Genomic dynamics of high-risk carbapenem-resistant klebsiella pneumoniae clones carrying hypervirulence determinants in Egyptian clinical settings DOI Creative Commons
Nehal Adel Abdelsalam, Shahira A. ElBanna, Shaimaa F. Mouftah

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 22, 2024

Abstract Background Ongoing studies have revealed the global prevalence of severe infections caused by hypervirulent strains Klebsiella pneumoniae (K. pneumoniae) . Meanwhile, World Health Organization and Centers for Disease Control declared carbapenem-resistant K. as an urgent public health threat, requiring swift effective action to mitigate its spread. Low- middle-income countries are severely impacted such devastating infectious diseases owing ill implementation antimicrobial practices infection control policies. Having both hypervirulence carbapenemase gene determinants, emergence convergent is now being reported worldwide. Methods In this study, we sequenced 19 carbapenemase-producing recovered from various clinical specimens. Additionally, evaluated phenotypic susceptibility multiple classes using VITEK2 automated system. Utilizing sequencing data, characterized sequence types, serotypes, pangenome, resistance profiles, virulence mobile genetic elements examined isolates. We highlighted high-risk clones carrying determinants among screened Results Our findings that all isolates exhibited either extensive- or pan-drug harbored variants genes spanning nearly classes. The most prevalent detected within were bla NDM−5 OXA−48 identified clones, ST383-K30, ST147-K64, ST11-K15, ST14-K2, which may evolved into putative acquiring full set hypervirulence-associated ( iucABCD , rmpA and/ rmpA2 transporter peg-344 ). study ST709-K9 a clone first time uncovered capsule types K15 K9 carried determinants. frequent Inc found in these Col440I, IncHI1B, FII(K). Conclusion This highlights high-risk, extensively co-carrying Egyptian settings. poses imminent threat not only Egypt but also community, underscoring need enhanced surveillance strategies combat pathogen.

Language: Английский

Longitudinal profiling of the microbiome at four body sites reveals core stability and individualized dynamics during health and disease DOI Creative Commons
Xin Zhou, Xiaotao Shen, Jethro S. Johnson

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(4), P. 506 - 526.e9

Published: March 12, 2024

To understand the dynamic interplay between human microbiome and host during health disease, we analyzed microbial composition, temporal dynamics, associations with multi-omics, immune, clinical markers of microbiomes from four body sites in 86 participants over 6 years. We found that stability individuality are body-site specific heavily influenced by host. The stool oral more stable than skin nasal microbiomes, possibly due to their interaction environment. identify individual-specific commonly shared bacterial taxa, individualized taxa showing greater stability. Interestingly, dynamics correlate across sites, suggesting systemic host-microbial-environment interactions. Notably, insulin-resistant individuals show altered among microbiome, molecular markers, features, disrupted metabolic disease. Our study offers comprehensive views multi-site relationship

Language: Английский

Citations

49
Vaccine value profile for Klebsiella pneumoniae DOI Creative Commons
Ziyaad Dangor, Nicole M. Benson, James A. Berkley

et al.

Vaccine, Journal Year: 2024, Volume and Issue: 42(19), P. S125 - S141

Published: March 19, 2024

Klebsiella pneumoniae causes community- and healthcare-associated infections in children adults. Globally 2019, an estimated 1.27 million (95% Uncertainty Interval [UI]: 0.91-1.71) 4.95 UI: 3.62-6.57) deaths were attributed to associated with bacterial antimicrobial resistance (AMR), respectively. K. was the second leading pathogen AMR resistant bacteria. Furthermore, rise of both hospital-acquired is a concern for neonates infants who are at high risk invasive disease. There limited antibiotic pipeline new antibiotics treat multidrug infections, vaccines targeted against considered be priority by World Health Organization. Vaccination pregnant women could reduce K.pneumoniae disease their young offspring. In addition, vulnerable children, adolescents adult populations underlying diseases such as immunosuppression from hematologic malignancy, chemotherapy, patients undergoing abdominal and/or urinary surgical procedures, or prolonged intensive care management also potential target groups vaccine. A 'Vaccine Value Profile' (VVP) K.pneumoniae, which contemplates vaccination protect babies birth through least three months age other high-risk populations, provides high-level, holistic assessment available information inform public health, economic societal value preventatives therapeutics. This VVP developed working group subject matter experts academia, non-profit organizations, public-private partnerships, multi-lateral collaboration stakeholders WHO. All contributors have extensive expertise on various elements collectively aimed identify current research knowledge gaps. The using only existing publicly information.

Language: Английский

Citations

22
Private and well drinking water are reservoirs for antimicrobial resistant bacteria DOI Creative Commons
Marwa Alawi, Cian Smyth, David Drissner

et al.

npj Antimicrobials and Resistance, Journal Year: 2024, Volume and Issue: 2(1)

Published: March 18, 2024

Abstract Water quality testing does not recognise antimicrobial resistance (AMR) and is often limited to indicators of faecal contamination Escherichia coli Enterococcus species. In Europe, data on AMR in drinking water scarce. Ireland, as many countries, household supplied via mains or private wells schemes. Using citizen science, we identified Irish supplies reservoirs resistant bacteria (ARB). Gram-negative ( n = 464) Gram-positive 72) were isolated. We instances potentially opportunistic ARB such Enterobacter cloacae, Acinetobacter baumannii report multidrug casseliflavus, E. coli, Stenotrophomonas maltophilia , Serratia rubidaea . also linezolid-resistant water. Linezolid a last-resort antibiotic used treat vancomycin-resistant sp. Additionally, mobile three samples, two which carried IncF group, one IncQ five Col-like plasmids. Our work suggests that potential sink source pathogens. This highlights value surveillance One Health framework the would provide information regarding movement persistence ARGs are able survive subsequently have opportunity be mobilised through humans; linking environment human threatening health.

Language: Английский

Citations

10
Ecological and evolutionary mechanisms driving within-patient emergence of antimicrobial resistance DOI
Matthew J. Shepherd, Taoran Fu, Niamh E. Harrington

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(10), P. 650 - 665

Published: April 30, 2024

Language: Английский

Citations

9
Genomic features of Klebsiella isolates from artisanal ready-to-eat food production facilities DOI Creative Commons
Cecilia Crippa, Frédérique Pasquali, Carla Rodrigues

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 6, 2023

Abstract Increasing reports on K. pneumoniae strains with antimicrobial resistance and virulence traits from food farm animals are raising concerns about the potential role of Klebsiella spp. as a foodborne pathogen. This study aimed to report characterize isolates two artisanal ready-to-eat (soft cheese salami) producing facilities, track similar genotypes in different ecological niches. Over 1170 samples were collected during whole production chain batches. The overall prevalence was 6%. Strains classified into three species complexes: (KpSC, n = 17), oxytoca (KoSC, 38) planticola (KplaSC, 18). Despite high genetic diversity we found terms known new sequence types (STs), core genome phylogeny revealed clonal persisting same processing setting for over 14 months, isolated environment, raw materials end-products. showed natural phenotype-genotype. highest potential, ST4242 ST107 carrying yersiniabactin ybt16 aerobactin iuc3 . latter detected all salami located large conjugative plasmid highly (97% identity) iuc 3 + plasmids human pig circulating nearby regions Italy. While identical may persist along process, distinct sources facility shared an 3-plasmid. Surveillance will be crucial obtain more comprehensive picture circulation pathogenic potential.

Language: Английский

Citations

12
Treated wastewater: A hotspot for multidrug- and colistin-resistant Klebsiella pneumoniae DOI Creative Commons
Andrea Di Cesare, Alessandra Cornacchia, Tomasa Sbaffi

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 359, P. 124598 - 124598

Published: July 23, 2024

Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October, November 2018. We detected characterized 42 K. strains using whole genome sequencing (15 8 19 effluent). Additionally, were tested their resistance phenotype. Using no distinct patterns observed terms genetic profiles. All tetracycline, meanwhile 60%, 47%, 37.5% isolated effluent, respectively, multidrug resistant. Some isolates also colistin, nearly all positive eptB arnT genes, associated with polymyxin Various genes linked mobile elements, they did not correlate virulence groups or defense systems. Overall, our results, although quantitative, highlight that strains, including those colistin genetically unrelated, being discharged ecosystems plants. This suggests necessity monitoring aimed at characterizing these bacteria.

Language: Английский

Citations

4
ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas DOI Creative Commons

Samanta Freire,

Teresa Grilo,

Bruna dos Santos Rodrigues

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(2), P. 415 - 415

Published: Feb. 7, 2023

Bivalves are filter-feeding organisms and biomarkers of bacterial pollution. Our study aimed to analyze the occurrence characteristics extended-spectrum β-lactamase (ESBL)- carbapenemase-producing Escherichia coli among bivalves. A total 522 bivalve samples were collected along Portuguese shellfish production areas. Homogenized screened for E. contamination on corresponding selective plates, allowing concomitant growth Klebsiella pneumoniae. was observed in 39% samples. Subsequent screening identified nine (4.4%) contaminated with ESBL producers, (n = 7) K. pneumoniae 2), while a single (0.5%) identified. ESBLs all CTX-M-types commonly human isolates, i.e., CTX-M-32 4), CTX-M-15 CTX-M-14 1). The carbapenemase producer harbored blaGES-5 gene located ColE plasmid. Clonality evaluated by multilocus sequence typing, identifying backgrounds as ST10, ST23, ST540, ST617, ST746, SLV206, SLV2325, environmental strains. isolates belonged ST834, ST15, DLV644. ESBL- Enterobacteriaceae bivalves reveals how marine environment constitutes reservoir critical pathogens, thus potentially representing risk health.

Language: Английский

Citations

10
Evaluation of Potential Factors Influencing the Dissemination of Multidrug-Resistant Klebsiella pneumoniae and Alternative Treatment Strategies DOI Creative Commons
Thando Ndlovu, Lebang Kgosietsile, Pako Motshwarakgole

et al.

Tropical Medicine and Infectious Disease, Journal Year: 2023, Volume and Issue: 8(8), P. 381 - 381

Published: July 26, 2023

The increasing reports of multidrug-resistant Klebsiella pneumoniae have emerged as a public health concern, raising questions about the potential routes for evolution and dissemination pathogenic K. into environmental reservoirs. Potential drivers increased incidence antimicrobial-resistant include eminent global climatic variations direct or indirect effect human activities. ability microorganisms to adapt grow at an exponential rate facilitates distribution strains with acquired resistant mutations water systems, vegetation, soil which are major intersection points animals humans. bacterial pathogen, pneumoniae, is one critical-priority pathogens listed by World Health Organization, mostly associated hospital-acquired infections. However, prevalence similar characteristics clinical-antibiotic-resistant isolates concerning. Considering impact in spread bacteria, this review, we closely assess factors influencing pathogen resulting interaction environment, beings, animals. We also look recent developments rapid detection techniques part response measures improve surveillance preparedness outbreaks. Furthermore, discuss alternative treatment strategies that secondary metabolites such biosurfactants plant extracts high antimicrobial properties.

Language: Английский

Citations

10
Global dissemination of Klebsiella pneumoniae in surface waters: genomic insights into drug resistance, virulence, and clinical relevance DOI Creative Commons
Damian Rolbiecki, Edyta Kiedrzyńska, Małgorzata Czatzkowska

et al.

Drug Resistance Updates, Journal Year: 2025, Volume and Issue: 79, P. 101204 - 101204

Published: Jan. 21, 2025

Language: Английский

Citations

0
Harnessing Molecular and Bioactivity Network Analysis to Prioritize Antibacterial Compound Isolation From Ant‐Associated Fungi DOI Creative Commons
Ángel Sahid Aguilar-Colorado, Jesús Morales‐Jiménez, José Rivera‐Chávez

et al.

Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

ABSTRACT Introduction Antimicrobial resistance is a global public health problem that requires the development of new bioactive compounds. In this context, metabolomic analyses can expedite research fungal metabolites as valuable resource. Objectives To investigate metabolic profiles and isolate antibacterial compounds from micromycetes associated with Mexican cloud forest ants by utilizing network analysis their chemical bioactivity data. Material Methods 248 strains isolated six ant's species, soil anthills, surroundings were evaluated for in vitro inhibition growth extensively drug‐resistant Acinetobacter baumannii hypervirulent Klebsiella pneumoniae ; subsequently, dereplicated analyzed molecular networking compound activity mapping spectrometric Prioritization some fungi isolation major constituents was performed, structures established spectroscopic determined. Results From collection, 15 secondary ( 1 – ) dereplicated, 10 16 25 ), including E )‐tridec‐7‐ene‐3,5,6,10‐tetraol Ascomycetes Trichoderma , Cladosporium Clonostachys genera. Compounds 18 stood out being bioactive. This study first report against A. tricyclic pyridin‐2‐ones deoxy‐PF1140 PF1140 17 minimum inhibitory concentration 50 μg/mL. Conclusion Network dereplication proved effective bioprospecting compounds, offering insights into diversity potential applications. Moreover, broadens knowledge linked to leafcutter, fire, warrior ants.

Language: Английский

Citations

0