Current Opinion in Genetics & Development, Journal Year: 2024, Volume and Issue: 87, P. 102207 - 102207
Published: May 30, 2024
Language: Английский
International Journal of Medical Sciences, Journal Year: 2023, Volume and Issue: 20(8), P. 1079 - 1090
Published: Jan. 1, 2023
N4-acetylcytidine (ac4C) is a lately discovered nucleotide modification that has been shown to be closely implicated in cancer.N-acetyltransferase10(NAT10) acts as an enzyme regulates mRNA acetylation modifications.Currently, the role of NAT10-mediated RNA cervical cancer remains elucidated.On basis transcriptome analysis TCGA and GEO open datasets (GSE52904, GSE29570, GSE122697), NAT10 upregulated tissues correlated with poor prognosis.Knockdown suppressed cell proliferation, invasion, migration cells.The vivo oncogenic function was also confirmed xenograft models.Combined RNA-seq acRIP-seq revealed HNRNPUL1 target cancer.NAT10 positively regulate expression by promoting ac4C stability mRNA.Furthermore, depletion division, cancer.HNRNPUL1 overexpression partially restored cellular cells knockdown.Thus, this study demonstrates contributes progression enhancing via modification, NAT10-ac4C-HNRNPUL1 axis might potential for therapy.
Language: Английский
Citations
22
Seminars in Immunology, Journal Year: 2023, Volume and Issue: 69, P. 101809 - 101809
Published: July 19, 2023
Language: Английский
Citations
22
Pharmacology & Therapeutics, Journal Year: 2023, Volume and Issue: 253, P. 108576 - 108576
Published: Dec. 7, 2023
Language: Английский
Citations
22
Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(11)
Published: Nov. 1, 2023
Abstract N4-acetylcytidine (ac4C) is a post-transcriptional RNA modification that regulates in various important biological processes. However, its role human cancer, especially lymph node metastasis, remains largely unknown. Here, we demonstrated N-Acetyltransferase 10 (NAT10), as the only known “writer” of ac4C mRNA modification, was highly expressed head and neck squamous cell carcinoma (HNSCC) patients with metastasis. High NAT10 levels nodes HNSCC are predictor poor overall survival. Moreover, found high expression positively upregulated by Nuclear Respiratory Factor 1 (NRF1) transcription factor. Gain- loss-of-function experiments displayed promoted metastasis mice. Mechanistically, induced Glycosylated Lysosomal Membrane Protein (GLMP) stabilized mRNA, which triggered activation MAPK/ERK signaling pathway. Finally, NAT10-specific inhibitor, remodelin, could inhibit tumorigenesis 4-Nitroquinoline 1-oxide (4NQO)-induced murine tumor model remodel microenvironment, including angiogenesis, CD8 + T cells Treg recruitment. These results demonstrate promotes via ac4C-dependent stabilization GLMP transcript, providing potential epitranscriptomic-targeted therapeutic strategy for HNSCC.
Language: Английский
Citations
21
Advanced Science, Journal Year: 2023, Volume and Issue: 11(8)
Published: Dec. 3, 2023
Abstract Neutrophils, accounting for ≈70% of human peripheral leukocytes, are key cells countering bacterial and fungal infections. Neutrophil homeostasis involves a balance between cell maturation, migration, aging, eventual death. Neutrophils undergo different death pathways depending on their interactions with microbes external environmental cues. has significant physiological implications leads to distinct immunological outcomes. This review discusses the multifarious neutrophil pathways, including apoptosis, NETosis, pyroptosis, necroptosis, ferroptosis, outlines effects immune responses disease progression. Understanding multifaceted aspects death, intersections among signaling ramifications immunity will help facilitate development novel therapeutic methods.
Language: Английский
Citations
20
Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)
Published: Jan. 17, 2024
Abstract N 4 -acetylcytidine (ac C) is a highly conserved chemical modification widely found in eukaryotic and prokaryotic RNA, such as tRNA, rRNA, mRNA. This significantly associated with various human diseases, especially cancer, its formation depends on the catalytic activity of N-acetyltransferase 10 (NAT10), only known protein that produces ac C. review discusses detection techniques regulatory mechanisms C summarizes correlation tumor occurrence, development, prognosis, drug therapy. It also comments new biomarker for early diagnosis prognosis prediction target
Language: Английский
Citations
8
BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)
Published: April 30, 2024
Periodontitis is a chronic osteolytic inflammatory disease, where anti-inflammatory intervention critical for restricting periodontal damage and regenerating alveolar bone. Ropinirole, dopamine D2 receptor agonist, has previously shown therapeutic potential periodontitis but the underlying mechanism still unclear.
Language: Английский
Citations
8
Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 256, P. 155224 - 155224
Published: Feb. 23, 2024
Language: Английский
Citations
6
International Journal of Biological Sciences, Journal Year: 2023, Volume and Issue: 20(1), P. 152 - 174
Published: Nov. 15, 2023
The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) is a crucial innate defence mechanism against viral infection in the immune system, as it principally induces production type I interferons. Immune responses and metabolic control are inextricably linked, chronic low-grade inflammation promotes development diseases. cGAS-STING pathway activated by double-stranded DNA (dsDNA), dinucleotides (CDNs), endoplasmic reticulum stress (ER stress), mitochondrial stress, energy imbalance cells triggers proinflammatory disorders. Abnormal overactivation closely associated with diseases such obesity, nonalcoholic fatty liver disease (NAFLD), insulin resistance cardiovascular (CVDs). interaction other pathways, nuclear factor-kappa B (NF-κB), Jun N-terminal kinase (JNK), AMP-activated protein (AMPK), mammalian target rapamycin (mTOR), autophagy, pyroptosis signalling considered an important which regulates metabolism. This review focuses on link between related to pathways for mediating signal input affecting output. Moreover, potential inhibitors therapeutic prospects discussed. provides comprehensive perspective involvement STING immune-related
Language: Английский
Citations
16
Antioxidants, Journal Year: 2023, Volume and Issue: 12(5), P. 1116 - 1116
Published: May 18, 2023
Recently, we reported that N-acetyltransferase 10 (NAT10) regulates fatty acid metabolism through ac4C-dependent RNA modification of key genes in cancer cells. During this work, noticed ferroptosis as one the most negatively enriched pathways among other NAT10-depleted In current explore possibility whether NAT10 acts an epitranscriptomic regulator pathway Global ac4C levels and expression with ferroptosis-related were assessed via dotblot RT-qPCR, respectively. Flow cytometry biochemical analysis used to assess oxidative stress features. The ac4C-mediated mRNA stability was conducted using RIP-PCR assay. Metabolites profiled LC-MS/MS. Our results showed significant downregulation essential related ferroptosis, namely SLC7A11, GCLC, MAP1LC3A, SLC39A8 Further, a reduction cystine uptake reduced GSH levels, along elevated ROS, lipid peroxidation Consistently, overproduction oxPLs, well increased mitochondrial depolarization decreased activities antioxidant enzymes, support notion induction Mechanistically, level shortens half-life GCLC SLC7A11 mRNA, resulting low intracellular GSH, failing detoxify leading cellular which facilitate induction. Collectively, our findings suggest restrains by stabilizing transcripts order avoid induces oxidation phospholipids initiate ferroptosis.
Language: Английский
Citations
14