Advances and Perspectives in the Use of Biocontrol Agents against Fungal Plant Diseases

Horticulturae, Journal Year: 2022, Volume and Issue: 8(7), P. 577 - 577

Published: June 25, 2022

The use of synthetic fungicides to control fungal diseases has growing limitations due eco-toxicological risks. Therefore, it is necessary replace or integrate high risk chemicals with safer tools for human health and environment. Consequently, research on the selection, evaluation, characterization, biocontrol agents (BCAs) consistently increased in last decades. BCA formulates, particularly some countries, are still scarce coping demand their sustainable agricultural management. To foster development utilization new effective bioformulates, there a need optimize activity, share knowledge formulation processes simplify registration procedures. Studies based molecular can significantly contribute achieve such objectives. present review provides state art plant special emphasis (i) features most studied BCAs; (ii) key strategies selection BCAs (iii); mechanisms action main (iv) metagenomic studies (v) issues constraints commercialization BCAs, (vi) perspectives diseases.

Language: Английский

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Biology and applications of Clonostachys rosea

Journal of Applied Microbiology, Journal Year: 2020, Volume and Issue: 129(3), P. 486 - 495

Published: March 2, 2020

Clonostachys rosea is a promising saprophytic filamentous fungus that belongs to phylum Ascomycota. widespread around the world and exists in many kinds of habitats, with highest frequency soil. As an excellent mycoparasite, C. exhibits strong biological control ability against numerous fungal plant pathogens, nematodes insects. These behaviours are based on activation multiple mechanisms such as secreted cell-wall-degrading enzymes, production antifungal secondary metabolites induction defence systems. Besides having significant biocontrol activity, also functions biodegradation plastic waste, biotransformation bioactive compounds, bioenergy sources fermentation. This mini review summarizes information about biology various applications expands its possible uses.

Language: Английский

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LysM Proteins Regulate Fungal Development and Contribute to Hyphal Protection and Biocontrol Traits in Clonostachys rosea

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: April 16, 2020

Lysin motif (LysM) modules are approximately 50 amino acids long and bind to peptidoglycan, chitin its derivatives. Certain LysM proteins in plant pathogenic entomopathogenic fungi shown scavenge oligosaccharides thereby dampen host defence reactions. Other can protect the fungal cell wall against hydrolytic enzymes. In this study, we investigated biological function of mycoparasitic fungus Clonostachys rosea. The C. rosea genome contained three genes coding for LysM-containing gene expression analysis revealed that lysm1 lysm2 were induced during interaction with Fusarium graminearum colonization wheat roots. Lysm1 was suppressed germinating conidia, while growth or peptidoglycan-containing medium. Deletion resulted mutants increased levels conidiation conidial germination, but reduced ability control diseases caused by F. Botrytis cinerea. Δlysm2 strain showed a distinct, accelerated mycelial disintegration phenotype accompanied biomass production, suggesting role LYSM2 hyphal protection endogenously produced degrading Δlysm1Δlysm2 strains displayed colonize roots, only failed supress response PR1 PR4. Based on our data, propose LYSM1 as regulator development endogenous enzymes, both required responses. Our findings expand understanding fungal-fungal interactions biocontrol.

Language: Английский

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An anecdote of mechanics for Fusarium biocontrol by plant growth promoting microbes

Biological Control, Journal Year: 2022, Volume and Issue: 174, P. 105012 - 105012

Published: Aug. 6, 2022

Language: Английский

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Small RNAs: A new paradigm in fungal-fungal interactions used for biocontrol

Fungal Biology Reviews, Journal Year: 2024, Volume and Issue: 48, P. 100356 - 100356

Published: Feb. 13, 2024

In many eukaryotes, small RNAs (sRNAs) can mediate gene expression regulation through a mechanism known as RNA silencing. fungi, silencing plays crucial role in numerous biological processes, including parasitic and mutualistic fungus-plant interactions. This review summarizes recent findings on the of fungus-fungus fungus-insect interactions relation to their use for control (biocontrol) fungal plant diseases insect damage. Genes belonging machinery are identified genomes almost all oomycete biocontrol organisms. However, functional genetic studies Ascomycota species Hypocreales order, such Trichoderma atroviride Clonostachys rosea, show how have family-specific effects, conidiation is affected differently two organisms when same elements deleted. The size sRNAs regulated by also vary between Cross-species represents new field study antagonistic For example, microRNA (miRNA) another hypocrealean fungus, Beauveria bassiana, was proven target genes involved immune response mosquitoes, there indications that miRNAs from mycoparasitic C. rosea may factors virulence its plant-pathogenic host fungi. Accumulating evidence shows number endogenous disruption always much higher than predicted direct genes. As several putative targets transcription factors, it possible specific master regulators expression, affecting high cascading regulating effects. challenges faced studying cross-species silencing, sRNA trafficking during mycoparasitism, discussed. includes difficulties separating extracellular vesicles fungi those hosts, amount sequencing reads lost bioinformatics filtering steps, imprecise prediction lack streamlined accepted way reporting results.

Language: Английский

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Biocontrol and Plant-Growth-Promoting Traits of Talaromyces apiculatus and Clonostachys rosea Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm

Microorganisms, Journal Year: 2020, Volume and Issue: 8(8), P. 1138 - 1138

Published: July 28, 2020

Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important in oil palms South East Asia. Unfortunately, there no single effective control measure available. Tremendous efforts have been directed incorporation of environmentally friendly biocontrol approaches minimizing BSR disease. This study investigated performance two potential agents (BCAs), AAT0115 and AAB0114 strains recovered from palm on suppression planta, also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated ascomycetous Talaromyces apiculatus (Ta) AT0115 Clonostachys rosea (Cr) species with PGP characteristics. In vitro studies demonstrated both Ta Cr are capable reducing linear mycelial growth G. boninense. Inoculation individual Ta-as well as Cr+Ta consortium-induced a significant increment leaf area bole girth oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At post-inoculation, shoot root biomass, nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium boron) were significantly higher Ta-inoculated compared to treated non-Ta-inoculated maize. Chlorophyll carotenoids rapidly growing challenged Cr, or combination not negatively affected. consortium had 4.9-60% reduction untreated control. Co-inoculation resulted increased efficiencies 18-26% (compared only) 48-55% only). Collectively, Ta, either individually showed while possess traits palm.

Language: Английский

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Insights into the ecological generalist lifestyle of Clonostachys fungi through analysis of their predicted secretomes

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 16, 2023

The fungal secretome comprise diverse proteins that are involved in various aspects of lifestyles, including adaptation to ecological niches and environmental interactions. aim this study was investigate the composition activity secretomes mycoparasitic beneficial fungal-plant interactions.We used six Clonostachys spp. exhibit saprotrophic, mycotrophic plant endophytic lifestyles. Genome-wide analyses performed composition, diversity, evolution gene expression relation their potential role lifestyles.Our showed predicted analyzed species comprised between 7 8% respective proteomes. Mining transcriptome data collected during previous studies 18% genes encoding secreted were upregulated interactions with mycohosts Fusarium graminearum Helminthosporium solani. Functional annotation revealed most represented protease family subclass S8A (11-14% total), which include members shown be response nematodes mycohosts. Conversely, numerous lipases carbohydrate-active enzyme (CAZyme) groups appeared potentially eliciting defense responses plants. For example, analysis identified nine CAZyme orthogroups evolving for gains (p ≤ 0.05), hemicellulose degradation, producing defense-inducing oligomers. Moreover, 8-10% composed cysteine-enriched proteins, hydrophobins, important root colonization. Effectors more numerous, comprising 35-37% secretomes, where certain belonged seven induced C. rosea F. or H. Furthermore, considered possessed high numbers containing Common Fungal Extracellular Membranes (CFEM) modules, known virulence. Overall, improves our understanding establishes a basis future investigation aiming at sustainable biocontrol diseases.

Language: Английский

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Clonostachys Genus as a Source of Metabolites for Agricultural Applications

Fungal biology, Journal Year: 2025, Volume and Issue: unknown, P. 233 - 248

Published: Jan. 1, 2025

Language: Английский

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Exploring the synergistic potential of Trichoderma gamsii T6085 and Clonostachys rosea IK726 for biological control of Fusarium head blight in wheat

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128152 - 128152

Published: March 1, 2025

Language: Английский

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Colletotrichum falcatum Proteome Unraveled the Promising Functions of Myosin-1 and Polyketide Synthase Proteins in Instigating the Pathogen Virulence

Deleted Journal, Journal Year: 2025, Volume and Issue: 77(3)

Published: April 17, 2025

Language: Английский

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