Droplet,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 18, 2025
Abstract
Compartmentalization
in
living
systems,
where
multiple
reactions
occur
parallel
within
confined
spaces,
has
inspired
the
development
of
droplet
networks
past
decade.
These
fascinating
assemblies
offer
unique
and
versatile
functions
that
are
unattainable
by
single
droplets
have
shown
their
potential
as
advanced
platforms
for
chemical
biological
applications.
This
review
highlights
recent
progress
creation
application
networks,
covering
strategies
generating
assembling
them
into
functional
networks.
Key
applications
such
microreactors,
signal
conductors,
actuators,
power
sources
summarized.
We
also
discuss
challenges
future
trends
this
field,
aiming
to
narrow
gap
between
fundamental
research
real
Science,
Journal Year:
2023,
Volume and Issue:
382(6672), P. 820 - 828
Published: Nov. 2, 2023
Mitochondria
must
maintain
adequate
amounts
of
metabolites
for
protective
and
biosynthetic
functions.
However,
how
mitochondria
sense
the
abundance
regulate
metabolic
homeostasis
is
not
well
understood.
In
this
work,
we
focused
on
glutathione
(GSH),
a
critical
redox
metabolite
in
mitochondria,
identified
feedback
mechanism
that
controls
its
through
mitochondrial
GSH
transporter,
SLC25A39.
Under
physiological
conditions,
SLC25A39
rapidly
degraded
by
protease
AFG3L2.
Depletion
dissociates
AFG3L2
from
SLC25A39,
causing
compensatory
increase
uptake.
Genetic
proteomic
analyses
putative
iron-sulfur
cluster
matrix-facing
loop
as
essential
regulation,
coupling
iron
to
import.
Altogether,
our
work
revealed
paradigm
autoregulatory
control
organelles.
Proceedings of the National Academy of Sciences,
Journal Year:
2023,
Volume and Issue:
120(20)
Published: May 8, 2023
The
Golgi
is
a
membrane-bound
organelle
that
essential
for
protein
and
lipid
biosynthesis.
It
represents
central
trafficking
hub
sorts
proteins
lipids
to
various
destinations
or
secretion
from
the
cell.
has
emerged
as
docking
platform
cellular
signaling
pathways
including
LRRK2
kinase
whose
deregulation
leads
Parkinson
disease.
dysfunction
associated
with
broad
spectrum
of
diseases
cancer,
neurodegeneration,
cardiovascular
diseases.
To
allow
study
at
high
resolution,
we
report
rapid
immunoprecipitation
technique
(Golgi-IP)
isolate
intact
mini-stacks
subsequent
analysis
their
content.
By
fusing
Golgi-resident
TMEM115
three
tandem
HA
epitopes
(GolgiTAG),
purified
using
Golgi-IP
minimal
contamination
other
compartments.
We
then
established
an
pipeline
liquid
chromatography
coupled
mass
spectrometry
characterize
human
proteome,
metabolome,
lipidome.
Subcellular
proteomics
confirmed
known
identified
not
previously
Golgi.
Metabolite
profiling
metabolome
revealed
enrichment
uridine-diphosphate
(UDP)
sugars
derivatives,
which
consistent
roles
in
glycosylation.
Furthermore,
targeted
metabolomics
validated
SLC35A2
subcellular
transporter
UDP-hexose.
Finally,
lipidomics
showed
phospholipids
phosphatidylcholine,
phosphatidylinositol,
phosphatidylserine
are
most
abundant
glycosphingolipids
enriched
this
compartment.
Altogether,
our
work
establishes
comprehensive
molecular
map
provides
powerful
method
precision
health
Journal of Biological Chemistry,
Journal Year:
2023,
Volume and Issue:
299(12), P. 105457 - 105457
Published: Nov. 9, 2023
One-carbon
metabolism
is
a
central
metabolic
pathway
critical
for
the
biosynthesis
of
several
amino
acids,
methyl
group
donors,
and
nucleotides.
The
mostly
relies
on
transfer
carbon
unit
from
acid
serine,
through
cofactor
folate
(in
its
forms),
to
ultimate
acceptors
that
include
nucleotides
groups
used
methylation
proteins,
RNA,
DNA.
Nucleotides
are
required
DNA
replication,
repair,
gene
expression,
protein
translation,
ribosomal
RNA.
Therefore,
one-carbon
essential
cell
growth
function
in
all
cells,
but
specifically
important
rapidly
proliferating
cells.
regulation
aspect
normal
pathological
pathway,
such
as
cancer,
where
hijacking
these
regulatory
mechanisms
feeds
an
increased
need
regulated
at
levels:
via
posttranslational
modification,
subcellular
compartmentalization,
allosteric
inhibition,
feedback
regulation.
In
this
review,
we
aim
inform
readers
relevant
bring
forward
further
study
metabolism.
review
aims
integrate
two
major
aspects
cancer
metabolism-signaling
downstream
nutrient
sensing
metabolism,
because
while
each
proliferation
cancerous
their
integration
comprehensive
understating
cellular
transformed
cells
can
lead
clinically
insights.
FEBS Letters,
Journal Year:
2024,
Volume and Issue:
598(10), P. 1235 - 1251
Published: Jan. 24, 2024
Our
body
stores
energy
mostly
in
form
of
fatty
acids
(FAs)
lipid
droplets
(LDs).
From
there
the
FAs
can
be
mobilized
and
transferred
to
peroxisomes
mitochondria.
This
transfer
is
dependent
on
close
opposition
LDs
mitochondria
happens
at
membrane
contact
sites.
However,
composition
dynamics
these
sites
not
well
understood,
which
part
due
dependence
metabolic
state
cell
cell-
tissue-type.
Here,
we
summarize
current
knowledge
contacts
between
both
mammals
yeast
Saccharomyces
cerevisiae,
various
are
studied.
We
discuss
possible
functions
site
their
implication
disease.
The EMBO Journal,
Journal Year:
2024,
Volume and Issue:
43(7), P. 1113 - 1134
Published: Feb. 28, 2024
Abstract
Dysregulated
macrophage
responses
and
changes
in
tissue
metabolism
are
hallmarks
of
chronic
inflammation
the
skin.
However,
metabolic
cues
that
direct
support
functions
skin
poorly
understood.
Here,
we
show
during
sterile
inflammation,
epidermis
macrophages
uniquely
depend
on
glycolysis
TCA
cycle,
respectively.
This
compartmentalisation
is
initiated
by
ROS-induced
HIF-1α
stabilization
leading
to
enhanced
epidermis.
The
end-product
glycolysis,
lactate,
then
exported
epithelial
cells
utilized
dermal
induce
their
M2-like
fates
through
NF-κB
pathway
activation.
In
addition,
psoriatic
disorder
also
driven
such
lactate
metabolite-mediated
crosstalk
between
macrophages.
Notably,
small-molecule
inhibitors
transport
this
setting
attenuate
psoriasis
disease
burden,
suppress
fate
acquisition
Our
study
identifies
an
essential
role
for
metabolite
regulating
which
may
be
effectively
targeted
treat
inflammatory
disorders
as
psoriasis.
