Nature-inspired micropatterns

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: Sept. 7, 2023

Language: Английский

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Ionically annealed zwitterionic microgels for bioprinting of cartilaginous constructs

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025004 - 025004

Published: Jan. 4, 2024

Abstract Foreign body response (FBR) is a pervasive problem for biomaterials used in tissue engineering. Zwitterionic hydrogels have emerged as an effective solution to this problem, due their ultra-low fouling properties, which enable them effectively inhibit FBR vivo . However, no versatile zwitterionic bioink that allows high resolution extrusion bioprinting of implants has thus far been reported. In work, we introduce simple, novel method producing microgel bioink, using alginate methacrylate (AlgMA) crosslinker and mechanical fragmentation fabrication method. Photocrosslinked made carboxybetaine acrylamide (CBAA) sulfobetaine (SBMA) are mechanically fragmented through meshes with aperture diameters 50 90 µ m produce bioink. The bioinks both sizes showed excellent rheological properties were high-resolution printing objects overhanging features without requiring support structure or bath. AlgMA dual role, allowing primary photocrosslinking the bulk hydrogel well secondary ionic crosslinking produced microgels, quickly stabilize printed construct calcium bath microporous scaffold. Scaffolds ∼20% porosity, they supported viability chondrogenesis encapsulated human chondrocytes. Finally, meniscus model was bioprinted, demonstrate bioink’s versatility at large, cell-laden constructs stable further vitro culture promote cartilaginous production. This easy scalable strategy direct cell encapsulation scaffold potential biocompatibility nature

Language: Английский

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Lithography-based 3D printing of hydrogels

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Language: Английский

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Confined bioprinting and culture in inflatable bioreactor for the sterile bioproduction of tissues and organs

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 14, 2024

The future of organ and tissue biofabrication strongly relies on 3D bioprinting technologies. However, maintaining sterility remains a critical issue regardless the technology used. This challenge becomes even more pronounced when volume bioprinted objects approaches dimensions. Here, we introduce novel device called Flexible Unique Generator Unit (FUGU), which is unique combination flexible silicone membranes solid components made stainless steel. Alternatively, can also be printed medical-grade polycarbonate. FUGU designed to support micro-extrusion needle insertion removal, internal adjustment, fluid management. was assessed in various environments, ranging from custom-built basic cartesian sophisticated 6-axis robotic arm bioprinters, demonstrating its compatibility, flexibility, universality across different platforms. Sterility assays conducted under infection scenarios highlight FUGU's ability physically protect against contaminations, thereby ensuring integrity constructs. enabled cultivation 14.5 cm

Language: Английский

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Challenges and solutions in soft tissue grafting for receding gums

International Journal of Community Medicine and Public Health, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Soft tissue grafting plays a critical role in addressing periodontal challenges, particularly managing receding gums. It serves both functional and aesthetic purposes, offering solutions for root exposure, sensitivity, compromised oral health. Advances surgical techniques material science have propelled the field forward, providing clinicians with diverse options to achieve optimal outcomes. Autologous grafts remain standard many cases, valued their biocompatibility success rates, though they present limitations such as donor site morbidity limited availability. Innovative materials like nanofibrous scaffolds hydrogels revolutionized engineering. These mimic extracellular matrix (ECM), promoting cell attachment integration while enabling controlled delivery of bioactive agents. The emergence 3D bioprinting has further enhanced precision, allowing patient-specific graft designs that integrate vascular structures improved survival. Decellularized matrices offer biologically compatible alternative, preserving natural architecture reducing immune response risks. Patient-specific factors, including systemic health, genetic predispositions, site-specific anatomy, significantly influence success. Advanced computational tools predictive models now allow personalized treatment strategies, these variables effectively. Technologies bioreactors imaging systems provide real-time insights into performance, bridging gap between research clinical application. Future directions focus on integrating cutting-edge biomaterials regenerative medicine modelling develop smarter, more adaptive solutions. As continues evolve, emphasis lies achieving outcomes balance functionality, aesthetics, patient satisfaction minimizing risks complications. advancements promise reshape landscape therapy, innovative complex challenges.

Language: Английский

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A Fibronectin (FN)‐Silk 3D Cell Culture Model as a Screening Tool for Repurposed Antifibrotic Drug Candidates for Endometriosis

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract This study advances sustainable pharmaceutical research for endometriosis by developing in vitro 3D cell culture models of endometriotic pathophysiology that allow antifibrotic drug candidates to be tested. Fibrosis is a key aspect endometriosis, yet current it remain limited. work aims bridge the translational gap between fibrosis and preclinical testing non‐hormonal candidates. When grown matrix sustainably produced silk protein functionalized with fibronectin‐derived adhesion motif (FN‐silk), endometrial stromal epithelial cells respond transforming growth factor beta‐1 (TGF‐β1) physiological manner as probed at messenger RNA (mRNA) level. For cells, this response TGF‐β1 not observed spheroids, while spheroids behave similarly FN‐silk networks. Pirfenidone, an approved treatment idiopathic pulmonary fibrosis, reverses TGF‐β1‐induced upregulation mRNA transcripts involved fibroblast‐to‐myofibroblast transdifferentiation networks, supporting pirfenidone's potential repurposed therapy. Overall, cultured networks—which are composed produced, fully defined protein—recapitulate fibrotic cellular behavior high fidelity enable testing.

Language: Английский

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3D (Bio) Printing Combined Fiber Fabrication Methods for Tissue Engineering Applications: Possibilities and Limitations

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract Biofabrication is an emerging interdisciplinary field of engineering that aims to develop technologies for applications in tissue and regenerative medicine. A progressing biofabrication technology 3D (bio) printing (3DBP), which allows controlled spatial deposition cell‐laden bioinks a layer‐by‐layer approach fabricate biologically active constructs. Although 3DBP can create some relevant structures, it uses hydrogels, are isotropic nature do not provide sufficient mechanical properties reconstruct many tissues, such as cartilage, bone, skin. Additionally, hydrogels alone replicate the complex hierarchical buildup native extracellular matrix (ECM), contains both gel‐like fibrous components. Replicating tissue's structure mechanically by incorporating fibers would result enhanced biological performance. This possible integrating fiber fabrication techniques. Thus, harnessing strengths each technique eliminating their limitations. will enable hybrid constructs with multiscale hierarchy performance comparable tissue. review highlight attempts combine methods applications. different techniques discussed, showcasing limitations integration 3DBP.

Language: Английский

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Toolbox for creating three-dimensional liver models

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 731, P. 150375 - 150375

Published: July 9, 2024

Language: Английский

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Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 12(2), P. 350 - 381

Published: Nov. 17, 2023

Bioreactors aid in the functional maturation of tissue engineered/bioprinted constructs by mimicking native microenvironment and cues. These functionally matured are suitable for transplantation applications.

Language: Английский

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Immediate Autogenous Bone Transplantation Using a Novel Kinetic Bioactive Screw 3D Design as a Dental Implant

BioMedInformatics, Journal Year: 2023, Volume and Issue: 3(2), P. 299 - 305

Published: April 6, 2023

The restoration of osseous defects is accomplished by bone grafts and substitutes, which are also called biomaterials. Autogenous grafts, derived from the same individual, can retain viability cells, mainly osteoblasts osteoprogenitor stem they do not lead to an immunologic response, known as gold standard for grafts. There both different techniques devices that be used obtain according needs patients, location, size defect. Here, innovative technique presented in patient’s own removed trigone retromolar region mandible inserted into a dental alveolus after extraction immediate insertion implant, BKS. first step creates surgical alveolus; second perforates BKS region, shortly after, containing grafted removed; third screws collects created alveolus. Experimental studies have shown feasibility practicality this new implant model autogenous transplants.

Language: Английский

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