pathological
microenvironments.
In
this
study,
all-silk-derived
composite
hydrogel
was
developed
for
regeneration.
methacrylated
silk
fibroin
(SilMA)
fabricated
situ
injection
and
photocuring
serve
as
a
supporting
matrix,
incorporation
platelet-rich
plasma
(PRP)
promote
migration
pre-differentiation
marrow
mesenchymal
stem
cells
(BMSCs)
during
early
stage,
(SF)
microspheres
encapsulating
berberine
(BBR)
regulate
BMSCs
osteogenesis
over
extended
period.
were
demonstrated
by
upregulating
mitochondrial
biogenesis
fusion.
Additionally,
it
indicated
that
impaired
osteogenic
activity
dynamics
inflammatory
conditions
can
be
reversed
through
addition
SF-BBR
microspheres.
Rat
calvarial
defect
repair
experiments
using
showed
remarkable
increase
local
quantity.
These
findings
suggest
SilMA
compositing
PRP
shows
great
potential
modulating
function.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(30)
Published: April 20, 2024
Abstract
Addressing
large
bone
defects
remains
a
significant
challenge
owing
to
the
inherent
limitations
in
self‐healing
capabilities,
resulting
prolonged
recovery
and
suboptimal
regeneration.
Although
current
clinical
solutions
are
available,
they
have
notable
shortcomings,
necessitating
more
efficacious
approaches
Organoids
derived
from
stem
cells
show
great
potential
this
field;
however,
development
of
organoids
has
been
hindered
by
specific
demands,
including
need
for
robust
mechanical
support
provided
scaffolds
hybrid
extracellular
matrices
(ECM).
In
context,
bioprinting
technologies
emerged
as
powerful
means
replicating
complex
architecture
tissue.
The
research
focused
on
fabrication
highly
intricate
ECM
analog
using
novel
bioink
composed
gelatin
methacrylate/alginate
methacrylate/hydroxyapatite
(GelMA/AlgMA/HAP).
Bioprinted
facilitate
long‐term
cultivation
progressive
maturation
extensive
bioprinted
organoids,
foster
multicellular
differentiation,
offer
valuable
insights
into
initial
stages
formation.
intrinsic
self‐mineralizing
quality
closely
emulates
properties
natural
bone,
empowering
with
enhanced
repair
both
vitro
vivo
applications.
This
trailblazing
investigation
propels
field
tissue
engineering
holds
promise
its
translation
practical
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
35(52)
Published: April 23, 2023
Advances
in
bioprinting
have
enabled
the
fabrication
of
complex
tissue
constructs
with
high
speed
and
resolution.
However,
there
remains
significant
structural
biological
complexity
within
tissues
that
is
unable
to
recapitulate.
Bone,
for
example,
has
a
hierarchical
organization
ranging
from
molecular
whole
organ
level.
Current
techniques
materials
employed
imposed
limits
on
scale,
speed,
resolution
can
be
achieved,
rendering
technique
reproduce
hierarchies
cell-matrix
interactions
are
observed
bone.
The
shift
toward
biomimetic
approaches
bone
engineering,
where
hydrogels
provide
biophysical
biochemical
cues
encapsulated
cells,
promising
approach
enhancing
function
development
vitro
modeling.
A
major
focus
modeling
creating
dynamic
microenvironmental
niches
support,
stimulate,
direct
cellular
processes
formation
remodeling.
Hydrogels
ideal
imitating
extracellular
matrix
since
they
engineered
present
various
whilst
allowing
bioprinting.
Here,
recent
advances
3D
niche
conducive
engineering
models
reviewed.
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
31, P. 101502 - 101502
Published: Jan. 19, 2025
DNA-based
hydrogels
stand
out
for
bone
regeneration
due
to
their
exceptional
biocompatibility
and
programmability.
These
facilitate
the
formation
of
spatial
structures
through
bulk
hydrogel
fabricating,
microsphere
formatting,
3D
printing.
Furthermore,
microenvironment
can
be
finely
tuned
by
leveraging
degradation
products,
nanostructure,
targeting,
delivery
capabilities
inherent
materials.
In
this
review,
we
underscore
advantages
hydrogels,
detailing
composition,
gelation
techniques,
structure
optimization.
We
then
delineate
three
critical
elements
in
promotion
using
hydrogels:
(i)
osteogenesis
driven
phosphate
ions,
plasmids,
oligodeoxynucleotides
(ODNs)
that
enhance
mineralization
promote
gene
protein
expression;
(ii)
vascularization
facilitated
tetrahedral
DNA
nanostructures
(TDNs)
aptamers,
which
boosts
expression
targeted
release;
(iii)
immunomodulation
achieved
loaded
factors,
TDNs,
bound
ions
stimulate
macrophage
polarization
exhibit
antibacterial
properties.
With
these
properties,
used
construct
organoids,
providing
an
innovative
tool
disease
modeling
therapeutic
applications
tissue
engineering.
Finally,
discuss
current
challenges
future
prospects,
emphasizing
potential
impacts
regenerative
medicine.
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
31, P. 101509 - 101509
Published: Jan. 22, 2025
Articular
cartilage,
composed
of
chondrocytes
within
a
dynamic
viscoelastic
matrix,
has
limited
self-repair
capacity,
posing
significant
challenge
for
regeneration.
Constructing
high-fidelity
cartilage
organoids
through
three-dimensional
(3D)
bioprinting
to
replicate
the
structure
and
physiological
functions
is
crucial
regenerative
medicine,
drug
screening,
disease
modeling.
However,
commonly
used
matrix
bioinks
lack
reversible
cross-linking
precise
controllability,
hindering
cellular
regulation.
Thus,
encoding
adaptive
cultivating
an
attractive
idea.
DNA,
with
its
ability
be
intricately
encoded
reversibly
cross-linked
into
hydrogels,
offers
manipulation
at
both
molecular
spatial
structural
levels.
This
endows
hydrogels
viscoelasticity,
printability,
cell
recognition,
stimuli
responsiveness.
paper
elaborates
on
strategies
encode
bioink
via
emphasizing
regulation
predictable
properties
resulting
interactions
behavior.
The
significance
these
construction
highlighted.
Finally,
we
discuss
challenges
future
prospects
using
DNA-encoded
3D
bioprinted
organoids,
underscoring
their
potential
impact
advancing
biomedical
applications.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(29)
Published: March 14, 2024
Abstract
Aqueous
two‐phase
emulsion
(ATPE)‐based
bioinks,
a
creative
innovation
for
bioprinting,
enable
the
fabrication
of
complex
3D
cell‐laden
hydrogels
with
macroporous
structure,
which
promote
cellular
activities
within
scaffold.
However,
these
bioinks
intrinsically
lack
stability
and
specific
biofunctionality,
potentially
limiting
their
application
targeted
tissue
engineering.
This
study
proposes
new
perspective
by
introducing
less
than
0.1%
phosphorylated
cellulose
nanofibrils
(pCNF),
1D
nanofibril
top‐down
produced
from
natural
biomasses,
into
dextran/methacrylated
gelatin
(GelMA)‐based
ATPE
system
extrusion‐based
bioprinting
preosteoblastic
cells,
aiming
to
fabricate
osteogenic
differentiation
potential.
The
pCNF
that
is
selectively
partitioned
in
GelMA
phase
can
not
only
improve
alter
rheological
behaviors
ATPE‐based
bioink,
but
also
enhance
damping
capacity
mineralization
ability
crosslinked
hydrogels.
Furthermore,
demonstrate
increased
cell
activity
higher
viability
post‐printing,
along
alkaline
phosphatase
osteoblastic
gene
expression.
Importantly,
organized
interfaces
hydrogel
facilitate
formation
macroscopic
biomineralized
nodules
vitro.
incorporation
multifunctional
significantly
boosts
physiochemical
biological
performance
macropore‐forming
transforming
them
suitable
platform
engineering
vitro
bone
models.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 25, 2024
Abstract
Traditional
hydrogels
often
face
issues
like
dehydration,
excessive
swelling,
and
poor
adhesion,
limiting
their
practical
applications.
This
study
presents
a
facile
universal
method
to
create
elastomer‐encapsulated
with
improved
water
retention,
non‐swelling,
enhanced
adhesion.
n‐Butyl
acrylate
(BA)
2,2,3,4,4,4‐hexafluorobutyl
methacrylate
(HFBMA)
are
utilized
as
the
“soft”
“hard”
monomers,
respectively,
in
situ
construct
elastomer
coatings
on
hydrogel
surface
through
surface‐confined
copolymerization.
The
resulting
transparent,
hydrophobic,
adhesive
coating
is
tightly
bound
surface,
conferring
upon
it
robust
defense
against
dehydration
swelling
various
media,
strong
adhesion
diverse
substrates
both
aerial
submerged
conditions.
Furthermore,
this
encapsulation
strategy
also
augments
mechanical
attributes
of
bulk
hydrogel,
including
its
tensile
properties
puncture
resistance,
applicable
wide
array
types
configurations.
Additionally,
applied
conductive
results
flexible
sensors
high
sensitivity,
reversible
resistance
change,
exceptional
sensing
stability,
significantly
durability
air
underwater
environments.
These
suggest
potential
applications
harsh
environments,
such
acoustic
detection
sonar
scanning
camouflage
for
submarines.
