ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(10), P. 3623 - 3642
Published: Oct. 11, 2023
Over
the
past
few
decades,
pathogens
have
posed
a
threat
to
human
security,
and
rapid
identification
of
should
be
one
ideal
methods
prevent
major
public
health
security
outbreaks.
Therefore,
there
is
an
urgent
need
for
highly
sensitive
specific
approaches
identify
quantify
pathogens.
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
CRISPR/Cas
systems
Argonaute
(Ago)
belong
Microbial
Defense
Systems
(MDS).
The
guided,
programmable,
targeted
activation
nucleases
by
both
them
leading
way
new
generation
detection.
We
compare
these
two
in
terms
similarities
differences.
In
addition,
we
discuss
future
challenges
prospects
development
biosensors,
especially
electrochemical
biosensors.
This
review
expected
afford
researchers
entering
this
multidisciplinary
field
useful
guidance
provide
inspiration
more
innovative
biosensors
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
13(7), P. 2877 - 2896
Published: Feb. 24, 2023
The
ongoing
coronavirus
disease
2019
(COVID-19)
pandemic
has
boosted
the
development
of
antiviral
research.
Microfluidic
technologies
offer
powerful
platforms
for
diagnosis
and
drug
discovery
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
discovery.
In
this
review,
we
introduce
structure
SARS-CoV-2
basic
knowledge
microfluidic
design.
We
discuss
application
devices
in
based
on
detecting
viral
nucleic
acid,
antibodies,
antigens.
highlight
contribution
lab-on-a-chip
to
manufacturing
point-of-care
equipment
accurate,
sensitive,
low-cost,
user-friendly
virus-detection
devices.
then
investigate
efforts
organ-on-a-chip
lipid
nanoparticles
(LNPs)
synthesizing
chips
screening
mRNA
vaccine
preparation.
contribute
research
provide
tools
future
outbreaks.
Annual Review of Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
16(1), P. 311 - 332
Published: April 5, 2023
Biosensing
based
on
CRISPR-Cas
systems
is
a
young
but
rapidly
evolving
technology.
The
unprecedented
properties
of
the
system
provide
an
innovative
tool
for
developing
new-generation
biosensing
strategies.
To
date,
series
nucleic
acid
and
non-nucleic
detection
methods
have
been
developed
CRISPR
platform.
In
this
review,
we
first
introduce
core
biochemical
underpinning
development
bioassays,
such
as
diverse
reaction
temperatures,
programmability
in
design,
high
efficiency,
recognition
specificity,
highlight
recent
efforts
to
improve
these
parameters.
We
then
technical
developments,
including
how
sensitivity
quantification
capabilities,
develop
multiplex
assays,
achieve
convenient
one-pot
create
advanced
sensors,
extend
applications
detection.
Finally,
analyze
obstacles
commercial
application
technology
explore
opportunities
directions.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(10), P. 3623 - 3642
Published: Oct. 11, 2023
Over
the
past
few
decades,
pathogens
have
posed
a
threat
to
human
security,
and
rapid
identification
of
should
be
one
ideal
methods
prevent
major
public
health
security
outbreaks.
Therefore,
there
is
an
urgent
need
for
highly
sensitive
specific
approaches
identify
quantify
pathogens.
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
CRISPR/Cas
systems
Argonaute
(Ago)
belong
Microbial
Defense
Systems
(MDS).
The
guided,
programmable,
targeted
activation
nucleases
by
both
them
leading
way
new
generation
detection.
We
compare
these
two
in
terms
similarities
differences.
In
addition,
we
discuss
future
challenges
prospects
development
biosensors,
especially
electrochemical
biosensors.
This
review
expected
afford
researchers
entering
this
multidisciplinary
field
useful
guidance
provide
inspiration
more
innovative
biosensors
