RNAi‐mediated pest control targeting the Troponin I (wupA) gene in sweet potato weevil, Cylas formicarius DOI
Mengjun Zhang,

Xiaxuan Zhang,

Tingting Chen

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Abstract The sweet potato weevil ( Cylas formicarius ) is a critical pest producing enormous global losses in crops. Traditional management approaches for weevil, primarily using chemical pesticides, causes pollution, food safety issues, and harming natural enemies. While RNA interference (RNAi) promising environmentally friendly approach to control, its efficacy controlling the has not been extensively studied. In this study, we selected potential target C. , Troponin I gene wupA ), which essential musculature composition crucial fundamental life activities. We determined that abundantly expressed throughout all developmental stages of weevil. evaluated efficiency double‐stranded RNAs silencing via microinjection oral feeding larvae at different ages. Our findings demonstrate both significantly reduced expression produced high mortality. Moreover, 1st instar administered ds exhibited significant growth inhibition. assessed toxicity on no‐target insect silkworm safety. study indicates knockdown can inhibit development offer control.

Language: Английский

Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges DOI Creative Commons
Olivier Christiaens, Steve Whyard, Ana M. Vélez

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: April 21, 2020

Exploiting the RNA interference (RNAi) gene silencing mechanism to silence essential genes in pest insects, leading toxic effects, has surfaced as a promising new control strategy past decade. While first commercial RNAi-based products are currently coming market, application against wide range of insect species is still hindered by number challenges. In this review, we discuss current status these and different delivery strategies which insects can be targeted RNAi-triggering double-stranded (dsRNA) molecules. Furthermore, review also addresses physiological cellular barriers, lead decreased RNAi efficacy insects. Finally, novel non-transgenic technologies, such polymer or liposomic nanoparticles, peptide-based vehicles viral-like particles, discussed, could overcome barriers effective control.

Language: Английский

Citations

230
Strategies for mitigation of pesticides from the environment through alternative approaches: A review of recent developments and future prospects DOI
Veena Chaudhary, Mukesh Kumar, Chetan Chauhan

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 354, P. 120326 - 120326

Published: Feb. 21, 2024

Language: Английский

Citations

16
Integrating speed breeding with artificial intelligence for developing climate-smart crops DOI Open Access
Krishna Kumar

Molecular Biology Reports, Journal Year: 2022, Volume and Issue: 49(12), P. 11385 - 11402

Published: Aug. 8, 2022

Language: Английский

Citations

48
RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions DOI Creative Commons

Molly Darlington,

Jordan D. Reinders, Amit Sethi

et al.

Insects, Journal Year: 2022, Volume and Issue: 13(1), P. 57 - 57

Published: Jan. 5, 2022

The western corn rootworm (WCR), Diabrotica virgifera LeConte, is considered one of the most economically important pests maize (Zea mays L.) in United States (U.S.) Corn Belt with costs management and yield losses exceeding USD ~1–2 billion annually. WCR has proven challenging given ability this insect to evolve resistance multiple strategies including synthetic insecticides, cultural practices, plant-incorporated protectants, generating a constant need develop new tools. One recent developments expressing double-stranded hairpin RNA structures targeting housekeeping genes, which triggers an interference (RNAi) response eventually leads death. Following first description planta RNAi 2007, traits genes have been explored. In June 2017, U.S. Environmental Protection Agency approved product against insects for commercial use. This expresses dsRNA snf7 gene combination Bt proteins (Cry3Bb1 Cry34Ab1/Cry35Ab1) improve trait durability will be introduced use 2022.

Language: Английский

Citations

41
Targets selection and field evaluation of an RNA biopesticide to control Phyllotreta striolata DOI

Saibo Xu,

Xuezhi Zhang,

Yinuo Wang

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 209, P. 106330 - 106330

Published: Feb. 19, 2025

Language: Английский

Citations

1
Biotechnological Approaches for Host Plant Resistance to Insect Pests DOI Creative Commons

Pritam Kumari,

Poonam Jasrotia, Deepak Kumar

et al.

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: June 2, 2022

Annually, the cost of insect pest control in agriculture crosses billions dollars around world. Until recently, broad-spectrum synthetic pesticides were considered as most effective means agriculture. However, over years, overreliance on has caused adverse effects beneficial insects, human health and environment, led to development pesticide resistant insects. There is a critical need for alternative management strategies aiming minimum use conservation natural enemies maintaining ecological balance environment. Host plant resistance plays vital role integrated but insect-resistant varieties through conventional ways host takes time, challenging it involves many quantitative traits positioned at various loci. Biotechnological approaches such gene editing, transformation, marker-assisted selection etc. this direction have recently opened up new era options. These could contribute towards about exploring much wider array novel insecticidal genes that would otherwise be beyond scope breeding. interventions can alter expression level pattern well transgenic with improve by providing access molecules. This review will discuss emerging biotechnological tools available develop engineered crop genotypes better ability resist attack pests.

Language: Английский

Citations

36
Application progress of plant-mediated RNAi in pest control DOI Creative Commons
Xiang Li, Xiaoguang Liu,

Wenhui Lu

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Aug. 8, 2022

RNA interference (RNAi)-based biopesticides are novel biologic products, developed using RNAi principles. They engineered to target genes of agricultural diseases, insects, and weeds, interfering with their gene expression so as hinder growth alleviate damaging effects on crops. RNAi-based broadly classified into resistant plant-based plant-incorporated protectants (PIPs) non-plant-incorporated protectants. PIP that combine the advantages transgenic Such through nuclear or plastid transformation breed plants, i.e., dsRNA-expressing plants. The dsRNA is expressed in plant cell, pest disease control being achieved plant-target organism interactions. Here, we review action mechanism strategies for management, development plant, current status deploying these products control, well future research directions problems production commercialization. Overall, this study aims elucidate provide guidelines research.

Language: Английский

Citations

28
A Review of Alternative Management Tactics Employed for the Control of Various Cockroach Species (Order: Blattodea) in the USA DOI Creative Commons
Ameya D. Gondhalekar, Arthur G. Appel,

Gretchen M. Thomas

et al.

Insects, Journal Year: 2021, Volume and Issue: 12(6), P. 550 - 550

Published: June 12, 2021

Effective control of domestic and peridomestic cockroaches requires integrated approaches that emphasize concurrent use chemicals with alternative tactics. An pest management (IPM) approach is particularly justified in environments where satisfactory cockroach cannot be achieved due to multiple factors including development insecticide aversion resistance some species, poor sanitation or structural issues foster infestations. While a flurry research effort has been devoted study tactics for control, only few them have evaluated the context IPM programs. This review focuses on examining studies are proven efficacious, economical, logistically feasible their inclusion programs important USA. Management educate public biology, behavior, importance sanitation; traps monitor infestation levels; apply targeted low impact insecticides such as baits, demonstrated greater success effective sustainable when compared an insecticide-only approach. Incorporation other methods will require more applied validates real-world scenarios demonstrates cost-effectiveness.

Language: Английский

Citations

31
Highly Variable Dietary RNAi Sensitivity Among Coleoptera DOI Creative Commons
Jonathan Willow, Eve Veromann

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 7, 2021

Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and forest trees, plant biotechnology solutions are being developed with the hope of limiting these losses. Due unprecedented target-specificity double-stranded RNA (dsRNA), its utility inducing interference (RNAi) when consumed by target pest species, dsRNA-based approaches represent cutting edge current pesticide research development. We review dietary RNAi studies coleopterans discuss prospects future directions regarding RNAi-based management coleopteran pests. Herein, we also provide a balanced overview existing order an accurate re-assessment sensitivity coleopterans, despite limitations body scientific literature. further impediments our understanding this important insect identify critical for area, emphasis on using approaches.

Language: Английский

Citations

28
Plant Recombinant Gene Technology for Pest Control in the Twenty-First Century: From Simple Transgenesis to CRISPR/Cas DOI
Ivan M. Dubovskiy, Ekaterina V. Grizanova, Sophia V. Gerasimova

et al.

Published: Jan. 1, 2024

The current progress in genetic technologies (methodologies) allows to create crops with the modified genome counter different pest species. Genome editing is most popular and efficient approach increase crop defense against harmful insects, mites, nematodes. During first decades of twenty-first century, methodology control using plant recombinant has evolved significantly. Toxins bacteria Bacillus thuringiensis (Bt) were heterologous protective compounds introduced into plants, until now, these remain main source transgenes for control. Heterologous expression proteins toxic pests, such as lectins protease inhibitors, was another strategy increasing resistance plants. RNA interference genes achieved through plants recently become studied area protection. CRISPR/Cas-based modification systems modern gene manipulation allow knock out genes, targeted variation, include precise insertion/deletion single nucleotide changes. All approaches are focused on targeting some physiological, biochemical, behavioral pests. This chapter summarizes improvement protection We extensively reviewed sum research articles published between 2000 2022 reporting that successful transgenic events proved be a wide range

Language: Английский

Citations

4