Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2781 - 2781

Published: April 16, 2020

Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm in different ruminant species. However, significant amount still suffers considerable cryodamage, which may affect quality and fertility. Recently, use "omics" technologies cryobiology, especially proteomics studies, has led better understanding molecular modifications induced by cryopreservation, facilitating identification freezability biomarkers certain proteins that can be added before enhance cryosurvival. This review provides an updated overview mechanisms involved are part responsible structural, functional fertility changes observed frozen-thawed sperm. Moreover, basis those factors freezing resilience species is also discussed as well aspects novel strategies developed reduce including new cryoprotectants, antioxidants, proteins, nanoparticles vitrification.

Language: Английский

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Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Reproduction and Fertility, Journal Year: 2022, Volume and Issue: 3(3), P. R121 - R146

Published: July 1, 2022

Biodiversity is defined as the presence of a variety living organisms on Earth that essential for human survival. However, anthropogenic activities are causing sixth mass extinction, threatening even our own species. For many animals, dwindling numbers becoming fragmented populations with low genetic diversity, long-term species viability. With extinction rates 1000-10,000 times greater than natural,

Language: Английский

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Cryopreservation of Small Ruminant Sperm and Detection of Sperm Cryocapacitation

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 165 - 182

Published: Jan. 1, 2025

Language: Английский

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Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(4), P. 560 - 563

Published: Jan. 16, 2023

Eighty-five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC-PSA/PI chlortetracycline used for imaging flow cytometry evaluation of viability, mitochondrial membrane potential (MMP), acrosome integrity capacitation, respectively. Sperm motility was also registered. (46.1 ± 7.7 vs. 24.1% 6.5%), viability (49.8 11.5 32.3% 9.6%) high MMP (49.8% 12.4% 34.9% 9.9%) decreased significantly (p < .05) during cryopreservation process, in contrast to acrosome-reacted viable spermatozoa (1.0% 1.6% 1.0% 1.0%) capacitation (10.0 9.8 8.2% 12.4%) that similar > before after cryopreservation. Positive correlations found between versus (r = .63), .67) .88). In conclusion, alpaca is related a decrease motility, MMP, meanwhile are not affected.

Language: Английский

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Freezability biomarkers in the epididymal spermatozoa of swamp buffalo

Cryobiology, Journal Year: 2022, Volume and Issue: 106, P. 39 - 47

Published: May 1, 2022

Language: Английский

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Review: The role of male reproductive tract secretions in ruminant fertility

animal, Journal Year: 2023, Volume and Issue: 17, P. 100773 - 100773

Published: May 1, 2023

Male fertility largely depends on the ability to produce sperm that can transmit paternal information onto next generation. However, factors are critical for function and subsequent development of healthy offspring still not completely understood in ruminants. Importantly, is encoded by germ cell DNA, but rather, sequential acquisition, loss, modification elements through interaction with secretions from testes, epididymides, accessory glands (collectively termed seminal plasma). In addition, these play a role inheritance environmental effects progeny. This likely achieved directly, regulation epigenetic effectors, indirectly, altering female environment which individual develops. review will provide an overview different organs contribute plasma ruminants, summarise how their shape modulate reproductive tract. Finally, some consideration be given potential affect embryo health

Language: Английский

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Review: Sperm cryopreservation in wild small ruminants: morphometric, endocrine and molecular basis of cryoresistance

animal, Journal Year: 2023, Volume and Issue: 17, P. 100741 - 100741

Published: May 1, 2023

Reproductive technologies can help to protect wild ruminant species from becoming extinct. In addition, the decline in some game has also raised interest reproductive increase number of animals that be produced. Most biobanking efforts have focused on developing effective protocols for preserving sperm, oocytes, and embryos. Cryopreservation sperm remains least invasive method cheapest procedure germplasm storage. Over last few years, several biotechnologies been developed beyond conventional freezing spermatozoa. These include ultra-rapid techniques. Nevertheless, fertility results after artificial insemination using frozen-thawed spermatozoa are not always acceptable small ruminants. Moreover, these technological met variable success related sample's origin (epididymal retrieved postmortem or ejaculated) season sample collection Epididymal shows higher cryoresistance than ejaculated sperm. Changes proteome between epididymal seem contribute this different cryotolerance. The role endocrine status studied better understand underlying mechanism annual variation cryoresistance. Seasonal changes testosterone prolactin involved cryoresistance; recovery cryopreservation recommended around end rutting season, when good quality samples still obtained, levels already decreased, concentrations remain low. mechanisms hormone action freezability well known. Still, it suggested affects cell proliferation testis, during spermatogenesis, membrane properties cells their transit through tract, which might influence Recent studies revealed expression aquaporins ruminants could androgen-related seasonal Along with spermatozoa, testicular tissue may provide a suitable source male gametes, an alternative establishing banks semen cannot collected whatever reason.

Language: Английский

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Location of aquaporins 3, 7 and 10 in frozen-thawed ejaculated and cauda epididymal spermatozoa from the Iberian ibex, mouflon, and chamois

Theriogenology Wild, Journal Year: 2023, Volume and Issue: 2, P. 100025 - 100025

Published: Jan. 1, 2023

Spermatozoa collected from the cauda epididymis of wild ruminants are more cryoresistant than ejaculated spermatozoa. Changes in membrane location aquaporins (AQPs) follow osmotic changes that occur during freeze-thawing, and might influence cryosurvival spermatozoa depending on their source. This work reports AQP3, AQP7 AQP10 epididymal post-ejaculation three mountain ungulate species (Iberian ibex, mouflon, chamois), as determined by Western blotting (WB) immunocytochemistry (ICC) using commercial rabbit polyclonal primary antibodies. WB confirmed presence all AQPs studied species, while ICC showed AQP3 to be mainly located sperm acrosome, mid-piece, principal piece, end both cells. The percentage showing piece was higher ibex chamois (P < 0.05), mouflon 0.05). acrosome spermatozoa, well cytoplasmic droplet species. No differences were seen between with respect AQP7. tail In conclusion, except for locations similar inter-species only AQP3. Further studies needed determine what this mean cryopreservation.

Language: Английский

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Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model

Andrology, Journal Year: 2021, Volume and Issue: 9(4), P. 1242 - 1253

Published: March 9, 2021

Abstract Background Recent studies have noted that the circulating testosterone concentration may affect ability of spermatozoa to survive cryopreservation. However, few attempts confirm such a relationship been made. Wild ruminant species very marked seasonal changes in their reproductive function and strong annual plasma concentration. Objectives The present work examines influence induced secretion on sperm variables following conventional slow freezing ultra‐rapid freezing, using Iberian ibex as an experimental model. Materials Methods In first experiment, levels were reduced middle rutting season (December) antiandrogen cyproterone acetate (CA). second increased at end (January) via use androgen propionate (TP). Results During December, was found be higher blood seminal untreated males than those CA‐treated ( p < 0.001 0.05, respectively). Compared with controls, TP‐treated animals had concentrations but lower during January 0.01 0.001, vesicles larger 0.05). When CA administered, viability improved compared controls 0.05), irrespective protocol followed. For procedure, cryoresistance ratio for motility decreased when TP administered values fresh morphometric 50 days after treatment 0.001) over same time 0.001). Discussion Conclusion appears cryoresistance. This explain seen freezability some species, independent quality.

Language: Английский

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Current perspectives on ruminant sperm freezability: Harnessing molecular changes related to semen quality through omics technologies

Veterinary Integrative Sciences, Journal Year: 2021, Volume and Issue: 19(3), P. 487 - 511

Published: May 6, 2021

The recent advances in sperm cryopreservation transcend cryobanking and other assisted reproductive technologies. Since its discovery, has contributed positive impacts on animal breeding as well genetic exchange, improvement, conservation efforts. However, cryoinjury variabilities outcomes remain key challenges to cryobiology. present work explored the molecular bases for such freezability differences freezing-thawing injuries ruminant sperm. Relevant biomarkers identified seminal plasma spermatozoa were highlighted, including lipids, proteins, metabolites, transcripts, genes. Specific mechanisms concerning structures functions also examined relative their association cryotolerance, spermiogram or seminogram modifications following procedures. Current conflicts gaps knowledge base emphasized. Further investigation of these areas using available breakthrough tools omics technologies is therefore proposed improve, optimize, even predict overall quality frozen-thawed semen towards efficiency.

Language: Английский

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