Neural Networks, Journal Year: 2025, Volume and Issue: unknown, P. 107209 - 107209
Published: Jan. 1, 2025
Language: Английский
Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2574 - 2594.e23
Published: May 1, 2024
High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.
Language: Английский
Citations
114
Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(5), P. 765 - 773
Published: April 24, 2023
Abstract Animals generate neural representations of their heading direction. Notably, in insects, direction is topographically represented by the activity neurons central complex. Although head cells have been found vertebrates, connectivity that endows them with properties unknown. Using volumetric lightsheet imaging, we find a topographical representation neuronal network zebrafish anterior hindbrain, where sinusoidal bump rotates following directional swims fish and otherwise stable over many seconds. Electron microscopy reconstructions show that, although cell bodies are located dorsal region, these arborize interpeduncular nucleus, reciprocal inhibitory stabilizes ring attractor encodes heading. These resemble those fly complex, showing similar circuit architecture principles may underlie across animal kingdom paving way to an unprecedented mechanistic understanding networks vertebrates.
Language: Английский
Citations
55
Science Advances, Journal Year: 2023, Volume and Issue: 9(8)
Published: Feb. 22, 2023
The advent of multimodal brain atlases promises to accelerate progress in neuroscience by allowing silico queries neuron morphology, connectivity, and gene expression. We used multiplexed fluorescent situ RNA hybridization chain reaction (HCR) technology generate expression maps across the larval zebrafish for a growing set marker genes. data were registered Max Planck Zebrafish Brain (mapzebrain) atlas, thus covisualization expression, single-neuron tracings, expertly curated anatomical segmentations. Using post hoc HCR labeling immediate early cfos, we mapped responses prey stimuli food ingestion freely swimming larvae. This unbiased approach revealed, addition previously described visual motor areas, cluster neurons secondary gustatory nucleus, which express calb2a, as well specific neuropeptide Y receptor, project hypothalamus. discovery exemplifies power this new atlas resource neurobiology.
Language: Английский
Citations
46
Cell, Journal Year: 2024, Volume and Issue: 187(3), P. 563 - 584
Published: Feb. 1, 2024
Biology spans a continuum of length and time scales. Individual experimental methods only glimpse discrete pieces this spectrum but can be combined to construct more holistic view. In Review, we detail the latest advancements in volume electron microscopy (vEM) cryo-electron tomography (cryo-ET), which together visualize biological complexity across scales from organization cells large tissues molecular details inside native cellular environments. addition, discuss emerging methodologies for integrating three-dimensional (3DEM) imaging with multimodal data, including fluorescence microscopy, mass spectrometry, single-particle analysis, AI-based structure prediction. This multifaceted approach fills gaps continuum, providing functional context, spatial organization, identity, interactions. We conclude perspective on incorporating diverse data into computational simulations that further bridge extend while dimension time.
Language: Английский
Citations
41
Exploration, Journal Year: 2024, Volume and Issue: 4(5)
Published: March 14, 2024
Abstract Neural interfaces, emerging at the intersection of neurotechnology and urban planning, promise to transform how we interact with our surroundings communicate. By recording decoding neural signals, these interfaces facilitate direct connections between brain external devices, enabling seamless information exchange shared experiences. Nevertheless, their development is challenged by complexities in materials science, electrochemistry, algorithmic design. Electrophysiological crosstalk mismatch electrode rigidity tissue flexibility further complicate signal fidelity biocompatibility. Recent closed‐loop brain‐computer while promising for mood regulation cognitive enhancement, are limited accuracy adaptability user interfaces. This perspective outlines challenges discusses progress contrasting non‐invasive invasive approaches, explores dynamics stimulation interfacing. Emphasis placed on applications beyond healthcare, highlighting need implantable high‐resolution capabilities.
Language: Английский
Citations
22
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(1)
Published: Jan. 3, 2025
Monitoring subcellular organelle dynamics in real time and precisely assessing membrane heterogeneity living cells are very important for studying fundamental biological mechanisms gaining a comprehensive understanding of cellular processes. However, there remains shortage effective tools these purposes. Herein, we propose strategy to develop the exchangeable water-sensing probeAPBD time-lapse imaging membrane-bound morphology with structured illumination microscopy at nanoscale. In this work, our results reveal mitochondria as first undergo morphological changes through swelling, fission, fusion cell necrosis, leading rupture endoplasmic reticulum (ER) sheet adhered mitochondria. Meanwhile, ER tubules then reconstructed by stretching autophagosomes. Moreover, APBD allows us directly visualize spatially resolved distribution biomembranes vs. water inside single mammalian cells. Our findings show that renal ischemia–reperfusion injury (IRI) model increased biomembrane cytoplasmic ratio tissue. This reveals intracellular between nucleus cytoplasm during IRI process. Overall, study presents development molecular dynamics.
Language: Английский
Citations
2
Current Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown
Published: June 13, 2020
Abstract High-resolution electron microscopy of nervous systems enables the reconstruction connectomes. A key piece missing information from connectomes is synaptic sign. We show that for D. melanogaster , artificial neural networks can predict transmitter type released at synapses micrographs and thus add putative signs to connections. Our network discriminates between six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) with an average accuracy 87%/94% synapses/entire neurons. developed explainability method reveal which features our using found significant ultrastructural differences classical transmitters. in two characterize morphological connection properties tens thousands neurons classed by predicted expression. find hemilineages largely express only one fastacting among their Furthermore, we different may differ like polarization projection targets.
Language: Английский
Citations
112
Nature, Journal Year: 2022, Volume and Issue: 608(7921), P. 146 - 152
Published: July 13, 2022
Abstract Social affiliation emerges from individual-level behavioural rules that are driven by conspecific signals 1–5 . Long-distance attraction and short-distance repulsion, for example, jointly set a preferred interanimal distance in swarms 6–8 However, little is known about their perceptual mechanisms executive neural circuits 3 Here we trace the neuronal response to self-like biological motion 9,10 , visual trigger developing zebrafish 2,11 Unbiased activity mapping targeted volumetric two-photon calcium imaging revealed 21 hotspots distributed throughout brain as well clustered biological-motion-tuned neurons multimodal, socially activated nucleus of dorsal thalamus. Individual thalamus encode local acceleration stimuli mimicking typical fish kinetics but insensitive global or continuous motion. Electron microscopic reconstruction synaptic input optic tectum projections into hypothalamic areas with conserved social function 12–14 Ablation selectively disrupted without affecting repulsion. This tectothalamic pathway thus serves recognition conspecifics, dissociates control repulsion during affiliation, revealing circuit underpinning collective behaviour.
Language: Английский
Citations
58
Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17
Published: April 17, 2023
One of the holy grails neuroscience is to record activity every neuron in brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently large-scale neural recording rodent models, single resolution across entire mammalian remains elusive. In contrast larval zebrafish offers great promise this regard. Zebrafish are a vertebrate model with substantial homology brain, but their transparency allows whole-brain recordings genetically-encoded fluorescent indicators at single-neuron using optical microscopy techniques. Furthermore begin show repertoire natural behavior from early age, including hunting small, fast-moving prey visual cues. Until work address bases these behaviors mostly relied on assays where fish was immobilized under microscope objective, stimuli such as were presented virtually. However significant progress has made developing imaging techniques for which not immobilized. Here we discuss recent advances, focusing particularly based light-field microscopy. We also draw attention several outstanding issues remain be addressed increase ecological validity results obtained.
Language: Английский
Citations
35