Improving wild bee monitoring, sampling methods, and conservation

Basic and Applied Ecology, Journal Year: 2024, Volume and Issue: 75, P. 2 - 11

Published: Jan. 19, 2024

Bees are the most important group of insect pollinators, but their populations declining. To gain a better understanding wild bee responses to different stressors (e.g. land-use change) and conservation measures, regional national monitoring schemes currently being established in Germany, which is used here as model region, many other countries. We offer perspectives on how best design future programs with focus evaluating implementation measures. discuss traditional novel sampling methods, efficacy depending research questions life-history traits target species, greater standardization methods can make data more comparable, contributing identification general trends mechanisms driving populations. Furthermore, potential impact itself discussed.

Language: Английский

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Barcoding the Caatinga biome bees: a practical review

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Feb. 4, 2025

Abstract Bees play a critical role as pollinators in ecosystem services, contributing significantly to the sexual reproduction and diversity of plants. The Caatinga biome Brazil, home around 200 bee species, provides an ideal habitat for these species due its unique climate conditions. However, this faces threats from anthropogenic processes, making it urgent characterise local populations efficiently. Traditional taxonomic surveys identification are complex lack suitable keys expertise required. As result, molecular barcoding has emerged valuable tool, using genome regions compare identify species. little is known about bees develop tools further. This study addresses gap, providing updated list 262 across 86 genera identifying ~ 40 primer sets aid findings highlight ongoing work needed fully biome’s distribution or subspecies support more effective monitoring conservation efforts.

Language: Английский

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Taxonomic revision of the Stenodynerus fastidiosissimus species-group in Western Europe and North Africa (Hymenoptera: Vespidae: Eumeninae)

Zootaxa, Journal Year: 2024, Volume and Issue: 5418(1), P. 34 - 56

Published: Feb. 26, 2024

The fastidiosissimus species-group of Stenodynerus de Saussure, 1863 is revised in Western Europe and North Africa, combining morphological data DNA barcoding. Six species are recognized: S. difficilis (Morawitz, 1867) stat. resurr. (= auct.), (de 1855), laborans (Costa, 1882) resurr., montanus Selis, sp. nov., muelleri (Dusmet, 1917) gusenleitneri Giordani Soika, 1986 syn. nov.), rufescens 1977 nov. Lectotypes designated for Odynerus 1855 insularis André, 1883 non Smith, 1859. A key the identification members this provided. barcodes published every species, representing first available sequences species-group.

Language: Английский

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The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Bees

Biodiversity Data Journal, Journal Year: 2024, Volume and Issue: 12

Published: March 5, 2024

Bees are important actors in terrestrial ecosystems and recognised for their prominent role as pollinators. In the Iberian Peninsula, approximately 1,100 bee species known, with nearly 100 of these being endemic to Peninsula. A reference collection DNA barcodes, based on morphologically identified specimens, representing 514 species, was constructed. The "InBIO Barcoding Initiative Database: Barcodes bees" dataset contains records 1,059 sequenced specimens. this correspond about 47% diversity 21% diversity. For peninsular Portugal only, corresponding coverage is 71% 50%. Specimens were collected between 2014 2022 deposited research Thomas Wood (Naturalis Biodiversity Center, Netherlands), FLOWer Lab at University Coimbra (Portugal), Andreia Penado Natural History Science Museum Porto (MHNC-UP) (Portugal) InBIO (IBI) (Vairão, Portugal). Of sequenced, 75 from five different families new additions Barcode Life Data System (BOLD) 112 BINs added. Whilst majority assigned a single BIN (94.9%), 27 nominal multiple BINs. Although placement into may simply reflect genetic variation, it likely also represents currently unrecognised species-level across diverse taxa, such Amegilla albigena Lepeletier, 1841, Andrena russula Lasioglossum leucozonium (Schrank, 1781), Nomada femoralis Morawitz, 1869 Sphecodes alternatus Smith, 1853. Further pairs Colletes , Hylaeus placed same BINs, emphasising need integrative taxonomy within Iberia Mediterranean Basin more broadly. These data substantially contribute our understanding barcodes provide an baseline ongoing taxonomic revisions West Palaearctic biogeographical region.

Language: Английский

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DNA barcoding insufficiently identifies European wild bees (Hymenoptera, Anthophila) due to undefined species diversity, genus‐specific barcoding gaps and database errors

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: 24(5)

Published: March 25, 2024

Abstract Recent declines in insect abundances, especially populations of wild pollinators, pose a threat to many natural and agricultural ecosystems. Traditional species monitoring relies on morphological character identification is inadequate for efficient standardized surveys. DNA barcoding has become standard approach molecular organisms, aiming overcome the shortcomings traditional biodiversity monitoring. However, its efficacy depends completeness reference databases. Large efforts are (almost entirely) lacking European countries such patchy data limit Europe‐wide analyses precisely how apply bee identification. Here, we advance towards an effective bees. We conducted high‐effort survey bees at junction central southern Europe barcoded all collected morphospecies. For global analyses, complemented our barcode dataset with relevant delimitation, general genus‐specific gaps examined error rate repositories. found that (i) sixth specimens from Slovenia could not be reliably identified, (ii) delimitation methods show numerous systematic discrepancies, (iii) there no gap across (iv) genus specific, but only after curating errors Intense sampling underrepresented regions strict curation repositories needed enhance use

Language: Английский

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The genus Euodynerus Dalla Torre in Europe and the Maghreb (Hymenoptera: Vespidae: Eumeninae)

Zootaxa, Journal Year: 2024, Volume and Issue: 5537(2), P. 151 - 194

Published: Nov. 7, 2024

The genus Euodynerus Dalla Torre, 1904 (= Extraepipona Gusenleitner, 2014, syn. nov.; occultus (Gusenleitner, 2014), comb. nov.) is revised in Europe and the Maghreb, combining morphological data DNA barcoding. New synonymies are proposed for E. (Pareuodynerus) Blüthgen, 1938 (Incolepipona) Giordani Soika, 1994, nov.), annae (Kostylev, 1937) shirazensis 1970, caspicus (Morawitz, 1873) armeniacus 2016, curictensis 1940 sardous Borsato, 2006, dantici (Rossi, 1790) poggii 1986, = minoricensis Sanza, 2003, quadrifasciatus (Fabricius, 1793) atripes 1976, rufipes 1984, eburnus Yamane, 1987, rubrosignatus stat. nov. notatus cyrenaicus variegatus Odynerus crenatus kruegeri von Schulthess, 1928, nov.). 1984 raised to species-level (E. rubrosignatus, bidentoides (Giordani 1953), sp. resurr. removed from synonymy with bidentiformis 1942). bidentatus (Lepeletier, 1841) transferred subgenus Pareuodynerus s. str. A key identification of Euro-Maghrebi species reference photos each provided.

Language: Английский

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Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France

Biodiversity Data Journal, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 7, 2025

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on resolution sequences used as barcodes reliability reference databases. For wild bees, barcode coverage is consistently growing in volume, but some incorrect species annotations need be cared for. The COI (Cytochrome Oxydase subunit 1) gene, most barcoding/metabarcoding arthropods, suffers from primer bias difficulties covering all bee using classical Folmer primers. We present here a curated database 250 bp mini-barcode region 16S rRNA suitable low-cost bees applications, such eDNA analysis or sequencing ancient degraded DNA. Sequenced specimens were captured Occitania (south-west France) morphologically identified by entomologists, with total 530 individuals 171 19 genera. A customised workflow including distance-tree inferences second round entomologist observations, when necessary, was validation 348 mini-barcodes 148 species. Amongst them, 93 did not have any available before our contribution. This high-quality library data freely scientific community, aim facilitating future large-scale characterisation communities context pollinators' decline.

Language: Английский

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Integrating taxonomic, genetic and ecological data to explore the species richness of wild bees (Hymenoptera, Apoidea, Anthophila) of the Culuccia Peninsula (NE Sardinia, Italy)

Journal of Hymenoptera Research, Journal Year: 2025, Volume and Issue: 98, P. 117 - 145

Published: Feb. 10, 2025

Wild bees are essential pollinators of both native and cultivated plants, but their populations declining worldwide. Conservation efforts hindered by insufficient data, especially in the Mediterranean basin, which hosts some most diverse pollinator communities world. Particularly Sardinia, second largest island Mediterranean, information on bee fauna is still limited. The aim this work was to provide first checklist ApoideaAnthophila from an unexplored peninsula north-eastern Sardinia (Italy), combining traditional (morphologically-based) taxonomy DNA barcoding. In addition, records flower visits provided shown a visitor network enrich scarce data associations between wild plants Region. Bees were sampled April October 2022–2023 with two Malaise traps nets. extracted amplify sequences mitochondrial gene Cyotochrome oxydase I, then compared those BOLD using identification tool constructing neighbor-joining phylogenetic trees. Seventy-six different species belonging 29 genera six families collected identified. A total 212 COI obtained for 61 species, many had not yet been sequenced Italian populations. Five taxa Sardo-Corsican endemics, whereas newly recorded Sardinia. Finally, we highlight potential taxonomic issues new visit records, emphasizing need further research better understand ecology group insects toward conservation.

Language: Английский

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New distribution records of wild bees (Hymenoptera, Apoidea) in South Tyrol (Italy): expanding the wild bee database

Biodiversity Data Journal, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 25, 2025

Throughout South Tyrol, in northern Italy, there is a data deficiency relating to wild bee species pool. Here, we present significant findings from the collection of 3,313 bees gathered over two separate studies conducted consecutive years. Our research focused on impact landscape heterogeneity, temperature and land-use change communities their pollination services an agricultural mountainous landscape. This article provides detailed account 150 identified collected using coloured pan traps. We report habitat type, occurrence data, threat status, sociality, nesting strategy diet breadth. In Italian regions where information distribution lacking or outdated, sharing crucial for developing conservation policies. The compiled list strengthens regional national database by providing new extinction-threatened species, such as Dufoureadentiventris (Nylander, 1848), Dufoureainermis Lasioglossumbrevicorne (Schenck, 1870), Lasioglossumlaevigatum (Kirby, 1802), Lasioglossummonstrificum (Morawitz, 1891), Nomadamutica Morawitz, 1872 Nomadavillosa Thomson, 1870. Additionally, recent that are valuable understanding range expansions, recording previously unreported Tyrol updating historical region.

Language: Английский

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Molecular Identification of Wild Bees

Published: Jan. 1, 2025

Language: Английский

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