The Chromosome-Level Genome Sequence of the Autotetraploid Alfalfa and Resequencing of Core Germplasms Provide Genomic Resources for Alfalfa Research

Molecular Plant, Journal Year: 2020, Volume and Issue: 13(9), P. 1250 - 1261

Published: July 13, 2020

Language: Английский

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The Current Situation of Pea Protein and Its Application in the Food Industry

Molecules, Journal Year: 2022, Volume and Issue: 27(16), P. 5354 - 5354

Published: Aug. 22, 2022

Pea (

Language: Английский

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Improved pea reference genome and pan-genome highlight genomic features and evolutionary characteristics

Nature Genetics, Journal Year: 2022, Volume and Issue: 54(10), P. 1553 - 1563

Published: Sept. 22, 2022

Abstract Complete and accurate reference genomes annotations provide fundamental resources for functional genomics crop breeding. Here we report a de novo assembly annotation of pea cultivar ZW6 with contig N50 8.98 Mb, which features 243-fold increase in length evident improvements the continuity quality sequence complex repeat regions compared existing one. Genome diversity 118 cultivated wild demonstrated that Pisum abyssinicum is separate species different from P. fulvum sativum within . Quantitative trait locus analyses uncovered two known Mendel’s genes related to stem ( Le/le ) seed shape R/r as well some candidate pod form studied by Mendel. A pan-genome 116 accessions was constructed, pan-genes preferred showed distinct enrichment, indicating potential value them breeding future.

Language: Английский

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High-quality genome sequence of white lupin provides insight into soil exploration and seed quality

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Jan. 24, 2020

White lupin (Lupinus albus L.) is an annual crop cultivated for its protein-rich seeds. It adapted to poor soils due the production of cluster roots, which are made dozens determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence accession white (2n = 50, 451 Mb), as well de novo assemblies landrace wild relative. We describe modern displaying increased capacity through early establishment roots. also show how seed quality may have been impacted by domestication in term protein profiles alkaloid content. The availability assembly together with companion genomic transcriptomic resources will enable development breeding strategies increase stabilize yield.

Language: Английский

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Potential and limits of exploitation of crop wild relatives for pea, lentil, and chickpea improvement

Legume Science, Journal Year: 2020, Volume and Issue: 2(2)

Published: April 7, 2020

Abstract Legumes represent the second most important family of crop plants after grasses, accounting for approximately 27% world's production. Past domestication processes resulted in a high degree relatedness between modern varieties crops, leading to narrower genetic base cultivated germplasm prone pests and diseases. Crop wild relatives (CWRs) harbor diversity tested by natural selection range environments. To fully understand exploit local adaptation CWR, studies geographical centers origin combining ecology, physiology, genetics are needed. With advent genomics computation, combined with systematic phenotyping, it is feasible revisit accessions landraces prioritize their use breeding, providing sources disease resistances; tolerances drought, heat, frost, salinity abiotic stresses; nutrient densities across major minor elements; food quality traits. Establishment hybrid populations CWRs gives breeders considerable benefit prebreeding tool identifying harnessing alleles provides extremely valuable long‐term resources. There need further collecting both ex situ conservation CWR these taxa face habitat loss degradation climate change. In this review, we focus on three legume crops domesticated Fertile Crescent, pea, chickpea, lentil, summarize current state potential respective improvement.

Language: Английский

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Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)

Frontiers in Plant Science, Journal Year: 2019, Volume and Issue: 10

Published: Nov. 26, 2019

Genome-wide association study (GWAS) was completed to identify loci associated with agronomic (days flowering, days maturity, plant height, seed yield and weight), morphology (shape dimpling) quality (protein, starch fibre concentrations) traits of field pea (Pisum sativum L.). A collection 135 accessions from 23 different breeding programs in Africa (Ethiopia), Asia (India), Australia, Europe (Belarus, Czech Republic, Denmark, France, Lithuania, Netherlands, Russia, Sweden, Ukraine United Kingdom) North America (Canada USA), used for the GWAS. The were genotyped using genotyping-by-sequencing (GBS). After filtering a minimum read depth five, minor allele frequency 0.05, 16,877 high SNPs selected determine marker-trait associations (MTA). LD decay (LD1/2max, 90) across chromosomes varied 20 80 kb. Population structure analysis grouped into nine subpopulations. evaluated multi-year, multi-location trials Olomouc (Czech Republic), Fargo, Dakota (USA), Rosthern Saskatoon, Saskatchewan (Canada) 2013 2017. Each trait phenotyped at least five location-years. MTAs that consistent multiple identified. Chr5LG3_566189651 Chr5LG3_572899434 Chr2LG1_409403647 lodging resistance, Chr1LG6_57305683 Chr1LG6_366513463 grain yield, Chr1LG6_176606388, Chr2LG1_457185, Chr3LG5_234519042 Chr7LG7_8229439 concentration, Chr3LG5_194530376 protein concentration identified locations years. This research SNP markers important are potential marker-assisted selection towards rapid cultivar improvement.

Language: Английский

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The genome evolution and low-phosphorus adaptation in white lupin

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Feb. 26, 2020

Abstract White lupin ( Lupinus albus ) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils. Here, we assemble the genome of white find it evolved from whole-genome triplication (WGT) event. We then decipher its diploid ancestral reconstruct three sub-genomes. Based on results, further reveal sub-genome dominance genic expression different sub-genomes varying relation to their transposable element (TE) density. The PUE genes have been expanded through WGT as well tandem dispersed duplications. Furthermore, characterize four main pathways for PUE, which include carbon fixation, root formation, soil-P remobilization, cellular-P reuse. Among these, auxin modulation may be important formation involvement potential LaABCG36 s LaABCG37 s. These findings provide insights into evolution adaptation lupin.

Language: Английский

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CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges

Legume Science, Journal Year: 2021, Volume and Issue: 3(3)

Published: May 11, 2021

Abstract Legumes are an excellent source of proteins and health‐promoting phytochemicals. Recognizing their importance in human nutrition sustainable agricultural production, significant efforts currently being made to accelerate genetic gain related yield, stress resilience, nutritional quality. Recent increases genomic resources for multiple legume crops have laid a solid foundation application transformative breeding technologies such as selection genome editing crop improvement. In this review, we focus on the recent plant‐specific advances CRISPR/Cas9‐based gene technology discuss challenges opportunities harnessing innovative targeted improvement traits crops. Gene‐editing methods been successfully established soybean, cowpea, chickpea, model legumes Medicago truncatula Lotus japonicus . However, recalcitrance other vitro transfer regeneration has posed serious challenge editing. We various modifications culture methods, terms choice explant, media composition, DNA delivery gene‐editing detection that can potentially improve rate transformation whole plant Although bring enormous benefits breeding, regulatory hurdles cause concern. compare environments existing European Union United States America. A favorable framework public acceptance important factors realizing CRISPR's potential global food security.

Language: Английский

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Advancing crop genomics from lab to field

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(5), P. 595 - 601

Published: May 1, 2021

Language: Английский

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MtExpress, a Comprehensive and Curated RNAseq-based Gene Expression Atlas for the Model Legume Medicago truncatula

Plant and Cell Physiology, Journal Year: 2021, Volume and Issue: 62(9), P. 1494 - 1500

Published: July 10, 2021

ABSTRACT Although RNA sequencing (RNAseq) has been becoming the main transcriptomic approach in model legume Medicago truncatula, there is currently no genome-wide gene expression atlas covering whole set of RNAseq data published for this species. Nowadays, such a tool highly valuable to provide global view wide range conditions and tissues/organs. Here, we present MtExpress, that compiles an exhaustive M. truncatula (https://medicago.toulouse.inrae.fr/MtExpress). MtExpress makes use recent releases genome sequence annotation, as well up-to-date tools perform mapping, quality control, statistical analysis normalization data. combines semi-automated pipelines with manual re-labeling organization samples produce attractive user-friendly interface, fully integrated other available genomic resources. Importantly, flexible, terms both queries, e.g. allowing searches names orthologous IDs from Arabidopsis species, outputs, customize visualization redirect study relevant webservers. Thanks its pipeline, will be frequently updated follow rapid pace publications, constant improvement annotation. also hosts legacy GeneChip originally stored Gene Expression Atlas, very complementary resource.

Language: Английский

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