Exosome detection via surface-enhanced Raman spectroscopy for cancer diagnosis DOI Creative Commons
Juan Li, Yanru Li, Peilong Li

et al.

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 144, P. 1 - 14

Published: March 28, 2022

As nanoscale extracellular vesicles, exosomes are secreted by various cell types, and they widely distributed in multiple biological fluids. Studies have shown that tumor-derived can carry a variety of primary tumor-specific molecules, which may represent novel tool for the early detection cancer. However, clinical translation remains challenge due to requirement large quantities samples when enriching cancer-related fluids, insufficiency traditional techniques exosome subpopulations, complex isolation current commercially available phenotype profiling approaches. The evolving surface-enhanced Raman scattering (SERS) technology, with properties unique optoelectronics, easy functionalization, particular interaction between light metallic materials, achieve sensitive without multiplexed profiling, providing new mode real-time noninvasive analysis cancer patients. In present review, we mainly discussed based on SERS, especially SERS immunoassay. basic structure function were firstly introduced. Then, recent studies using technique critically reviewed, included substrates, modification SERS-based detection, combination other technologies diagnosis. This review systematically essential aspects, limitations, considerations applying technology cancer-derived exosomes, could provide valuable reference diagnosis through technology. Surface-enhanced has been applied obtain better diagnostic results. past three years, several reviews published narrowly focus methods detection. Selection surface functionalization substrate different will strategies exosomes. above aspects. emerging method is constantly contributing discovery diseases future.

Language: Английский

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics DOI Creative Commons

Simon F. Berlanda,

Maximilian Breitfeld,

Claudius Dietsche

et al.

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 93(1), P. 311 - 331

Published: Nov. 10, 2020

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRecent Advances in Microfluidic Technology for Bioanalysis and DiagnosticsSimon F. BerlandaSimon BerlandaDepartment of Biosystems Science Engineering, ETH Zurich, CH-8093 SwitzerlandMore by Simon BerlandaView Biography, Maximilian BreitfeldMaximilian BreitfeldDepartment BreitfeldView Claudius L. DietscheClaudius DietscheDepartment DietscheView Petra S. Dittrich*Petra DittrichDepartment Switzerland*Email: [email protected]More DittrichView Biographyhttp://orcid.org/0000-0001-5359-8403Cite this: Anal. Chem. 2021, 93, 1, 311–331Publication Date (Web):November 10, 2020Publication History Published online10 November 2020Published inissue 12 January 2021https://doi.org/10.1021/acs.analchem.0c04366Copyright © 2020 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views15908Altmetric-Citations94LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (3 MB) Get e-AlertsSUBJECTS:3D printing,Biotechnology,Fluid dynamics,Liquids,Microfluidic devices e-Alerts

Language: Английский

Citations

227
Characterizing Extracellular Vesicles and Their Diverse RNA Contents DOI Creative Commons
Eren M. Veziroglu, George I. Mias

Frontiers in Genetics, Journal Year: 2020, Volume and Issue: 11

Published: July 17, 2020

Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular communication agents that regulate physiological states by transporting biomolecules between near and distant cells. The research community has consistently advocated for the importance of RNA contents in demonstrating that: 1) EV-related can detected a liquid biopsy, 2) disease significantly alter contents, 3) sensitive specific biopsies implemented precision medicine settings measuring EV-derived contents. Furthermore, have medical potential beyond diagnostics. Both natural engineered being investigated therapeutic applications such as regenerative drug delivery agents. This review focuses specifically on EV characterization, analysis content, functional implications. NIH extracellular (ERC) program catapulted human from an profiling standpoint standardizing pipeline working with transcriptomics data, creating centralized database scientific community. There currently thousands RNA-sequencing profiles hosted Extracellular Atlas alone \citep{Murillo2019}, encompassing variety biofluid types health conditions. While number significant discoveries been made through these studies individually, integrative analyses data thus far limited. A primary focus ERC over next five years is bring higher resolution tools so investigators isolate analyze sub-populations, ultimately single sourced discrete cell types, tissues, complex biofluids. Higher techniques will essential evaluating roles circulating at level which impacts clinical decision making. We expect advances microfluidic technologies drive near-term innovation about diverse EVs. Long-term translation EV-based mainstay diagnostic tool depend upon identifying robust patterns genetic material correlate change status.

Language: Английский

Citations

196
Analyzing bacterial extracellular vesicles in human body fluids by orthogonal biophysical separation and biochemical characterization DOI
Joeri Tulkens, Olivier De Wever, An Hendrix

et al.

Nature Protocols, Journal Year: 2019, Volume and Issue: 15(1), P. 40 - 67

Published: Nov. 27, 2019

Language: Английский

Citations

182
Technology insight: Plant-derived vesicles—How far from the clinical biotherapeutics and therapeutic drug carriers? DOI

Minghui Cong,

Shenyu Tan,

Simin Li

et al.

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 182, P. 114108 - 114108

Published: Jan. 3, 2022

Language: Английский

Citations

164
Extracellular Vesicles as Signaling Mediators and Disease Biomarkers across Biological Barriers DOI Open Access
Pasquale Simeone,

Giuseppina Bologna,

Paola Lanuti

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(7), P. 2514 - 2514

Published: April 4, 2020

Extracellular vesicles act as shuttle vectors or signal transducers that can deliver specific biological information and have progressively emerged key regulators of organized communities cells within multicellular organisms in health disease. Here, we survey the evolutionary origin, general characteristics, significance extracellular mediators intercellular signaling, discuss various subtypes thus far described principal methodological approaches to their study, review role tumorigenesis, immunity, non-synaptic neural communication, vascular-neural communication through blood-brain barrier, renal pathophysiology, embryo-fetal/maternal placenta.

Language: Английский

Citations

160
Current Knowledge on Exosome Biogenesis, Cargo-Sorting Mechanism and Therapeutic Implications DOI Creative Commons
Shenmin Xie, Qin Zhang, Li Jiang

et al.

Membranes, Journal Year: 2022, Volume and Issue: 12(5), P. 498 - 498

Published: May 6, 2022

Extracellular vesicles (EVs) are nanoscale membrane released by donor cells that can be taken up recipient cells. The study of EVs has the potential to identify unknown cellular and molecular mechanisms in intercellular communication disease. Exosomes, with an average diameter ≈100 nanometers, a subset EVs. Different families have been shown involved formation exosomes subsequent secretion exosomes, which largely leads complexity form, structure function exosomes. In addition, because their low immunogenicity ability transfer variety bioactive components cells, regarded as effective drug delivery systems. This review summarizes known biogenesis, cargo loading, release bioengineering, is great importance for further exploration into clinical applications

Language: Английский

Citations

147
Isolation of extracellular vesicles with combined enrichment methods DOI Creative Commons
Janine Stam, Sabine Bartel, Rainer Bischoff

et al.

Journal of Chromatography B, Journal Year: 2021, Volume and Issue: 1169, P. 122604 - 122604

Published: Feb. 27, 2021

Extracellular vesicles (EVs) are currently of tremendous interest in many research disciplines and EVs have potential for development EV diagnostics or therapeutics. Most well-known single isolation methods their particular advantages disadvantages terms purity yield. Combining provides additional to improve the efficacy both This review assesses contribution using combined by performing a two-step systematic literature analysis from all papers applying year 2019. resulted an overview various being applied isolations. A second database was generated studies within first that fairly compared multiple determining yield after isolation. From these databases it is shown most used not per definition best based on yield, indicating more factors play role choice which method choose than only method. included ~60% isolations were performed with methods. The majority differential ultracentrifugation alone combination ultrafiltration. When determined clearly outperformed methods, regardless type starting material used. recommended point would be use size-exclusion chromatography since this method, especially when low-speed centrifugation, highest purity, while still providing reasonable

Language: Английский

Citations

145
Comprehensive isolation of extracellular vesicles and nanoparticles DOI Open Access
Qin Zhang, Dennis K. Jeppesen,

James N. Higginbotham

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(5), P. 1462 - 1487

Published: March 13, 2023

Language: Английский

Citations

134
Review of the current state of protein aggregation inhibition from a materials chemistry perspective: special focus on polymeric materials DOI Creative Commons
Robin Rajan, Sana Ahmed, Neha Sharma

et al.

Materials Advances, Journal Year: 2021, Volume and Issue: 2(4), P. 1139 - 1176

Published: Jan. 1, 2021

This review discusses various aspects of protein aggregation and inhibition strategies, emphasizing the use polymers, which is one most promising approaches to combat aggregation-induced complications in neurodegenerative diseases therapeutics.

Language: Английский

Citations

131
Extracellular Vesicles under Oxidative Stress Conditions: Biological Properties and Physiological Roles DOI Creative Commons
Elisabetta Chiaradia, Brunella Tancini, Carla Emiliani

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(7), P. 1763 - 1763

Published: July 12, 2021

Under physio-pathological conditions, cells release membrane-surrounded structures named Extracellular Vesicles (EVs), which convey their molecular cargo to neighboring or distant influencing metabolism. Besides involvement in the intercellular communication, EVs might represent a tool used by eliminate unnecessary/toxic material. Here, we revised literature exploring link between and redox biology. The first proof of this derives from evidence demonstrating that healthy protect target oxidative insults through transfer antioxidants. Oxidative stress conditions influence that, turn, modulate status cells. stress-related exert both beneficial harmful effects, as they can carry antioxidants ROS-generating enzymes oxidized molecules. As mediators cell-to-cell are also implicated pathophysiology diseases. review found numerous studies speculated on role signaling pathologies, but few them unraveled mechanisms behind complex link. Thus, purpose is report discuss evidence, highlighting analysis content stress-released (reminiscent originating cells), starting point for use diagnostic therapeutic tools

Language: Английский

Citations

129