A novel pH‐responsive nanoniosomal emulsion for sustained release of curcumin from a chitosan‐based nanocarrier: Emphasis on the concurrent improvement of loading, sustained release, and apoptosis induction

Biotechnology Progress, Journal Year: 2022, Volume and Issue: 38(5)

Published: June 9, 2022

Curcumin application as an anti-cancer drug is faced with several impediments. This study has developed a platform that facilitates the sustained release of curcumin, improves loading efficiency, and activity. Montmorillonite (MMT) nanoparticles were added to chitosan (CS)-agarose (Aga) hydrogel then loaded curcumin (Cur) prepare curcumin-loaded nanocomposite hydrogel. The capacity increased from 63% 76% by adding MMT chitosan-agarose Loading fabricated in nanoniosomal emulsion resulted under acidic conditions. Release kinetics analysis showed diffusion erosion are dominant mechanisms, indicating non-fickian (or anomalous) transport based on Korsmeyer-Peppas model. FTIR spectra confirmed all components present nanocomposite. Besides, XRD results corroborated amorphous structure prepared Zeta potential stability nanocarrier. Cytotoxicity CS-Aga-MMT-Cur MCF-7 cells was comparable curcumin-treated (p < 0.001). Moreover, percentage apoptotic due enhanced profile resulting addition incorporation into emulsion. To recapitulate, current delivery improved loading, release, effect. Hence, this could be candidate mitigate cancer therapy restrictions curcumin.

Language: Английский

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Shikonin inhibited glycolysis and sensitized cisplatin treatment in non-small cell lung cancer cells via the exosomal pyruvate kinase M2 pathway

Bioengineered, Journal Year: 2022, Volume and Issue: 13(5), P. 13906 - 13918

Published: May 2, 2022

The active ingredient of the traditional Chinese medicine comfrey is shikonin, a naphthoquinone compound. focus this study was to investigate effect shikonin on proliferation, invasion, migration, and chemoresistance non-small cell lung cancer (NSCLC) cells, explore its underlying molecular biological mechanisms. results show that inhibited viability, migration NSCLC cells A549 PC9, induced apoptosis. As inhibitor pyruvate kinase M2 (PKM2), key enzyme in glycolysis, glucose uptake production lactate, final metabolite aerobic glycolysis. In vivo chemotherapeutic assay showed reduced tumor volume weight mice model increased sensitivity cisplatin chemotherapy. Histoimmunology experiments combination downregulated expression PKM2 transcriptionally regulated downstream gene transporter 1 (Glut1) tissue. an assessment metabolism, micro-PET/CT data fluorodeoxy (18F-FDG) into tumor. Since exosomal affected we also demonstrated could inhibit exosome secretion through administration GW4869. Furthermore, sensitized treatment by reducing extracellular PKM2. conclusion, suggest not only inhibits intracellularly but reduces glycolytic flux increases pathway.

Language: Английский

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Insight into the Biological Roles and Mechanisms of Phytochemicals in Different Types of Cancer: Targeting Cancer Therapeutics

Nutrients, Journal Year: 2023, Volume and Issue: 15(7), P. 1704 - 1704

Published: March 31, 2023

Cancer is a hard-to-treat disease with high reoccurrence rate that affects health and lives globally. The condition has occurrence the second leading cause of mortality after cardiovascular disorders. Increased research more profound knowledge mechanisms contributing to disease’s onset progression have led drug discovery development. Various drugs are on market against cancer; however, face challenges chemoresistance. other major problem side effects these drugs. Therefore, using complementary additional medicines from natural sources best strategy overcome issues. naturally occurring phytochemicals vast source novel various ailments. modes action by which show their anti-cancer can be induction apoptosis, cell cycle arrest, kinase inhibition, blocking carcinogens. This review aims describe different phytochemicals, classification, role as agents, mode in types cancer.

Language: Английский

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Transcriptional regulation and post-translational modifications in the glycolytic pathway for targeted cancer therapy

Acta Pharmacologica Sinica, Journal Year: 2024, Volume and Issue: 45(8), P. 1533 - 1555

Published: April 15, 2024

Language: Английский

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Reprogramming of glucose metabolism: The hallmark of malignant transformation and target for advanced diagnostics and treatments

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117257 - 117257

Published: Aug. 12, 2024

Reprogramming of cancer metabolism has become increasingly concerned over the last decade, particularly reprogramming glucose metabolism, also known as "Warburg effect". The is considered a novel hallmark human cancers. A growing number studies have shown that can regulate many biological processes cancers, including carcinogenesis, progression, metastasis, and drug resistance. In this review, we summarize major functions, clinical significance, potential targets signaling pathways metabolic in Moreover, applications natural products small molecule inhibitors targeting are analyzed, some agents trial statuses summarized, well pros cons for therapy analyzed. Overall, plays an important role prediction, prevention, diagnosis treatment Glucose reprogramming-related great to serve biomarkers improving individual outcomes prognosis patients. innovations related will be hotspot research future. We suggest more high-quality trials with abundant formulations toxicology experiments would beneficial development application drugs metabolism.This review provide researchers broader perspective comprehensive understanding about significance

Language: Английский

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Tyrosine Kinase Signaling in Cancer Metabolism: PKM2 Paradox in the Warburg Effect

Frontiers in Cell and Developmental Biology, Journal Year: 2018, Volume and Issue: 6

Published: July 24, 2018

The Warburg Effect, or aerobic glycolysis, is one of the major metabolic alterations observed in cancer. Hypothesized to increase a cell's proliferative capacity via regenerating NAD+, increasing pool glycolytic biosynthetic intermediates, and lactate production that affects tumor microenvironment, Effect important for growth proliferation cells. mechanisms by which cell acquires phenotype are regulated expression numerous oncogenes, including oncogenic tyrosine kinases. Oncogenic kinases play significant role phosphorylating regulating activity enzymes. Tyrosine phosphorylation enzymes increases activities majority enzymes, thus promoting increased rate proliferation. Paradoxically however, pyruvate kinase M2 isoform (PKM2) results decreased PKM2 this decrease promotes effect. Furthermore, recent studies have shown also able act as protein using phosphoenolpyruvate (PEP) substrate promote tumorigenesis. Therefore, investigated both classical non-canonical growth, raise further interesting questions. In review, we will summarize these advances revealing importance regulation well promotion growth.

Language: Английский

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Anti-Angiogenic Effects of Phytochemicals on miRNA Regulating Breast Cancer Progression

Biomolecules, Journal Year: 2020, Volume and Issue: 10(2), P. 191 - 191

Published: Jan. 27, 2020

Several phytochemicals have been identified for their role in modifying miRNA regulating tumor progression. miRNAs modulate the expression of several oncogenes and suppressor genes including that regulate angiogenesis. Hypoxia inducible factor-1 alpha (HIF-1α) signaling is a central axis activates oncogenic acts as metabolic switch endothelial cell (EC) driven Tumor angiogenesis by reprogramming EC crucial progression metastasis many different cancers, breast has linked to aberrant profiles. In current article, we identify context ECs review how selected could levels induce an anti-angiogenic action cancer. Studies involving genistein, epigallocatechin gallate (EGCG) resveratrol demonstrate regulation miRNA-21, miRNA-221/222 miRNA-27, which are prognostic markers triple negative cancers (TNBCs). Modulating pathway novel strategy controlling growth. Cardamonin, curcumin exhibit property targeting metabolism. Here suggest using target miRNAs, turn suppresses angiogenesis, should potential inhibit growth, progression, invasion may be developed into effective therapeutic treatment where plays significant growth

Language: Английский

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Curcumin-Induced DNA Demethylation in Human Gastric Cancer Cells Is Mediated by the DNA-Damage Response Pathway

Oxidative Medicine and Cellular Longevity, Journal Year: 2020, Volume and Issue: 2020, P. 1 - 13

Published: June 17, 2020

Curcumin, a natural polyphenol antioxidant extracted from the root of turmeric ( Curcuma longa ), can induce apoptosis and DNA demethylation in several types cancer cells. However, mechanism its anticancer potentials effects potential relationships between these outcomes have not been clearly elucidated. In present study, curcumin on proliferation, colony formation, migration human gastric cells (hGCCs) were explored. Reactive oxygen species (ROS) levels, mitochondrial damage, curcumin-treated hGCCs analyzed. Changes expression genes related to damage repair, p53 pathway, cell cycle, methylation following treatment also evaluated. We observed that inhibited dose- time-dependent fashion. A high concentration elevated ROS levels triggered hGCCs. Further, curcumin-induced was mediated by damaged repair-p53-p21/GADD45A-cyclin/CDK-Rb/E2F-DNMT1 axis. propose effect could largely be attributed prooxidative at concentrations elevation Moreover, we novel which induces hGCCs, suggesting need further investigate mechanisms other hypomethylating drugs.

Language: Английский

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Curcumin circumvent lactate-induced chemoresistance in hepatic cancer cells through modulation of hydroxycarboxylic acid receptor-1

The International Journal of Biochemistry & Cell Biology, Journal Year: 2020, Volume and Issue: 123, P. 105752 - 105752

Published: April 21, 2020

Language: Английский

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Curcumin’s Beneficial Effects on Neuroblastoma: Mechanisms, Challenges, and Potential Solutions

Biomolecules, Journal Year: 2020, Volume and Issue: 10(11), P. 1469 - 1469

Published: Oct. 22, 2020

Curcumin, a natural polyphenolic compound derived from the South Asian turmeric plant (Curcuma longa), has well-characterized antioxidant, anti-inflammatory, anti-protein-aggregate, and anticancer properties. Neuroblastoma (NB) is cancer of nervous system that arises primarily in pediatric patients. In order to reduce multiple disadvantages side effects conventional oncologic modalities potentially overcome drug resistance, substances such as curcumin are examined complementary supportive therapies against NB. NB cell lines, by itself promotes apoptosis cycle arrest through suppression serine–threonine kinase Akt nuclear factor kappa activated B-cells (NF-κB) signaling, induction mitochondrial dysfunction, upregulation p53 caspase signaling. While demonstrates anti-NB efficacy vitro, cross-validation between types currently lacking for many its specific mechanistic activities. Furthermore, curcumin’s low bioavailability oral administration, poor absorption, relative insolubility water pose challenges clinical introduction. Numerous formulations, including nanoparticles, nanocarriers, microemulsions, have been developed, with these having some success treatment future, standardization further basic preclinical trials will be required ensure safety formulations. administration clinically safe even at high doses, necessary substantiate practical prevention

Language: Английский

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