Impressive stability-indicating RP-HPLC method for concurrent quantification of salbutamol, guaifenesin, and sodium benzoate in cough syrup: Application of six sigma and green metrics

Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 44(1)

Published: Jan. 1, 2025

Abstract Analytical chemistry research has shifted towards environmentally friendly substitutes that reduce waste and avoid hazardous substances. These efforts align with broader environmental goals inspire sustainable responsible science. The objective of this work is to develop validate an analytical method verify its greenness using specific tools, including GREEnness (AGREE), metric for sample preparation (AGREEprep), green procedure index, complex Eco-Scale, volume intensity, score, HPLC-EAT (Environmental Assessment Tool). Effective treatment chronic cough relies heavily on adequately utilizing salbutamol (SAL) guaifenesin (GUA) in the presence sodium benzoate (SOB) preservative. isocratic elution mode used Zorbax Eclipse Plus C18 column (15 cm × 4.6 mm, 3.5 µm). It could move 1.5 mL·min −1 , hold 50 µL, have a temperature 30°C, autosampler 5°C. detect UV light at 276 nm had mobile phase 80:20 v/v buffer solution acetonitrile. We successfully established highly accurate calibration curves three components SAL, GUA, SOB within optimal range 2–50 µg·mL . coefficient correlation ( r ) these was impressive 0.9999 SAL while it 0.9998 SOB. recovery values were 100.15%, 100.47%, 100.92% SOB, respectively. selectivity sensitivity ingredients confirmed according International Council Harmonisation guidelines, as no interference occurred from any components. implemented Six Sigma, resulting precision productivity.

Language: Английский

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A greenness evaluation and environmental aspects of solidified floating organic drop microextraction for metals: A review

Trends in Environmental Analytical Chemistry, Journal Year: 2022, Volume and Issue: 37, P. e00194 - e00194

Published: Dec. 21, 2022

Language: Английский

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Greenness, whiteness, and blueness assessment with spider chart solvents evaluation of HPTLC-densitometric method for quantifying a triple combination anti-Helicobacter pylori therapy

Sustainable Chemistry and Pharmacy, Journal Year: 2023, Volume and Issue: 37, P. 101412 - 101412

Published: Dec. 25, 2023

Language: Английский

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Simultaneous Estimation of Fimasartan potassium trihydrate and Atorvastatin calcium with Greenness Assessment using HPLC and UV Spectrophotometric Methods

Green Analytical Chemistry, Journal Year: 2023, Volume and Issue: 6, P. 100067 - 100067

Published: July 13, 2023

Clinical trials have demonstrated that combining fimasartan potassium trihydrate and atorvastatin calcium produces better results for essential hypertension dyslipidemia with fewer side effects than individual drugs. However, no analytical method is available to accurately determine both drugs' qualitative quantitative amounts when used together. Therefore, we are interested in developing validating three methods simultaneously estimating laboratory-prepared tablets. These include a novel, simple, precise, sensitive, robust HPLC technique, two UV spectrophotometric methods. The reversed-phase Shimpack ODS C18 column as stationary phase an isocratic procedure. A mobile consisting of mixture acetonitrile 0.01 M ammonium acetate buffer, (pH adjusted 3.7 using formic acid) at ratio 70:30 v/v, constant flow rate 1 mL/min chromatograms were recorded 234 nm the analytes. Vierordt's simultaneous equation wavelengths (247 263 nm) estimate derive FIMA ATOR. Meanwhile, first-order derivative zero-crossing points 224nm 261nm concentrations ATOR FIMA. statistical analysis variance test revealed significant differences among regarding accuracy precision. Moreover, developed underwent rigorous validation by ICH Q2R1 guidelines, demonstrating their successful application concurrent drugs bulk tablets prepared within laboratory. To assess ecological impact new technique method, greenness profile was evaluated utilizing Analytical eco scale (AES), National environmental index (NEMI), Green procedure (GAPI), software-based metric (AGREE) tools. This evaluation substantiated method's friendliness solvent usage, chemical substances, energy consumption, waste generation.

Language: Английский

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Magnetic molecularly imprinted polymers and carbon dots molecularly imprinted polymers for green micro-extraction and analysis of pharmaceuticals in a variety of matrices

Microchemical Journal, Journal Year: 2024, Volume and Issue: 205, P. 111235 - 111235

Published: July 18, 2024

Language: Английский

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Sustainable chemometric methods boosted by Latin hypercube technique for quantifying the recently FDA-approved combination of bupivacaine and meloxicam in the presence of bupivacaine carcinogenic impurity: Comprehensive greenness, blueness, and whiteness assessments

Microchemical Journal, Journal Year: 2024, Volume and Issue: 200, P. 110276 - 110276

Published: March 2, 2024

Language: Английский

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A dual methodology employing ion-pair chromatography and built-in UV spectrophotometry for quantifying recently approved combination of mometasone and indacaterol in a novel combined metered dose inhaler: assessing the greenness, carbon footprint, blueness, and whiteness

BMC Chemistry, Journal Year: 2024, Volume and Issue: 18(1)

Published: Aug. 3, 2024

Developing analytical techniques that align with green and sustainable chemistry principles is crucial in today's scientific landscape. This work introduces two innovative approaches for the simultaneous quantification of indacaterol (IND) mometasone (MOM), a recently approved combination therapy chronic obstructive pulmonary disease. These methods-rapid isocratic ion pair chromatography (IPC) UV-visible spectrophotometry-demonstrate improved environmental sustainability, cost-effectiveness, versatility compared to existing techniques. The optimized 4-min IPC method achieved excellent resolution (retention times 2.18 ± 0.1 min IND 3.95 MOM), peak symmetry, sensitivity. It utilizes low-cost mobile phase acetonitrile acidified water containing 0.025% sodium dodecyl sulfate (50:50% v/v), making it suitable laboratories standard chromatographic instruments. spectrophotometric approach offers procedures: first derivative ratio methods. serve as simplified, alternatives resource-limited without access advanced Both feature simplified protocols minimize extraction fractionation steps. Comprehensive validation confirmed outstanding accuracy (98-102%) precision (%2 <). Sustainability assessments using ComplexGAPI, AGREE, carbon footprint, BAGI, RGB12 tools demonstrated enhanced performance spectrophotometry methods greenness scores 0.81 0.85, respectively, surpassing 0.63-0.67 range reported Additionally, they showed lower footprints 0.035 0.022 kg CO2 equivalent emissions per sample, 0.079-0.092 conventional procedures. application novel "blueness" "whiteness" concepts BAGI algorithms further superior 87.5 & 90 blueness 88.1 89.8 whiteness. Successfully applied quantify MOM combined capsules, this provides model eco-friendly pharmaceutical analysis maintains high reliability while improving sustainability metrics.

Language: Английский

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Eco-friendly based HPLC and UV-spectrophotometric methods for simultaneous estimation of Efonidipine hydrochloride Ethanolate and Chlorthalidone in their dosage form

Green Analytical Chemistry, Journal Year: 2024, Volume and Issue: 8, P. 100092 - 100092

Published: Jan. 4, 2024

Objective: Efonidipine hydrochloride Ethanolate and Chlorthalidone combination was approved by CDSCO for Phase III clinical trial. This is utilized to pharmacological therapy that can effectively control hypertension situations. The present research includes an RP-HPLC Simultaneous equation UV methods the determination of drug in tablet dosage form. Furthermore, greenness assessment statistical evaluation developed methods. Methods: HPLC chromatography performed using a gradient technique on reversed-phase C18 column with mobile phase based optimized depending polarity molecules. Unesphere Agela Tech., (250mm x 4.6mm i.d, 5μm,) as stationary Acetonitrile: 0.2 M Potassium dihydrogen orthophosphate pH 4 (68: 32 % v/v) phase. method validated at 220 nm 250 methanol: distilled water (20:80, v/v). tools were used assess ecological impact new spectrophotometric method. Results: detection 220nm elution achieve chromatographic separation over linearity 3.0 - 60.0 μg/mL 10.0 200.0 15.0 50.0 (CLT) (EFD) respectively. value RSD within limit indicated precise, accurate recovery shows there no interference from excipients. Greenness data confirmed method's environmental friendliness terms solvent usage, chemical compounds, energy consumption, trash creation. There spectral or interferences formulation excipients discovered. Conclusion: analytical validation parameters found be per acceptance criteria. In accuracy precision, testing employing analysis variance test did not find any significant differences between two To evaluate whiteness method, reliable health risk associated use essential.

Language: Английский

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Environmentally benign first derivative synchronous spectrofluorimetry for the analysis of two binary mixtures containing duloxetine with avanafil or tadalafil in spiked plasma samples

Luminescence, Journal Year: 2024, Volume and Issue: 39(3)

Published: March 1, 2024

Abstract Antidepressants can cause sexual dysfunction side effects, necessitating the co‐administration of phosphodiesterase type 5 inhibitors. The simultaneous determination these drugs in biological fluids is critical for therapeutic drug monitoring. For first time, two binary mixtures containing duloxetine with either avanafil or tadalafil were estimated utilizing simple green spectrofluorimetric methods without need a previous separation step. study was based on derivative synchronous spectrofluorimetry ethanol using change wavelength difference (∆ λ ) 20 and 25 nm second combinations, respectively. Duloxetine at 297.7 331 their mixture, while determined 290.3 nm, linearity achieved over ranges 0.1–1.5 μg mL −1 both 0.01–0.40 tadalafil, limits detection 0.013, 0.022, 0.004 duloxetine, avanafil, Successful application developed approaches accomplished estimation dosage forms as well human plasma excellent percentage recoveries (96–103.75% plasma), which supports suitability use quality control laboratories pharmacokinetic studies. Moreover, adopted approaches' greenness evidenced by applying three tools.

Language: Английский

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Natural deep eutectic solvent-based QuEChERS followed by magnetic beads ELISA for the analysis of ochratoxin A in cereal samples

Food Control, Journal Year: 2024, Volume and Issue: 161, P. 110384 - 110384

Published: Feb. 15, 2024

Language: Английский

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