Rapid measurement of SARS-CoV-2 spike T cells in whole blood from vaccinated and naturally infected individuals

Journal of Clinical Investigation, Journal Year: 2021, Volume and Issue: 131(17)

Published: Aug. 31, 2021

Defining the correlates of protection necessary to manage COVID-19 pandemic requires analysis both antibody and T cell parameters, but complexity traditional tests limits virus-specific measurements. We tested sensitivity performance a simple rapid SARS-CoV-2 spike protein–specific test based on stimulation whole blood with peptides covering protein, followed by cytokine (IFN-γ, IL-2) measurement in different cohorts including BNT162b2-vaccinated individuals (n = 112), convalescent asymptomatic symptomatic patients 130), SARS-CoV-1–convalescent 12). The this is comparable that methods (ELISPOT, activation-induced marker). Using test, we observed similar mean magnitude responses between vaccinees convalescents 3 months after vaccination or virus priming. However, wide heterogeneity spike-specific characterized individual responses, irrespective time analysis. these cannot be predicted from neutralizing levels. Hence, humoral cellular spike–specific immunity should define evaluate current vaccine strategies.

Language: Английский

---

Robust T-Cell Responses in Anti-CD20-Treated Patients Following COVID-19 Vaccination: A Prospective Cohort Study

Clinical Infectious Diseases, Journal Year: 2021, Volume and Issue: 75(1), P. e1037 - e1045

Published: Nov. 15, 2021

Abstract Background Patients treated with anti-CD20 therapy are particularly at risk of developing severe coronavirus disease 2019 (COVID-19); however, little is known regarding COVID-19 vaccine effectiveness in this population. Methods This prospective observational cohort study assesses humoral and T-cell responses after vaccination 2 doses mRNA-based vaccines patients rituximab for rheumatic diseases or ocrelizumab multiple sclerosis (n = 37), compared to immunocompetent individuals 22). Results Severe acute respiratory syndrome (SARS-CoV-2)-specific antibodies were detectable only 69.4% levels that significantly lower controls who all seroconverted. In contrast antibodies, Spike (S)-specific CD4 T cells equally detected (85–90%) mostly a Th1 phenotype. Response rates S-specific CD8 higher (96.2%) rituximab-treated (81.8%) as (66.7%). polyfunctional but expressed more effector molecules than controls. During follow-up, 3 MS without SARS-CoV-2-specific antibody response had mild breakthrough infection. One them no vaccination. Conclusions Our suggests on treatment able mount potent mRNA vaccines, despite impaired responses. could play an important role the reduction complications COVID-19.

Language: Английский

---

Efficacy of a third BNT162b2 mRNA COVID-19 vaccine dose in patients with CLL who failed standard 2-dose vaccination

Blood, Journal Year: 2021, Volume and Issue: 139(5), P. 678 - 685

Published: Dec. 3, 2021

Language: Английский

---

COVID-19 in patients with hematologic malignancy

Blood, Journal Year: 2022, Volume and Issue: 140(3), P. 236 - 252

Published: May 11, 2022

Language: Английский

---

Antibodies elicited by SARS-CoV-2 infection or mRNA vaccines have reduced neutralizing activity against Beta and Omicron pseudoviruses

Science Translational Medicine, Journal Year: 2022, Volume and Issue: 14(634)

Published: Jan. 14, 2022

Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that have mutations associated with increased transmission and antibody escape arisen over the course of current pandemic. Although vaccines largely been effective against past variants, number found on Omicron (B.1.1.529) spike protein appear to diminish protection conferred by preexisting immunity. Using vesicular stomatitis virus (VSV) pseudoparticles expressing several SARS-CoV-2 we evaluated magnitude breadth neutralizing response time in individuals after infection mRNA-vaccinated individuals. We observed boosting increases wild-type (D614), Beta, Delta, variants; however, variant was most resistant neutralization. further vaccinated healthy adults had robust broad responses, whereas responses may reduced pregnant women, underscoring importance learning how maximize mRNA vaccine populations. Findings from this study show substantial heterogeneity vaccination support addition more conserved viral antigens existing vaccines.

Language: Английский

---