Single-Cell Stretching in Viscoelastic Fluids with Electronically Triggered Imaging for Cellular Mechanical Phenotyping DOI
Minhui Liang, Dahou Yang, Yinning Zhou

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(10), P. 4567 - 4575

Published: March 4, 2021

Cellular mechanical phenotypes in connection to physiological and pathological states of cells have become a promising intrinsic biomarker for label-free cell analysis various biological research medical diagnostics. In this work, we present microfluidic system capable high-throughput cellular phenotyping based on rapid single-cell hydrodynamic stretching continuous viscoelastic fluid flow. Randomly introduced single are first aligned into streamline fluids before being guided flow splitting junction consistent stretching. The arrival individual prior the can be detected by an electrical sensing unit, which produces triggering signal activate high-speed camera on-demand imaging motion deformation through junction. phenotypes, including size deformability, extracted from these captured images. We evaluated sensitivity developed measuring synthesized hydrogel microbeads with known Young's modulus. With system, revealed statistical difference deformability microfilament disrupted, normal, fixed NIH 3T3 fibroblast cells. Furthermore, implementation machine-learning-based classification MCF-10A MDA-MB-231 mixtures, our has achieved comparable accuracy (0.9:1, 5.03:1) respect fluorescence-based cytometry results (0.97:1, 5.33:1). presented technique will open new avenues diverse biomedical applications.

Language: Английский

Mechanical properties of single cells: Measurement methods and applications DOI
Yansheng Hao, Shaokoon Cheng, Yo Tanaka

et al.

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 45, P. 107648 - 107648

Published: Oct. 17, 2020

Language: Английский

Citations

82
Microfluidic technologies for cell deformability cytometry DOI Creative Commons
Hanxu Chen, Jiahui Guo, Feika Bian

et al.

Smart Medicine, Journal Year: 2022, Volume and Issue: 1(1)

Published: Dec. 1, 2022

Microfluidic detection methods for cell deformability cytometry have been regarded as powerful tools single-cell analysis of cellular mechanical phenotypes, thus having widely applied in the fields preparation, separation, clinical diagnostics and so on. Featured with traits like easy operations, low cost high throughput, such shown great potentials on investigating physiological state pathological changes during deformation. Herein, a review advancements microfluidic-based deformation is presented. We discuss several representative their frontiers practical applications. Finally, we analyze current status propose remaining challenges future perspectives development directions.

Language: Английский

Citations

56
Microfluidic Impedance‐Deformability Cytometry for Label‐Free Single Neutrophil Mechanophenotyping DOI
Chayakorn Petchakup, Haoning Yang, Lingyan Gong

et al.

Small, Journal Year: 2022, Volume and Issue: 18(18)

Published: March 7, 2022

Abstract The intrinsic biophysical states of neutrophils are associated with immune dysfunctions in diseases. While advanced image‐based flow cytometers can probe cell deformability at high throughput, it is nontrivial to couple different sensing modalities (e.g., electrical) measure other critical attributes including viability and membrane integrity. Herein, an “optics‐free” impedance‐deformability cytometer for multiparametric single mechanophenotyping reported. microfluidic platform integrates hydrodynamic pinching, multifrequency impedance quantification size, deformability, (indicative activation). A newly‐defined “electrical index” validated by numerical simulations, shows strong correlations the optical index HL‐60 experimentally. Human treated various biochemical stimul further profiled, distinct differences multimodal signatures UMAP analysis observed. Overall, integrated enables label‐free profiling throughput >1000 cells min −1 without any antibodies labeling facilitate clinical diagnostics.

Language: Английский

Citations

53
Viscoelastic properties of suspended cells measured with shear flow deformation cytometry DOI Creative Commons
Richard Gerum,

Elham Mirzahossein,

Mar Eroles

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Sept. 2, 2022

Numerous cell functions are accompanied by phenotypic changes in viscoelastic properties, and measuring them can help elucidate higher level cellular health disease. We present a high-throughput, simple low-cost microfluidic method for quantitatively the elastic (storage) viscous (loss) modulus of individual cells. Cells suspended high-viscosity fluid pumped with high pressure through 5.8 cm long 200 µm wide channel. The shear stress induces large, ear ellipsoidal deformations. In addition, flow profile channel causes cells to rotate tank-treading manner. From deformation tank treading frequency, we extract frequency-dependent properties based on theoretical framework developed R. Roscoe [1] that describes sphere under steady laminar flow. confirm accuracy using atomic force microscopy-calibrated polyacrylamide beads Our measurements demonstrate exhibit power-law, soft glassy rheological behavior is cell-cycle-dependent mediated physical interplay between actin filament intermediate networks.Cells human body viscoelastic: they have some an solid, like rubber, as well fluid, oil. To carry out mechanical tasks – such as, migrating tissues heal wound or fight inflammation need right balance viscosity elasticity. Measuring these two therefore researchers understand important how impacted However, quantifying tricky, both depend time-scale measured: when pressed slowly, appear liquid, but turn hard thick rapidly pressed. Here, Gerum et al. new system elasticity fast, simple, inexpensive. this method, specialized solution consistency similar machine oil which then pushed channels less than half millimeter wide. resulting shears cells, causing elongate rotate, captured fast camera takes 500 images per second. used artificial intelligence each cell’s shape rotation speed from images, calculated their existing theories objects behave fluids. also investigated changed frequencies, corresponds shorter time-scales. This revealed while frequencies made more elastic, ratio remained same. means compare results obtained different experimental techniques, even if were carried at completely provides inexpensive way analyzing It could be useful tool screening effects drugs, diagnostic detect diseases affect

Language: Английский

Citations

42
Computer vision meets microfluidics: a label-free method for high-throughput cell analysis DOI Creative Commons
Shizheng Zhou, Bingbing Chen,

Edgar S. Fu

et al.

Microsystems & Nanoengineering, Journal Year: 2023, Volume and Issue: 9(1)

Published: Sept. 21, 2023

In this paper, we review the integration of microfluidic chips and computer vision, which has great potential to advance research in life sciences biology, particularly analysis cell imaging data. Microfluidic enable generation large amounts visual data at single-cell level, while vision techniques can rapidly process analyze these extract valuable information about cellular health function. One key advantages integrative approach is that it allows for noninvasive low-damage characterization, important studying delicate or fragile microbial cells. The use provides a highly controlled environment growth manipulation, minimizes experimental variability improves accuracy analysis. Computer be used recognize target species within heterogeneous populations, understanding physiological status cells complex biological systems. As hardware artificial intelligence algorithms continue improve, expected become an increasingly powerful tool situ microelectromechanical devices combination with could development label-free, automatic, low-cost, fast recognition high-throughput responses different compounds, broad applications fields such as drug discovery, diagnostics, personalized medicine.

Language: Английский

Citations

34
Impedance‐Based Multimodal Electrical‐Mechanical Intrinsic Flow Cytometry DOI
Yongxiang Feng,

Junwen Zhu,

Huichao Chai

et al.

Small, Journal Year: 2023, Volume and Issue: 19(45)

Published: July 12, 2023

Abstract Reflecting various physiological states and phenotypes of single cells, intrinsic biophysical characteristics (e.g., mechanical electrical properties) are reliable important, label‐free biomarkers for characterizing cells. However, single‐modal or properties alone not specific enough to characterize cells accurately, it has been long challenging couple the conventionally image‐based characterization impedance‐based characterization. In this work, spatial‐temporal impedance sensing signal leveraged, an multimodal electrical‐mechanical flow cytometry framework on‐the‐fly high‐dimensional measurement is proposed, that is, Young's modulus E , fluidity β radius r cytoplasm conductivity σ i membrane capacitance C sm With characterization, can better reveal difference in cell types, demonstrated by experimental results with three types cancer (HepG2, MCF‐7, MDA‐MB‐468) 93.4% classification accuracy pharmacological perturbations cytoskeleton (fixed Cytochalasin B treated cells) 95.1% accuracy. It envisioned provides a new perspective accurate single‐cell

Language: Английский

Citations

32
Adaptive multiscale microscope with fast zooming, extended working distance, and large field of view DOI Creative Commons
Yi Zheng, Xin Wang,

Zhao Jiang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 1

Published: Jan. 1, 2024

The field-of-view (FOV), depth of field, and resolution conventional microscopes are constrained by each other; therefore, a zoom function is required. Traditional methods lose real-time performance have limited information throughput, severely limiting their application, especially in three-dimensional dynamic imaging large-amount or large-size sample scanning. Here, an adaptive multiscale (AMS) mechanism combining the benefits liquid lenses techniques proposed to realize functions fast zooming, wide working distance (WD) range large FOV on self-developed AMS microscope. design principles were revealed. Moreover, nonuniform-distortion-correction algorithm composite patching designed improve image quality. continuous tunable magnification microscope from 9× 18×, with corresponding diameters ranging 2.31 0.98 mm 161 287 line-pairs/mm, respectively. extended WD 0.8 response time 38 ms. Experiments demonstrated advantages pathological scanning, thick-sample imaging, microfluidic process monitoring, observation living microorganisms. first step towards technology expected be applied life sciences, medical diagnosis, industrial detection.

Language: Английский

Citations

14
Hydrodynamic shear stress’ impact on mammalian cell properties and its applications in 3D bioprinting DOI
Y. G. Zhang, Aidan P. O’Mahony, Yong He

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 022003 - 022003

Published: Jan. 26, 2024

As an effective cell assembly method, three-dimensional bioprinting has been widely used in building organ models and tissue repair over the past decade. However, different shear stresses induced throughout entire printing process can cause complex impacts on integrity, including reducing viability, provoking morphological changes altering cellular functionalities. The potential effects that may occur conditions under which these manifest are not clearly understood. Here, we review systematically how mammalian cells respond stress. We enumerate available experimental apparatus, categorise properties be affected disparate stress patterns. also summarise damaging mathematical as a predicting reference for design of systems. concluded it is essential to quantify specific resistance optimisation Besides, substantial positive impacts, inducing alignment promoting motility, generated by stress, suggest find proper range actively utilise its influences development future

Language: Английский

Citations

10
Viscoelasticity in 3D Cell Culture and Regenerative Medicine: Measurement Techniques and Biological Relevance DOI Creative Commons
Payam Eliahoo,

Hesam Setayesh,

Tyler Hoffman

et al.

ACS Materials Au, Journal Year: 2024, Volume and Issue: 4(4), P. 354 - 384

Published: June 18, 2024

The field of mechanobiology is gaining prominence due to recent findings that show cells sense and respond the mechanical properties their environment through a process called mechanotransduction. cells, cell organelles, extracellular matrix are understood be viscoelastic. Various technologies have been researched developed for measuring viscoelasticity biological materials, which may provide insight into both cellular mechanisms functions Here, we explain concept introduce major techniques used measure various soft materials in different length- timescale frames. topology material undergoing testing, geometry probe, magnitude exerted stress, resulting deformation should carefully considered choose proper technique each application. Lastly, discuss several applications 3D culture tissue models regenerative medicine, including organoids, organ-on-a-chip systems, engineered constructs, tunable viscoelastic hydrogels bioprinting cell-based therapies.

Language: Английский

Citations

9
Cytoskeleton-functionalized synthetic cells with life-like mechanical features and regulated membrane dynamicity DOI Creative Commons
Sebastian Novosedlik, Felix Reichel, Thijs W. van Veldhuisen

et al.

Nature Chemistry, Journal Year: 2025, Volume and Issue: 17(3), P. 356 - 364

Published: Jan. 3, 2025

The cytoskeleton is a crucial determinant of mammalian cell structure and function, providing mechanical resilience, supporting the membrane orchestrating essential processes such as division motility. Because its fundamental role in living cells, developing reconstituted or artificial major interest. Here we present an approach to construct that imparts support regulates dynamics. Our system involves amylose-based coacervates stabilized by terpolymer membrane, with formed from polydiacetylene fibrils. fibrils bundle due interactions positively charged amylose derivative, forming micrometre-sized structures mimicking cytoskeleton. Given intricate interplay between cellular design integration this represent advancement, paving way for development platforms exhibiting enhanced life-like behaviour.

Language: Английский

Citations

1