Maize TIR-only Proteins ZmTIR1 and ZmTIR2, but not ZmTIR3 Confer Auto-active Hypersensitive Response Likely by Forming Condensation DOI Creative Commons
Zhen Kang, Qidong Ge,

M. Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

SUMMARY Nucleotide binding, leucine-rich-repeat (NLR) proteins are the major intracellular receptors for defending against pathogen infection. The recognition between NLRs and secreted effectors often triggers a localized programmed cell death termed hypersensitive response (HR). Despite significant progresses have been achieved in understanding canonical with N-terminal Toll/interleukin-1 receptor (TIR) domains, molecular mechanisms underlying TIR-only plant immune responses remain unclear. In this study, we identified six TIR-containing maize, including three proteins. Functional analysis showed that ZmTIR1 ZmTIR2, but not ZmTIR3, confer autoactive HR when transiently expressed N. benthamiana . autoactivity conferred by ZmTIR2 depends on EDS1-PAD4-RNL module their putative NADase activities. Interestingly, predominantly localize punctate dots likely form condensation, while ZmTIR3 mainly localizes cytoplasm nucleus. Two specific amino acids BB-loop region were to be required ZmTIR1- ZmTIR2-mediated condensation formation auto-HR. Furthermore, ZmTIR induced Cochliobolus heterostrophus , causal agent of southern leaf blight (SLB) knock-down expression or decreased resistance SLB maize. Our study reveals novel mechanism monocot maize responses.

Language: Английский

A canonical protein complex controls immune homeostasis and multipathogen resistance DOI

Yue Wu,

Weiying Xu, Guoyan Zhao

et al.

Science, Journal Year: 2024, Volume and Issue: 386(6728), P. 1405 - 1412

Published: Nov. 7, 2024

The calcium (Ca

Language: Английский

Citations

10
Rational design of phytovirucide inhibiting nucleocapsid protein aggregation in tomato spotted wilt virus DOI Creative Commons
Ningning Zan, Jiao Li, Jiahui Yao

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 27, 2025

Ineffectiveness of managing plant viruses by chemicals has posed serious challenges in crop production. Recently, phase separation shown to play a key role viral lifecycle. Using inhibitors that can disturb biomolecular condensates formed for virus control been reported medical field. However, the applicability this promising antiviral tactic protection not explored. Here, we report an inhibitor, Z9, targets tomato spotted wilt (TSWV) N protein. Z9 is capable interacting with amino acids nucleic acid binding region TSWV N, disrupting assembly and RNA into phase-separated condensates, reduction which detrimental stability This study provides strategy separation-based control. Biomolecular have recognized as crucial process life cycle. authors inhibitor targeting nucleocapsid protein (N) disrupt its potential

Language: Английский

Citations

1
Exploring the frontier of plant phase separation: Current insights and future prospects DOI Creative Commons

Panting Fan,

Jingjing Zhang, Lefei Gao

et al.

New Crops, Journal Year: 2024, Volume and Issue: 1, P. 100026 - 100026

Published: May 16, 2024

In the rapidly evolving field of biology, phase separation has recently emerged as a revolutionary perspective, shedding new light on our comprehension cellular processes. This review provides comprehensive overview current knowledge regarding in plants and charts promising avenues for future exploration. We delve into fundamental principles plant separation, highlighting roles played by intrinsically disordered regions prion-like domains. Summarizing significant advancements, we explore involvement responses to environmental cues, well its growth developmental processes, plant-microbe interactions. Additionally, present streamlined workflow designed guide scientific community conducting studies plants. Lastly, delineate lingering questions propose potential applications agriculture.

Language: Английский

Citations

4
Navigating biomolecular condensates in plants from patterns to functions DOI
Yansong Miao, Monika Chodasiewicz, Xiaofeng Fang

et al.

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(9), P. 1329 - 1332

Published: Aug. 14, 2024

Language: Английский

Citations

4
Assembly and functional mechanisms of plant NLR resistosomes DOI
Shijia Huang, Ertong Li, Fangshuai Jia

et al.

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 90, P. 102977 - 102977

Published: Jan. 13, 2025

Language: Английский

Citations

0
Białka ulegające separacji faz u roślin i metody ich badań DOI Open Access
Marta Gapińska

Postępy Biochemii, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Numerous scientific reports in recent years have indicated that liquid-liquid phase separation (LLPS) is an important mechanism allowing plants to identify various biotic and abiotic stresses respond them. The following paper reviews the methods currently used study macromolecular condensates function of proteins undergoing LLPS plants.

Language: Английский

Citations

0
Downstream signaling induced by several plant Toll/interleukin-1 receptor-containing immune proteins is stable at elevated temperature DOI Creative Commons

Héloïse Demont,

Céline Remblière,

Raphaël Culerrier

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115326 - 115326

Published: Feb. 19, 2025

Language: Английский

Citations

0
Bacillus velezensis YN2111 reveals the potential role of Amine Oxidase in Fusarium Wilt response in Cavendish bananas DOI

Hongwei Yu,

Guangdong Zhou, Wenlong Zhang

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102653 - 102653

Published: March 1, 2025

Language: Английский

Citations

0
Functional analysis of AtTX11/12 TIR-domain proteins identifies key residues for basal and temperature-insensitive growth inhibition DOI

Sang‐Min Lim,

Yo Han Kim,

Young Bin Yun

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 749, P. 151357 - 151357

Published: Jan. 18, 2025

Language: Английский

Citations

0
Switch of TIR signaling by a Ca2+ sensor activates ADR1 recognition of pRib-AMP-EDS1-PAD4 for stomatal immunity DOI Creative Commons
Hanling Wang,

Jiaxin Tan,

Xiulin Cui

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 2, 2024

Abstract Plants swiftly close stomata upon detecting pathogen entry, a crucial defense termed stomatal immunity. The process is initiated by cell-surface pattern recognition receptors (PRRs) that perceive pathogen-associated molecular patterns (PAMPs) and evoke series of early cellular responses including calcium ions (Ca 2+ ) influx, conducted the intracellular nucleotide-binding leucine-rich-repeat (NLRs) ADR1s within an EDS1-PAD4-ADR1 module. However, underlying mechanisms linking PRR signaling to NLRs remain unclear. Here, we show Nicotiana benthamiana Toll/interleukin-1 receptor (TIR)-only protein Stomatal TIR1 (STIR1) produces immune molecule pRib-AMP, induces formation complexes, mediates Inhibitor Immunity C2-domain 1 (ISIC1) interacts with constrains STIR1 function at basal condition, whereas infection, ISIC1 senses Ca signals de-represses signaling. Cryo-electron microscopy structure infection-elicited Arabidopsis AtEDS1-AtPAD4-AtADR1-L2 complex reveals pRib-AMP binding AtEDS1-AtPAD4 AtADR1-L2 pRib-AMP-AtPAD4-AtEDS1 for Collectively, this study uncovers repression/de-repression mechanism sensor/TIR-only node, elucidates NLR pRib-AMP-EDS1-PAD4 in governing innate Synopsis At sensor inhibits TIR-only STIR1; perceives signal releases produce pRib-AMP; EDS1-PAD4 binds recognized ADR1-L2, thereby activating

Language: Английский

Citations

2