Roles of intralesional bacteria in the initiation and progression of oral squamous cell carcinoma DOI Creative Commons

Zhuoyan Luo,

Shiping Lv,

Fangzhi Lou

et al.

Cancer Medicine, Journal Year: 2024, Volume and Issue: 13(18)

Published: Sept. 1, 2024

Abstract Background Oral squamous cell carcinoma (OSCC) is the predominant form of head and neck cancer, often diagnosed at late stages, resulting in a poor prognosis. Recent studies indicate potential association between OSCC microbial presence. Microorganisms have been identified various tumors lesions, including oral potentially malignant disorders (OPMDs). Intralesional microbiota are considered important components tumor microenvironment (TME) may contribute to carcinogenesis. Methods Sources were collected through thorough searches databases PubMed Embase. The review focused on characteristics, origins, their impact cancer progression. Results Bacteria display varying abundance diversity throughout stages OPMDs. Intraleisional bacteria diverse sources, not only plaque saliva but also gut. both pro‐carcinogenic anti‐carcinogenic effects, affecting processes like proliferation, invasion, immune response. Conclusions crucial OPMDs, influencing disease progression treatments. Despite significance, challenges inconsistent sampling identification remain. Future research required fully understand role improve clinical applications.

Language: Английский

Oral streptococci S. anginosus and S. mitis induce distinct morphological, inflammatory, and metabolic signatures in macrophages DOI Creative Commons

Sangeetha Senthil Kumar,

Venugopal Gunda, Dakota M. Reinartz

et al.

Infection and Immunity, Journal Year: 2024, Volume and Issue: 92(3)

Published: Jan. 30, 2024

ABSTRACT Oral streptococci, key players in oral biofilm formation, are implicated dysbiosis and various clinical conditions, including dental caries, gingivitis, periodontal disease, cancer. Specifically, Streptococcus anginosus is associated with esophageal, gastric, pharyngeal cancers, while mitis linked to However, no study has investigated the mechanistic links between these species cancer-related inflammatory responses. As an initial step, we probed innate immune response triggered by S. RAW264.7 macrophages. These bacteria exerted time- dose-dependent effects on macrophage morphology without affecting cell viability. Compared untreated macrophages, macrophages infected exhibited a robust proinflammatory characterized significantly increased levels of cytokines mediators, TNF, IL-6, IL-1β, NOS2, COX2, accompanied enhanced NF-κB activation. In contrast, -infected failed elicit response. Seahorse Xfe96 analysis revealed extracellular acidification rate compared . At 24-h time point, presence led reduced itaconate, itaconate levels, highlighting distinct metabolic profiles during infection contrast aconitate decarboxylase expression observed at 6-h point. This investigation highlights how , two Gram-positive from same genus, can prompt responses shifts infection. IMPORTANCE The surge head neck cancer cases among individuals devoid typical risk factors such as Human Papilloma Virus (HPV) tobacco alcohol use sparks argumentative discussion around emerging role microbiota novel factor squamous carcinoma (OSCC). While substantial research dissected gut microbiome’s influence physiology, microbiome, notably been underappreciated mucosal immunopathogenesis. viridans streptococci group, abscess formation elevated esophageal OSCC. current aims probe early colonizer important first step toward understanding impact host response, which understudied determinant OSCC development progression.

Language: Английский

Citations

8
Age‐Specific Functional Connectivity Changes After Partial Sleep Deprivation Are Correlated With Neurocognitive and Molecular Signatures DOI Creative Commons
Liyong Yu, Xuanyi Chen, Yuqi He

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Feb. 1, 2025

This study aimed to investigate age-specific alterations in functional connectivity after sleep deprivation (SD) and decode brain changes from neurocognitive transcriptomic perspectives. Here, we examined global regional graph measures, particularly network strength (RNS), 41 young participants 36 older with normal 3 h of SD. Additionally, by utilizing cognitive probabilistic maps Neurosynth gene expression data the Allen Human Brain Atlas, applied partial least-squares regression analysis identify transcriptional correlates these RNS changes. After SD, exhibited decreased default mode (DMN) dorsal attention network, increased visual network. Young also showed DMN, notably left inferior parietal lobe, dorsolateral prefrontal cortex, posterior cingulate cortex. In participants, SD-induced significantly correlated processes such as "attention," "cognitive control," "working memory," while they "learning," "focus," "decision." Gene-category enrichment indicated that specific genes related signal transduction, ion channels, immune signaling might influence SD pathophysiology affecting participants. elucidates shared associated providing a molecular basis for understanding underlying pathophysiology.

Language: Английский

Citations

0
Streptococcus anginosus Activates the NLRP3 Inflammasome to Promote Inflammatory Responses from Macrophages DOI
Anika M. Arias, Dakota M. Reinartz,

Chloe Sairs

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Chronic inflammation and oral dysbiosis are common features of squamous cell carcinoma (OSCC). The commensal streptococci, S. anginosus, is increased in diseases including OSCC. Our previous work revealed that anginosus promotes inflammatory responses from macrophage lines, however the molecular mechanism by which interacts with macrophages to instigate this response remains be investigated. Here, we expand on our findings investigating effects infection primary bone marrow derived (BMMs) during vivo infection. We found activated BMMs, presented an enlarged cellular area, NF-κB activation downstream cytokines TNF⍰, IL-6 IL-1β at 24 hours post viability was dispensable for activation, but essential induction proteins cytokines. persisted intracellularly within BMMs induced expression inflammasome sensors AIM2, NLRC4 NLRP3. Further, lacking adapter protein ASC ( Asc -/- ) had significantly diminished production compared wild type indicating inflammasome. primarily triggered through NLRP3 as -infected Nlrp3 inhibitor (MCC950)-treated displayed controls. Lastly, mice reduced weight loss C57BL/6 mice. These overall indicate replicates a proinflammatory part brief summary sentence: sensed promote response.

Language: Английский

Citations

0
Streptococcal peptides and their roles in host-microbe interactions DOI Creative Commons

Emily R. Wahlenmayer,

Daniel E. Hammers

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Oct. 16, 2023

The genus Streptococcus encompasses many bacterial species that are associated with hosts, ranging from asymptomatic colonizers and commensals to pathogens a significant global health burden. Streptococci produce numerous factors enable them occupy their host-associated niches, of which alter host environment the benefit bacteria. ability manipulate immune systems either evade detection clearance or induce hyperinflammatory state influences whether bacteria able survive persist in given environment, while also influencing propensity cause disease. Several contribute this inter-species interaction have been identified. Recently, small peptides become increasingly appreciated as Streptococcal relationships hosts. Peptides utilized by streptococci modulate several ways, including directly interacting disrupt system function signaling other control expression genes modulation. In review, we discuss contributions terms pathogenesis disruption immunity. This discussion will highlight importance continuing elucidate functions these pursuing identification new modulation environments. Developing greater understanding how interact hosts has potential development techniques inhibit therapeutic approaches against infections.

Language: Английский

Citations

3
Streptolysin S induces proinflammatory cytokine expression in calcium ion-influx-dependent manner DOI Creative Commons

Yugo Yamamori,

Rina Shirai,

Kazuto Ohkura

et al.

Current Research in Microbial Sciences, Journal Year: 2024, Volume and Issue: 7, P. 100265 - 100265

Published: Jan. 1, 2024

Anginosus group streptococci (AGS) are opportunistic pathogens that reside in the human oral cavity. The β-hemolytic strains of

Language: Английский

Citations

0
Roles of intralesional bacteria in the initiation and progression of oral squamous cell carcinoma DOI Creative Commons

Zhuoyan Luo,

Shiping Lv,

Fangzhi Lou

et al.

Cancer Medicine, Journal Year: 2024, Volume and Issue: 13(18)

Published: Sept. 1, 2024

Abstract Background Oral squamous cell carcinoma (OSCC) is the predominant form of head and neck cancer, often diagnosed at late stages, resulting in a poor prognosis. Recent studies indicate potential association between OSCC microbial presence. Microorganisms have been identified various tumors lesions, including oral potentially malignant disorders (OPMDs). Intralesional microbiota are considered important components tumor microenvironment (TME) may contribute to carcinogenesis. Methods Sources were collected through thorough searches databases PubMed Embase. The review focused on characteristics, origins, their impact cancer progression. Results Bacteria display varying abundance diversity throughout stages OPMDs. Intraleisional bacteria diverse sources, not only plaque saliva but also gut. both pro‐carcinogenic anti‐carcinogenic effects, affecting processes like proliferation, invasion, immune response. Conclusions crucial OPMDs, influencing disease progression treatments. Despite significance, challenges inconsistent sampling identification remain. Future research required fully understand role improve clinical applications.

Language: Английский

Citations

0