Polymer-Based Hydrogels Applied in Drug Delivery: An Overview

Gels, Journal Year: 2023, Volume and Issue: 9(7), P. 523 - 523

Published: June 27, 2023

Polymer-based hydrogels are hydrophilic polymer networks with crosslinks widely applied for drug delivery applications because of their ability to hold large amounts water and biological fluids control release based on unique physicochemical properties biocompatibility. Current trends in the development hydrogel systems involve drugs response specific triggers such as pH, temperature, or enzymes targeted reduce potential systemic toxicity. In addition, developing injectable formulations that easily used sustain during this extended time is a growing interest. Another emerging trend synthesis nano other functional substances improving loading efficacy. Following these trends, advanced possessing mechanically improved properties, controlled rates, biocompatibility focus field. More complex multi-drug combination therapies will be developed advancements. polymer-based gaining increasing attention personalized medicine tailored patient, example, combinations, target-specific delivery, improvement disease treatment effectiveness, healthcare cost reduction. Overall, application advancing rapidly, towards more efficient effective future.

Language: Английский

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Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

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Ultrasound-assisted tissue engineering

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(6), P. 486 - 500

Published: April 2, 2024

Language: Английский

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Challenges and Future Perspectives in Modeling Neurodegenerative Diseases Using Organ‐on‐a‐Chip Technology

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 23, 2024

Abstract Neurodegenerative diseases (NDDs) affect more than 50 million people worldwide, posing a significant global health challenge as well high socioeconomic burden. With aging constituting one of the main risk factors for some NDDs such Alzheimer's disease (AD) and Parkinson's (PD), this societal toll is expected to rise considering predicted increase in population limited progress development effective therapeutics. To address failure rates clinical trials, legislative changes permitting use alternatives traditional pre‐clinical vivo models are implemented. In regard, microphysiological systems (MPS) organ‐on‐a‐chip (OoC) platforms constitute promising tool, due their ability mimic complex human‐specific tissue niches vitro. This review summarizes current modeling using OoC technology discusses five critical aspects still insufficiently addressed date. Taking these into consideration future MPS will advance vitro translational value setting.

Language: Английский

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Advances in Cell‐Rich Inks for Biofabricating Living Architectures

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(27)

Published: April 19, 2024

Abstract Advancing biofabrication toward manufacturing living constructs with well‐defined architectures and increasingly biologically relevant cell densities is highly desired to mimic the biofunctionality of native human tissues. The formulation tissue‐like, cell‐dense inks for remains, however, challenging at various levels bioprinting process. Promising advances have been made this goal, achieving relatively high that surpass those found in conventional platforms, pushing current boundaries closer tissue‐like densities. On focus, herein overarching challenges bioprocessing cell‐rich into clinically grade engineered tissues are discussed, as well most recent ink formulations their processing technologies highlighted. Additionally, an overview foreseen developments field provided critically discussed.

Language: Английский

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Synthetic and natural polymer hydrogels: A review of 3D spheroids and drug delivery

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 136126 - 136126

Published: Sept. 28, 2024

Language: Английский

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Organoids in concert: engineering in vitro models toward enhanced fidelity

Aggregate, Journal Year: 2024, Volume and Issue: 5(2)

Published: Jan. 4, 2024

Abstract Organoids have emerged as a powerful platform for studying complex biological processes and diseases in vitro. However, most studies focused on individual organoids, overlooking the inter‐organ interactions vivo limiting physiological relevance of models. To address this limitation, development multi‐organoid system has gained considerable attention. This aims to recapitulate communication enable study processes. review provides comprehensive overview recent advancements organoid engineering emerging strategies constructing system. First, we highlight critical mechanical, structural, biochemical factors involved designing suitable materials growth different organoids. Additionally, discuss incorporation dynamic culture environments enhance inter‐organoid communication. Furthermore, explore techniques manipulating morphogenesis spatial positioning organoids establish effective networks. We summarize achievements utilizing vitro, including assembloids microfluidic platforms. Lastly, existing challenges opportunities developing from its technical bottlenecks scalability applications toward human diseases.

Language: Английский

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Using Liver Organoids as Models to Study the Pathobiology of Rare Liver Diseases

Biomedicines, Journal Year: 2024, Volume and Issue: 12(2), P. 446 - 446

Published: Feb. 17, 2024

Liver organoids take advantage of several important features pluripotent stem cells that self-assemble in a three-dimensional culture matrix and reproduce many aspects the complex organization found within their native tissue or organ counterparts. Compared to other 2D 3D vitro models, are widely believed be genetically stable docile structures can programmed virtually recapitulate certain biological, physiological, pathophysiological original tissues organs vitro. Therefore, exploited as effective substitutes miniaturized models for study developmental mechanisms rare liver diseases, drug discovery, accurate evaluation personalized responses, regenerative medicine applications. However, bioengineering currently faces groundbreaking challenges, including need reasonable size, structured organization, vascularization, functional maturity, reproducibility. In this review, we outlined basic methodologies supplements establish summarized recent technological advances experimental biology. Finally, discussed therapeutic applications current limitations.

Language: Английский

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Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(9)

Published: Nov. 22, 2023

Human intestinal organoids (HIOs) derived from pluripotent stem cells or adult cell biopsies represent a powerful platform to study human development, drug testing, and disease modeling in vitro, serve as source for tissue regeneration therapeutic advances vivo. Synthetic hydrogels can be engineered analogs of the extracellular matrix support HIO growth differentiation. These allow tuning mechanical biochemical properties matrix, offering an advantage over biologically such Matrigel. have been used repopulating transplantable grafts vivo delivery injured site. The use synthetic vitro culture is expected significantly increase relevance screening, modeling, applications.

Language: Английский

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Analysis of gene expression dynamics and differential expression in viral infections using generalized linear models and quasi-likelihood methods

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 9, 2024

Introduction Our study undertakes a detailed exploration of gene expression dynamics within human lung organ tissue equivalents (OTEs) in response to Influenza A virus (IAV), Human metapneumovirus (MPV), and Parainfluenza type 3 (PIV3) infections. Through the analysis RNA-Seq data from 19,671 genes, we aim identify differentially expressed genes under various infection conditions, elucidating complexities virus-host interactions. Methods We employ Generalized Linear Models (GLMs) with Quasi-Likelihood (QL) F-tests (GLMQL) introduce novel Magnitude-Altitude Score (MAS) Relaxed (RMAS) algorithms navigate intricate landscape data. This approach facilitates precise identification potential biomarkers, highlighting host’s reliance on innate immune mechanisms. comprehensive methodological framework includes RNA extraction, library preparation, sequencing, Gene Ontology (GO) enrichment interpret biological significance our findings. Results The differential unveils significant changes triggered by IAV, MPV, PIV3 MAS RMAS enable focused revealing consistent activation interferon-stimulated (e.g., IFIT1, IFIT2, IFIT3, OAS1) across all viruses. GO provides deep insights into defense mechanisms viral strategies exploiting host cellular functions. Notably, structures, such as cilium assembly mitochondrial ribosome assembly, indicate strategic shift priorities. precision methodology is validated 92% mean accuracy classifying respiratory infections using multinomial logistic regression, demonstrating superior efficacy over traditional methods. Discussion highlights interplay between expression, underscoring need for targeted therapeutic interventions. stability reliability MAS/RMAS ranking method, even stringent statistical corrections, critical importance adequate sample size biomarker are not only advances understanding but also sets new benchmark paving way development effective diagnostic strategies.

Language: Английский

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