The
rapid
and
accurate
detection
of
parasites
is
crucial
for
timely
curative
intervention
in
parasitosis
epidemiological
surveillance.
To
meet
the
needs
clinical
diagnosis,
it
imperative
to
develop
a
diagnostic
tool
based
on
nucleic
acid
that
combines
sensitivity
specificity
established
amplifica-tion
tests
with
speed,
cost-effectiveness,
convenience
isothermal
amplification
methods.
A
new
nu-cleic
method,
utilizing
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-associated
(Cas)
nuclease,
holds
promise
point-of-care
testing.
Specifically,
CRISPR-Cas12a
system
has
demonstrated
numerous
advantages
detecting
parasites,
hopeful
outcomes
identify-ing
malaria,
toxoplasmosis,
other
parasitic
ailments.
This
review
provides
an
overview
how
can
be
utilized
parasite
detection,
evaluates
its
disadvantages,
sug-gests
ways
improve
efficiency
CRISPR-Cas12a-based
assays.
Sensors and Actuators A Physical,
Journal Year:
2024,
Volume and Issue:
376, P. 115625 - 115625
Published: June 24, 2024
The
integration
of
CRISPR
technology
with
microfluidic-based
biosensors
has
greatly
expanded
its
applications
in
medicine
and
molecular
biology.
This
combination
offers
enhanced
sensitivity
selectivity
medical
diagnostics.
CRISPR-powered
microfluidics
enables
the
accurate
quantification
DNA
RNA
associated
diseases
such
as
cancer,
viral
infections,
bacterial
diseases.
precise
targeting
capabilities
allow
for
detection
specific
genetic
sequences,
aiding
early
diagnosis,
disease
monitoring,
treatment
assessment.
improves
specificity
by
leveraging
programmable
nature
coupling
it
signal
readouts.
However,
challenges
assay
optimization,
standardization,
device
fabrication
need
to
be
addressed.
Additionally,
complexity
presents
potential
limitations
terms
off-target
effects
unintended
modifications.
Overall,
holds
tremendous
diagnostics,
but
further
research
development
are
required
fully
exploit
benefits
clinical
settings.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(6), P. 2676 - 2683
Published: Jan. 30, 2024
Sepsis
is
an
extremely
dangerous
medical
condition
that
emanates
from
the
body's
response
to
a
pre-existing
infection.
Early
detection
of
sepsis-inducing
bacterial
infections
can
greatly
enhance
treatment
process
and
potentially
prevent
onset
sepsis.
However,
current
point-of-care
(POC)
sensors
are
often
complex
costly
or
lack
ideal
sensitivity
for
effective
detection.
Therefore,
it
crucial
develop
rapid
sensitive
biosensors
on-site
bacteria.
Herein,
we
developed
graphene
oxide
CRISPR-Cas12a
(GO-CRISPR)
biosensor
bacteria
in
human
serum.
In
this
strategy,
single-stranded
(ssDNA)
FAM
probes
were
quenched
with
single-layer
(GO).
Target-activated
Cas12a
trans-cleavage
was
utilized
degradation
ssDNA
probes,
detaching
short
GO
recovering
fluorescent
signals.
Under
optimal
conditions,
employed
our
GO-CRISPR
system
Salmonella
Typhimurium
(S.
Typhimurium)
as
low
3
×
103
CFU/mL
serum,
well
good
specificity
toward
other
competing
addition,
exhibited
excellent
S.
spiked
The
offers
superior
rapidity
has
potential
early
resource-limited
settings,
expediting
patients
at
risk
Critical Reviews in Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 25
Published: Sept. 17, 2024
This
comprehensive
review
delves
into
the
advancements
and
challenges
in
biosensing,
with
a
strong
emphasis
on
transformative
potential
of
CRISPR
technology
for
early
rapid
detection
infectious
diseases.
It
underscores
versatility
CRISPR/Cas
systems,
highlighting
their
ability
to
detect
both
nucleic
acids
non-nucleic
acid
targets,
seamless
integration
isothermal
amplification
techniques.
The
provides
thorough
examination
latest
developments
CRISPR-based
biosensors,
detailing
unique
properties
such
as
high
specificity
programmability,
which
make
them
particularly
effective
detecting
disease-associated
acids.
While
focuses
due
its
critical
role
diagnosing
diseases,
it
also
explores
broader
applications
thereby
acknowledging
technology's
potential.
Additionally,
identifies
existing
challenges,
need
improved
signal
real-world
applicability,
offers
future
perspectives
aimed
at
overcoming
these
hurdles.
ultimate
goal
is
advance
development
highly
sensitive
specific
biosensors
that
can
be
used
widely
improving
human
health,
point-of-care
settings
resource-limited
environments.
Annual Review of Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
17(1), P. 459 - 474
Published: Feb. 16, 2024
Nucleic
acid
testing
is
the
cornerstone
of
modern
molecular
diagnostics.
This
review
describes
current
status
and
future
directions
diagnostics,
focusing
on
four
major
techniques:
polymerase
chain
reaction
(PCR),
next-generation
sequencing
(NGS),
isothermal
amplification
methods
such
as
recombinase
(RPA)
loop-mediated
(LAMP),
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
detection
methods.
We
explore
advantages
limitations
each
technique,
describe
how
overlaps
with
or
complements
other
techniques,
examine
clinical
offerings.
provides
a
broad
perspective
into
landscape
diagnostics
highlights
potential
in
this
rapidly
evolving
field.
