Single Biomolecule Imaging by Electrochemiluminescence

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(43), P. 17910 - 17914

Published: Oct. 22, 2021

Herein, a single biomolecule is imaged by electrochemiluminescence (ECL) using Ru(bpy)32+-doped silica/Au nanoparticles (RuDSNs/AuNPs) as the ECL nanoemitters. The emission confined to local surface of RuDSNs leading significant enhancement in intensity. To prove concept, protein molecule at electrode initially visualized as-prepared RuDSN/AuNPs Furthermore, nanoemitter-labeled antibody linked cellular membrane image one cell, without interference current and optical background. success single-biomolecule imaging solves long-lasting task ultrasensitive analysis, which should be able provide more elegant information about biology.

Language: Английский

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Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(21), P. 11769 - 11773

Published: March 12, 2021

Abstract Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of by electrochemiluminescence (ECL) microscopy. By regulating the concentrations luminophore and/or co‐reactant, thickness ECL layer can be controlled to match with spatial location different junctions. At a low concentration luminophore, generation is confined electrode surface, thus revealing only cell–matrix adhesions bottom cells. While high remarkably extended decreasing co‐reactant concentration, allowing sequential and cell–cell near apical surface cells, respectively. This strategy not provides new insights into mechanisms but also promises wide applications microscopy bioimaging.

Language: Английский

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Electrogenerated chemiluminescence detection of single entities

Chemical Science, Journal Year: 2021, Volume and Issue: 12(16), P. 5720 - 5736

Published: Jan. 1, 2021

Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL outstanding tool in analytical sensing. The traditional approach measures averaged electrochemical quantities large ensemble individual entities, including molecules, microstructures ions. However, real system usually heterogeneous, study single entities holds great potential elucidating new truths nature which are out assays or hidden complex systems. We would like review development intensity imaging based entity detection place emphasis on small micro/nanoparticles cells. current challenges for perspectives discussed.

Language: Английский

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Electrochemiluminescence Microscopy

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)

Published: May 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

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A Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance Improvement

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 94(1), P. 336 - 348

Published: Dec. 15, 2021

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTA Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance ImprovementSara RebeccaniSara RebeccaniDepartment of Chemistry "Giacomo Ciamician", University Bologna, Bologna 40127, ItalyMore by Sara RebeccaniView Biography, Alessandra ZanutAlessandra ZanutTandon School Engineering, New York University, Brooklyn, 11201, United StatesMore ZanutView Claudio Ignazio SantoClaudio SantoDepartment SantoView Biographyhttps://orcid.org/0000-0003-4802-1671, Giovanni ValentiGiovanni ValentiDepartment ValentiView Biographyhttps://orcid.org/0000-0002-6223-2072, and Francesco Paolucci*Francesco PaolucciDepartment Italy*Email: [email protected]More PaolucciView Biographyhttps://orcid.org/0000-0003-4614-8740Cite this: Anal. Chem. 2022, 94, 1, 336–348Publication Date (Web):December 15, 2021Publication History Published online15 December 2021Published inissue 11 January 2022https://pubs.acs.org/doi/10.1021/acs.analchem.1c05065https://doi.org/10.1021/acs.analchem.1c05065review-articleACS PublicationsCopyright © 2021 The Authors. American Chemical Society. This publication is licensed under CC-BY 4.0. License Summary*You are free to share (copy redistribute) this article in any medium or format adapt (remix, transform, build upon) the material purpose, even commercially within parameters below:Creative Commons (CC): a Creative license.Attribution (BY): Credit must be given creator.View full license*DisclaimerThis summary highlights only some key features terms actual license. It not license has no legal value. Carefully review before using these materials. Open Access indicated. Learn MoreArticle Views5086Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum text downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (6 MB) Get e-AlertscloseSUBJECTS:Electrochemical cells,Electrodes,Layers,Microscopy,Oxidation e-Alerts

Language: Английский

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Recent advances in electrochemiluminescence immunoassay based on multiple-signal strategy

Current Opinion in Electrochemistry, Journal Year: 2021, Volume and Issue: 28, P. 100725 - 100725

Published: March 7, 2021

Language: Английский

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Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(34), P. 18742 - 18749

Published: June 11, 2021

Mitochondria are the subcellular bioenergetic organelles. The analysis of their morphology and topology is essential to provide useful information on activity metabolism. Herein, we report a label-free shadow electrochemiluminescence (ECL) microscopy based spatial confinement ECL-emitting reactive layer image single living mitochondria deposited electrode surface. ECL mechanism freely-diffusing [Ru(bpy)3 ]2+ dye with sacrificial tri-n-propylamine coreactant restrains light-emitting region micrometric thickness allowing visualize individual remarkable sharp negative optical contrast. imaging approach named "shadow ECL" (SECL) reflects imprint local diffusional hindrance reagents by each mitochondrion. statistical colocalization spots functional revealed classical fluorescent biomarkers, MitoTracker Deep Red endogenous intramitochondrial NADH, validates reported methodology. versatility extreme sensitivity further demonstrated visualizing mitochondria, which remain hardly detectable usual biomarkers. Finally, alleviating problems photobleaching phototoxicity associated conventional methods, SECL should find promising applications in structures.

Language: Английский

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A Close Look at Mechanism, Application, and Opportunities of Electrochemiluminescence Microscopy

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(5), P. 414 - 433

Published: March 6, 2023

Electrochemiluminescence (ECL) is a typical luminescence process triggered by electrochemical reactions. Due to the separated signal types, ECL measurements have some merits of high sensitivity, low background, and simple configuration. Coupled with microscopy setup, (ECLM) has unique characteristics also furnished spatiotemporal resolution. Thus, many applications been created, including nanoscale sensing, mechanism deciphering, transient events single objects, ECLM crossover methods. In this review, we will overview development basic knowledge then profile setup design ECLM. Through understanding these two parts, next probe diverse ECLM, combining inter-relation each other. Finally, outlook discussing expectations further progress technology.

Language: Английский

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Electrochemiluminescence Amplification in Bead-Based Assays Induced by a Freely Diffusing Iridium(III) Complex

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(2), P. 933 - 939

Published: Jan. 26, 2023

Heterogeneous electrochemiluminescence (ECL) assays employing tri-n-propylamine as a co-reactant and tris(2,2′-bipyridine)ruthenium(II) ([Ru(bpy)3]2+) derivative an emissive label are integral to the majority of academic commercial applications ECL sensing. This model system is active research area constitutes basis successfully commercialized bead-based immunoassays. Herein, we propose novel approach enhancement such conventional via incorporation second metal coordination complex, [Ir(sppy)3]3– (where sppy = 5′-sulfo-2-phenylpyridinato-C2,N), experimental system. By microscopy, able map spatial distribution emission at surface bead, from [Ru(bpy)3]2+ labels, solution-phase emission, [Ir(sppy)3]3–. The developed [Ir(sppy)3]3–-mediated elicited significant improvement (70.9-fold 0.9 V 2.9-fold 1.2 vs Ag/AgCl) signal labels immobilized on polystyrene bead. dramatic in signal, particularly low oxidation potentials, has important implications for existing heterogeneous ECL-based by amplifying opening new bioanalytical detection schemes, reducing both electrode passivation deleterious side reactions.

Language: Английский

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Iron Single-Atom Catalyst-Enabled Peroxydisulfate Activation Enhances Cathodic Electrochemiluminescence of Tris(bipyridine)ruthenium(II)

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(28), P. 10762 - 10768

Published: July 8, 2023

The tris(bipyridine)ruthenium(II) (Ru(bpy)32+)–tripropylamine anodic electrochemiluminescence (ECL) system has been widely applied in commercial bioanalysis. However, the presence of amine compounds biological environment results unavoidable interference signals, which hinder further extensive use system. In contrast, cathodic Ru(bpy)32+ ECL can overcome these limitations. Ru(bpy)32+/peroxydisulfate (S2O82–, PDS) extensively employed due to its ability produce a sulfate radical anion (SO4•–) with strong oxidation ability, enhances signal. symmetrical molecular structure PDS makes it challenging be activated and causes low luminescence efficiency. To address this issue, we propose an efficient Ru(bpy)32+-based ternary that uses iron–nitrogen–carbon single-atom catalyst (Fe–N–C SAC) as advanced accelerator. Fe–N–C SAC efficiently activate into reactive oxygen species at lower voltage, significantly boosts emission Ru(bpy)32+. Benefiting from outstanding catalytic activity SAC, successfully established biosensor detects alkaline phosphatase high sensitivity, demonstrating feasibility practical application.

Language: Английский

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