New Advances in Separation Science for Metabolomics: Resolving Chemical Diversity in a Post-Genomic Era

Chemical Reviews, Journal Year: 2013, Volume and Issue: 113(4), P. 2437 - 2468

Published: March 18, 2013

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTNew Advances in Separation Science for Metabolomics: Resolving Chemical Diversity a Post-Genomic EraNaomi L. Kuehnbaum and Philip Britz-McKibbin*View Author Information Department of Chemistry Biology, McMaster University, Hamilton, Canada*Address: ON, L8S 4M1, Canada. Fax: +1-905-522-2509. E-mail: [email protected]Cite this: Chem. Rev. 2013, 113, 4, 2437–2468Publication Date (Web):March 18, 2013Publication History Received4 December 2012Published online18 March 2013Published inissue 10 April 2013https://pubs.acs.org/doi/10.1021/cr300484shttps://doi.org/10.1021/cr300484sreview-articleACS PublicationsCopyright © 2013 American SocietyRequest reuse permissionsArticle Views6508Altmetric-Citations277LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated by Crossref daily. Find more information about citation counts.The Altmetric Attention Score is quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Supporting SUBJECTS:Chromatography,Ions,Liquid chromatography-mass spectrometry,Metabolism,Metabolomics Get e-Alerts

Language: Английский

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Mass spectrometry‐based plant metabolomics: Metabolite responses to abiotic stress

Mass Spectrometry Reviews, Journal Year: 2015, Volume and Issue: 35(5), P. 620 - 649

Published: Jan. 14, 2015

Metabolomics is one omics approach that can be used to acquire comprehensive information on the composition of a metabolite pool provide functional screen cellular state. Studies plant metabolome include analysis wide range chemical species with diverse physical properties, from ionic inorganic compounds biochemically derived hydrophilic carbohydrates, organic and amino acids, hydrophobic lipid‐related compounds. This complexitiy brings huge challenges analytical technologies employed in current metabolomics programs, powerful tools are required for separation characterization this extremely high compound diversity present biological sample matrices. The use mass spectrometry (MS)‐based platforms profile stress‐responsive metabolites allow some plants adapt adverse environmental conditions fundamental biotechnology research programs understanding development stress‐tolerant plants. In review, we describe recent applications emphasize its increasing application study responses (stress‐) factors, including drought, salt, low oxygen caused by waterlogging or flooding soil, temperature, light oxidative stress (or combination them). Advances global changes occurring metabolism under specific abiotic enhance fitness increase tolerance. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 35:620–649, 2016

Language: Английский

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Livestock metabolomics and the livestock metabolome: A systematic review

PLoS ONE, Journal Year: 2017, Volume and Issue: 12(5), P. e0177675 - e0177675

Published: May 22, 2017

Metabolomics uses advanced analytical chemistry techniques to comprehensively measure large numbers of small molecule metabolites in cells, tissues and biofluids. The ability rapidly detect quantify hundreds or even thousands within a single sample is helping scientists paint far more complete picture system-wide metabolism biology. also allowing researchers focus on measuring the end-products complex, hard-to-decipher genetic, epigenetic environmental interactions. As result, metabolomics has become an increasingly popular "omics" approach assist with robust phenotypic characterization humans, crop plants model organisms. Indeed, now routinely used biomedical, nutritional research. It being livestock research monitoring. purpose this systematic review quantitatively objectively summarize current status identify emerging trends, preferred technologies important gaps field. In conducting we critically assessed applications key areas such as animal health assessment, disease diagnosis, bioproduct biomarker discovery for highly desirable economic traits (i.e., feed efficiency, growth potential milk production). A secondary goal critical was compile data known composition metabolome (for 5 most common species namely cattle, sheep, goats, horses pigs). These have been made available through open access, comprehensive database (LMDB, at http://www.lmdb.ca). LMDB should enable producers conduct targeted metabolomic studies where further coverage needed.

Language: Английский

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New materials and trends in sorbents for solid-phase extraction

TrAC Trends in Analytical Chemistry, Journal Year: 2012, Volume and Issue: 43, P. 14 - 23

Published: Nov. 28, 2012

Language: Английский

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Mass spectrometric based approaches in urine metabolomics and biomarker discovery

Mass Spectrometry Reviews, Journal Year: 2015, Volume and Issue: 36(2), P. 115 - 134

Published: April 16, 2015

Urine metabolomics has recently emerged as a prominent field for the discovery of non‐invasive biomarkers that can detect subtle metabolic discrepancies in response to specific disease or therapeutic intervention. Urine, compared other biofluids, is characterized by its ease collection, richness metabolites and ability reflect imbalances all biochemical pathways within body. Following urine collection metabolomic analysis, samples must be immediately frozen quench any biogenic and/or non‐biogenic chemical reactions. According aim experiment; sample preparation vary from simple procedures such filtration more extraction protocols liquid‐liquid extraction. Due lack comprehensive studies on metabolome stability, higher storage temperatures (i.e. 4°C) repetitive freeze‐thaw cycles should avoided. To date, among analytical techniques, mass spectrometry (MS) provides best sensitivity, selectivity identification capabilities analyze majority metabolite composition urine. Combined with qualitative quantitative MS, due continuous improvements related technologies ultra high‐performance liquid chromatography [UPLC] hydrophilic interaction [HILIC]), (LC)‐MS unequivocally most utilized informative tool employed metabolomics. Furthermore, differential isotope tagging techniques provided solution ion suppression matrix thus allowing analysis. In addition LC‐MS, MS‐based have been These include direct injection (infusion)‐MS, capillary electrophoresis‐MS gas chromatography‐MS. this article, current progresses different exploring well recent findings providing potentially diagnostic urinary are discussed. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:115–134, 2017.

Language: Английский

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Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production

Journal of Advanced Research, Journal Year: 2020, Volume and Issue: 24, P. 337 - 352

Published: April 30, 2020

Plants in nature are constantly exposed to a variety of abiotic and biotic stresses which limits their growth production. Enhancing crop yield production feed exponentially growing global population sustainable manner by reduced chemical fertilization agrochemicals will be big challenge. Recently, the targeted application beneficial plant microbiome cocktails counteract stress is gaining momentum becomes an exciting frontier research. Advances next generation sequencing (NGS) platform, gene editing technologies, metagenomics bioinformatics approaches allows us unravel entangled webs interactions holobionts core microbiomes for efficiently deploying increase crops nutrient acquisition resistance stress. In this review, we focused on shaping rhizosphere susceptible host from resistant comprises specific type microbial community with multiple potential benefits CRISPR/Cas9 based strategies manipulation susceptibility genes plants improving health. This review significant providing first-hand information improve fundamental understanding process helps microbiome.

Language: Английский

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Gut Microbial Activity, Implications for Health and Disease: The Potential Role of Metabolite Analysis

Journal of Proteome Research, Journal Year: 2012, Volume and Issue: 11(12), P. 5573 - 5585

Published: Nov. 1, 2012

Microbial metabolism of proteins and amino acids by human gut bacteria generates a variety compounds including phenol, indole, sulfur branched chain fatty acids, many which have been shown to elicit toxic effect on the lumen. Bacterial fermentation occurs mainly in distal colon, site that is often fraught with symptoms from disorders ulcerative colitis (UC) colorectal cancer (CRC). In contrast carbohydrate microbiota, proteolysis less extensively researched. Many metabolites are low molecular weight, volatile compounds. This review will summarize use analytical methods detect identify order elucidate relationship between specific dietary proteinaceous substrates, their corresponding metabolites, implications for gastrointestinal health.

Language: Английский

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Integration of GC–MS and LC–MS for untargeted metabolomics profiling

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2020, Volume and Issue: 190, P. 113509 - 113509

Published: Aug. 2, 2020

Language: Английский

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Serum metabolomics as a novel diagnostic approach for disease: a systematic review

Analytical and Bioanalytical Chemistry, Journal Year: 2012, Volume and Issue: 404(4), P. 1239 - 1245

Published: May 31, 2012

Language: Английский

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Dereplication strategies in natural product research: How many tools and methodologies behind the same concept?

Phytochemistry Reviews, Journal Year: 2015, Volume and Issue: 16(1), P. 55 - 95

Published: Nov. 19, 2015

Language: Английский

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