Effect of Mn(II) and Co(II) on Anti-Candida Metabolite Production by Aspergillus sp. an Endophyte Isolated from Dizygostemon riparius (Plantaginaceae) DOI Creative Commons
Anne Karoline Maiorana Santos,

Bianca Araújo dos Santos,

Josivan Regis Farias

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1678 - 1678

Published: Dec. 12, 2024

Background/Objectives: This study evaluates the effect of Mn(II) and Co(II) ions on production anti-Candida metabolites by endophytic fungus Aspergillus sp., isolated from Dizygostemon riparius. The objective was to identify metal-induced secondary with antifungal potential against drug-resistant Candida species. Methods: sp. cultivated in Czapek agar supplemented MnCl₂ (400 µM) or CoCl₂ (200 µM). Metabolite profiles were analyzed using UHPLC-DAD LC-ESI-HRMS, followed structural elucidation via NMR. Antifungal biofilm inhibition activities tested albicans parapsilosis. Toxicity assessed Tenebrio molitor larvae. Results: Key metabolites, including pyrophen, penicillquei B, fonsecinone demonstrated activity MIC values 4.37–280.61 µg/mL. Fonsecinone B exhibited superior inhibition, surpassing fluconazole reducing biomass viability. In vivo assays showed low toxicity, survival rates above 80% at 2× MIC/kg. Conclusions: significantly modulated emerged as a promising candidate for therapy due its potent toxicity. These findings support further investigation into therapeutic fungal combating infections.

Language: Английский

Recent developments in Aspergillus fumigatus research: diversity, drugs, and disease DOI
Nicole Kordana, Angus Johnson, Karen Quinn

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

SUMMARY Advances in modern medical therapies for many previously intractable human diseases have improved patient outcomes. However, successful disease treatment outcomes are often prevented due to invasive fungal infections caused by the environmental mold Aspergillus fumigatus . As contemporary antifungal not experienced same robust advances as other therapies, defining mechanisms of A. initiation and progression remains a critical research priority. To this end, World Health Organization recently identified priority pathogen Centers Disease Control has highlighted emergence triazole-resistant isolates. The expansion diversity host populations susceptible aspergillosis complex dynamic genotypic phenotypic call reinvigorated assessment pathobiological drug-susceptibility mechanisms. Here, we summarize recent advancements field discuss challenges our understanding heterogeneity its pathogenesis diverse populations.

Language: Английский

Citations

1
Molecular Monitoring of Fungicide Resistance in Crop Phytopathogens DOI Creative Commons
Katherine G. Zulak

Molecular Plant-Microbe Interactions, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

The fight against crop pathogens relies mainly on host genetics and chemistry; however, both areas are compromised by the evolution of resistance in pathogen population. Fungicide is an ongoing challenge to global food security, as it threatens these important protection chemistries. One critical component management effective detection monitoring program, which needs be agile, scalable, sensitive, accurate, cost effective. A rapidly evolving suite molecular tools being developed for fungicide mutations phytopathogen populations, including high-throughput PCR-based quantitative assays cutting-edge third-generation DNA sequencing. single “silver bullet” technology that will satisfy all study objectives does not exist; thus, every tool has a niche integrated program. This review presents overview changing landscape detection, illustrates how techniques exploited combat cereal phytopathogens, highlights challenges future research directions aid design systems aim apply fungicides strategically minimize resistance. [Formula: see text] Copyright © 2025 Author(s). open access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

Citations

0
Ascorbic Acid Enhances the Inhibitory Effect of Theasaponins against Candida albicans DOI Open Access
Yuhong Chen, Ying Gao,

Junfeng Yin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10661 - 10661

Published: Oct. 3, 2024

Candida albicans (C. albicans) is a main cause of hospital-acquired fungal infections. Combination therapy promising as novel anti-C. strategy because its better efficacy. Theasaponins are pentacyclic triterpenes in the Camellia genus with multiple biological activities. Our previous studies prove that theasaponins display inhibitory activity against C. albicans. Ascorbic acid (VC) vitamin found many plants shows potential combination therapy. However, whether VC enhances remains unclear. In this study, checkerboard micro-dilution method was used to assess effect (0–80 mmol/L) on (0–1000 μg/mL). Then, effects (31.25 μg/mL), (80 mmol/L), and μg/mL) + planktonic cells different stages biofilm formation were assessed. Transcriptomic analysis conducted investigate molecular mechanisms. According results, enhanced anti-planktonic anti-biofilm The minimum concentration significantly decreased fungicidal efficiency increased addition VC. remarkably aggravated suppression regard various virulence factors albicans, including adhesion, early formation, mature biofilm, cell surface hydrophobicity, phospholipase activity. Compared or groups, level intracellular reactive oxygen species higher, while levels mitochondrial membrane adenosine triphosphate lower group, suggesting more severe oxidative stress, injury, energy deficiency. revealed predominantly suppressed pathways glycolysis, glycerophospholipid metabolism, glutathione cysteine methionine metabolism. This implied deficiency redox imbalance associated combination. These results has be topical antifungal disinfectant.

Language: Английский

Citations

3
Evaluation of environmental factors related to Aspergillus fumigatus azole resistance in the Netherlands DOI Creative Commons
Massimo Cogliati, Jochem B. Buil, Maria Carmela Esposto

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 177923 - 177923

Published: Dec. 6, 2024

Language: Английский

Citations

1
Discovery of allodiploid hybrid species Aspergillus latus in Japan DOI Open Access
Saho Shibata, Momotaka Uchida, Sayaka Ban

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Allodiploid hybrid species, Aspergillus latus , belonging to section Nidulantes is a of A. spinulosporus and unknown species closely related quadrilineatus subltus . This has often been misidentified as the in such nidulans sublatus or other cryptic not reported Japan well Asia so far. In this study, we screened 23 clinical strains identified isolated from 2012 2023 found seven strains. To characterize strains, conducted comprehensive phenotyping including morphological observation, whole genome sequences phylogenetic analysis based on calmodulin ( CaM ) gene. addition, antifungal susceptibility testing for As result, characters were more similar those compared However, ascospore differed that phylogenetically clustered with Furthermore, showed reduced caspofungin amphotericin B while they susceptible azoles. Our results suggest causative pathogen aspergillosis since 2013.

Language: Английский

Citations

0
Effect of Mn(II) and Co(II) on Anti-Candida Metabolite Production by Aspergillus sp. an Endophyte Isolated from Dizygostemon riparius (Plantaginaceae) DOI Creative Commons
Anne Karoline Maiorana Santos,

Bianca Araújo dos Santos,

Josivan Regis Farias

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1678 - 1678

Published: Dec. 12, 2024

Background/Objectives: This study evaluates the effect of Mn(II) and Co(II) ions on production anti-Candida metabolites by endophytic fungus Aspergillus sp., isolated from Dizygostemon riparius. The objective was to identify metal-induced secondary with antifungal potential against drug-resistant Candida species. Methods: sp. cultivated in Czapek agar supplemented MnCl₂ (400 µM) or CoCl₂ (200 µM). Metabolite profiles were analyzed using UHPLC-DAD LC-ESI-HRMS, followed structural elucidation via NMR. Antifungal biofilm inhibition activities tested albicans parapsilosis. Toxicity assessed Tenebrio molitor larvae. Results: Key metabolites, including pyrophen, penicillquei B, fonsecinone demonstrated activity MIC values 4.37–280.61 µg/mL. Fonsecinone B exhibited superior inhibition, surpassing fluconazole reducing biomass viability. In vivo assays showed low toxicity, survival rates above 80% at 2× MIC/kg. Conclusions: significantly modulated emerged as a promising candidate for therapy due its potent toxicity. These findings support further investigation into therapeutic fungal combating infections.

Language: Английский

Citations

0