Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA

RNA Biology, Journal Year: 2021, Volume and Issue: 18(11), P. 1747 - 1759

Published: Jan. 4, 2021

RNAi is a potent technique for the knockdown of target genes. However, its potential off-target effects limit widespread applications in both reverse genetic analysis and manipulation. Previous efforts have uncovered rules underlying specificity siRNA-based silencing, which has broad humans, but basis dsRNAs, are better suited use as insecticides, poorly understood. Here, we investigated governing dsRNA specificity. Mutational analyses showed that dsRNAs with >80% sequence identity genes triggered efficiently. ≥16 bp segments perfectly matched or >26 almost one two mismatches scarcely distributed (single inserted between ≥5 matching mismatched couplets ≥8 segments) also able to trigger RNAi. Using these parameters predict risk, can be designed optimize efficiency, paving way widespread, rational application pest control.

Language: Английский

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DsRNA-based pesticides: Considerations for efficiency and risk assessment

Chemosphere, Journal Year: 2024, Volume and Issue: 352, P. 141530 - 141530

Published: Feb. 22, 2024

In view of the ongoing climate change and ever-growing world population, novel agricultural solutions are required to ensure sustainable food supply. Microbials, natural substances, semiochemicals double stranded RNAs (dsRNAs) all considered potential low risk pesticides. DsRNAs function at molecular level, targeting specific regions genes organisms, provided that they share a minimal sequence complementarity approximately 20 nucleotides. Thus, dsRNAs may offer great alternative conventional chemicals in environmentally friendly pest control strategies. Any low-risk pesticide needs be efficient exhibit toxicological environmental persistence. Having said that, current review, mode dsRNA action is explored parameters need taken into consideration for development dsRNA-based pesticides highlighted. Moreover, since differs from those synthetic pesticides, custom-made assessment schemes thus, critical issues related discussed here.

Language: Английский

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Crop protection by RNA interference: a review of recent approaches, current state of developments and use as of 2013

Environmental Sciences Europe, Journal Year: 2025, Volume and Issue: 37(1)

Published: Jan. 25, 2025

Language: Английский

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Insect pest management in the age of synthetic biology

Plant Biotechnology Journal, Journal Year: 2021, Volume and Issue: 20(1), P. 25 - 36

Published: Aug. 20, 2021

Arthropod crop pests are responsible for 20% of global annual losses, a figure predicted to increase in changing climate where the ranges numerous species projected expand. At same time, many insect beneficial, acting as pollinators and predators pest species. For thousands years, humans have used increasingly sophisticated chemical formulations control but, scale agriculture expanded meet needs population, concerns about negative impacts agricultural practices on biodiversity grown. While biological solutions, such agents pheromones, previously had relatively minor roles management, biotechnology has opened door new approaches controlling pests. In this review, we look at how advances synthetic biology providing options control. We discuss emerging technologies engineering resistant crops populations examine biomanufacturing that enabling production products

Language: Английский

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Going Viral: Virus-Based Biological Control Agents for Plant Protection

Annual Review of Phytopathology, Journal Year: 2022, Volume and Issue: 60(1), P. 21 - 42

Published: March 18, 2022

The most economically important biotic stresses in crop production are caused by fungi, oomycetes, insects, viruses, and bacteria. Often chemical control is still the commonly used method to manage them. However, development of resistance different pathogens/pests, putative damage on natural ecosystem, toxic residues field, and, thus, contamination environment have stimulated search for saferalternatives such as use biological agents (BCAs). Among BCAs, a major driver controlling host populations evolution, somewhat underused, mostly because regulatory hurdles that make cost registration host-specific BCAs not affordable comparison with limited potential market. Here, we provide comprehensive overview state art virus-based against bacteria, specific focus new approaches rely only direct biocidal virus component but also complex ecological interactions between viruses their hosts do necessarily result host.

Language: Английский

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RNAi Crop Protection Advances

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(22), P. 12148 - 12148

Published: Nov. 10, 2021

RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There plenty of evidence its use through host-induced gene silencing (HIGS) emerging that spray-induced (SIGS) techniques can work as well to control viruses, bacteria, fungi, insects, nematodes. For SIGS, most significant challenge achieving stability avoiding premature degradation in the environment or during absorption by target organism. One encapsulation liposomes, virus-like particles, polyplex nanoparticles, bioclay, which be obtained recombinant production vectors, transgenesis, micro/nanoencapsulation. The materials must biodegradable, stable multiple chemical environments, favoring controlled release RNAi. Most current research on encapsulated focuses primarily oral delivery insects essential genes. regulation risk assessment using different approaches; however, this has positive economic, environmental, human health implications agriculture. emergence alternatives combining with induction resistance crops elicitation metabolic expected, biotechnological optimization large-scale production.

Language: Английский

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RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges

Plants, Journal Year: 2021, Volume and Issue: 10(4), P. 650 - 650

Published: March 29, 2021

Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent significant threat to agriculture worldwide. Conventional approaches based use pesticides raise social concern for impact environment human health alternative control methods urgently needed. The rapid improvement extensive implementation RNA interference (RNAi) technology various model non-model organisms has provided initial framework adapt this post-transcriptional gene silencing management fungal pathogens. Recent studies showed that exogenous application double-stranded (dsRNA) molecules targeting growth virulence-related genes disease attenuation pathogens like Botrytis cinerea, Sclerotinia sclerotiorum Fusarium graminearum in different hosts. Such results highlight RNAi holds great potential RNAi-mediated plant control. Production dsRNA can be possible by using either in-vitro or in-vivo synthesis. In review, we describe involved discuss production, formulation, delivery methods. Potential challenges faced while developing strategy pathogens, such as off-target epigenetic effects, with their solutions also discussed.

Language: Английский

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Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Plants, Journal Year: 2022, Volume and Issue: 11(14), P. 1856 - 1856

Published: July 15, 2022

Anthracnose is a severe disease caused by Colletotrichum spp. on several crop species. Fungal infections can occur both in the field and at post-harvest stage causing lesions fruits economic losses. Physical treatments synthetic fungicides have traditionally been preferred means to control anthracnose adverse effects; however, urgent need decrease use of toxic chemicals led investigation innovative sustainable protection techniques. Evidence for efficacy biological agents vegetal derivates has reported; their introduction into actual strategies requires solutions critical issues. Biotechnology-based approaches also explored, revealing opportunity develop safe methods management through genome editing RNA interference technologies. Nevertheless, besides number advantages related use, e.g., putative absence effects due high specificity, aspects remain be clarified enable Integrated Pest Management (IPM) protocols against disease.

Language: Английский

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Current and future prospects of “all-organic” nanoinsecticides for agricultural insect pest management

Frontiers in Nanotechnology, Journal Year: 2023, Volume and Issue: 4

Published: Jan. 9, 2023

Graphical Abstract

Language: Английский

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The N‐terminal subunit of vitellogenin in planthopper eggs and saliva acts as a reliable elicitor that induces defenses in rice

New Phytologist, Journal Year: 2023, Volume and Issue: 238(3), P. 1230 - 1244

Published: Feb. 6, 2023

Summary Vitellogenins (Vgs) are critical for the development and fecundity of insects. As such, these essential proteins can be used by plants to reliably sense presence We addressed this with a combination molecular chemical analyses, genetic transformation, bioactivity tests, insect performance assays. The small N‐terminal subunit Vgs planthopper Nilaparvata lugens (NlVgN) was found trigger strong defense responses in rice when it enters during feeding or oviposition insect. defenses induced NlVgN not only decreased hatching rate N. eggs, but also volatile emissions plants, which rendered them attractive common egg parasitoid. VgN other planthoppers triggered same rice. further show that deposited compared induces somewhat different response, probably target appropriate developmental stage confirm is growth, development, fecundity. This study demonstrates eggs saliva acts as reliable unavoidable elicitor plant defenses. Its importance precludes evolutionary adaptions prevent detection plants.

Language: Английский

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