Spiral inertial microfluidics for separation and concentration of phytoplankton DOI Creative Commons
Vitor H. Magalhães, Vânia Pinto, Paulo Sousa

et al.

Algal Research, Journal Year: 2023, Volume and Issue: 76, P. 103317 - 103317

Published: Nov. 1, 2023

Harmful algal blooms (HABs) are a recurring phenomenon along all continents, posing global threat, particularly due to shellfish poisoning and public health, their timely monitoring is vital for safeguarding the blue economy. The ability efficiently isolate species of interest, such as harmful dinoflagellates, key process HABs which complex because typically high diversity marine microalgae communities. This study introduces simple spiral microchannel device using inertial microfluidics separate concentrate based on size. effectively concentrates microalgae, achieving up 5.8-fold fluorescence increase after 3 cycles in isolates target with <6 % cell loss. A continuous enrichment method demonstrated 2.85-fold signal Alexandrium minutum 80 min. proved suitable enhance sensitivity devices designed detect phytoplankton species' early blooms.

Language: Английский

Biomedical Applications of Microfluidic Devices: A Review DOI Creative Commons
Ghazaleh Gharib, İsmail Bütün, Zülâl Muganlı

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 1023 - 1023

Published: Nov. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Language: Английский

Citations

105
Cascaded elasto-inertial separation of malignant tumor cells from untreated malignant pleural and peritoneal effusions DOI Creative Commons

Chen Ni,

Dan Wu, Chen Yao

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(4), P. 697 - 706

Published: Jan. 1, 2024

A cascaded elasto-inertial cell separation device for pretreatment-free, high-recovery-ratio, and high-purity of malignant tumor cells from clinical pleural peritoneal effusions.

Language: Английский

Citations

14
Microfluidic techniques for isolation, formation, and characterization of circulating tumor cells and clusters DOI Creative Commons

Celine Macaraniag,

Qiyue Luan, Jian Zhou

et al.

APL Bioengineering, Journal Year: 2022, Volume and Issue: 6(3)

Published: July 15, 2022

Circulating tumor cell (CTC) clusters that are shed from the primary into bloodstream associated with a poor prognosis, elevated metastatic potential, higher proliferation rate, and distinct molecular features compared to single CTCs. Studying CTC may give us information on differences in genetic profiles, somatic mutations, epigenetic changes circulating cells sites. Microfluidic systems offer means of studying through ability efficiently isolate these rare whole blood patients liquid biopsy. Microfluidics can also be used develop

Language: Английский

Citations

29
Distinct separation of multiple CTCs using inertial focusing phenomena utilizing single-looped spiral microfluidic lab-on-chip DOI
Writtick Pakhira, R. Kumar, Khalid Mohd Ibrahimi

et al.

Chemical Engineering Science, Journal Year: 2023, Volume and Issue: 275, P. 118724 - 118724

Published: April 9, 2023

Language: Английский

Citations

18
Sheathless inertial particle focusing methods within microfluidic devices: a review DOI Creative Commons
Tao Peng, Jun Qiang, Shuai Yuan

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 8, 2024

The ability to manipulate and focus particles within microscale fluidic environments is crucial advancing biological, chemical, medical research. Precise high-throughput particle focusing an essential prerequisite for various applications, including cell counting, biomolecular detection, sample sorting, enhancement of biosensor functionalities. Active sheath-assisted techniques offer accuracy but necessitate the introduction external energy fields or additional sheath flows. In contrast, passive methods exploit inherent fluid dynamics in achieving without actuation. This review analyzes latest developments strategies sheathless inertial focusing, emphasizing elasto-inertial microfluidic from channel structure classifications. These methodologies will serve as pivotal benchmarks broader application technologies biological manipulation. Then, prospects future development are also predicted. paper assist understanding design devices.

Language: Английский

Citations

7
3D-Stacked Multistage Inertial Microfluidic Chip for High-Throughput Enrichment of Circulating Tumor Cells DOI Creative Commons
Xiaohan Xu, Xiwei Huang, Jingjing Sun

et al.

Cyborg and Bionic Systems, Journal Year: 2022, Volume and Issue: 2022

Published: Jan. 1, 2022

Whether for cancer diagnosis or single-cell analysis, it remains a major challenge to isolate the target sample cells from large background cell high-efficiency downstream detection and analysis in an integrated chip. Therefore, this paper, we propose 3D-stacked multistage inertial microfluidic sorting chip high-throughput enrichment of circulating tumor (CTCs) convenient analysis. In chip, first stage is spiral channel with trapezoidal cross-section, which has better separation performance than rectangular cross-section. The second third stages adopt symmetrical square serpentine channels different cross-section widths further reducing outlet flow rate easier can separate 5 μ m 15 particles efficiency 92.37% purity 98.10% at high inlet 1.3 mL/min. Meanwhile, (SW480, A549, Caki-1) massive red blood (RBCs) >80%, >90%, concentration fold ~20. proposed work aimed providing processing system that be easily flowing methods rapid CTC

Language: Английский

Citations

28
Spiral Large-Dimension Microfluidic Channel for Flow-Rate- and Particle-Size-Insensitive Focusing by the Stabilization and Acceleration of Secondary Flow DOI
Shaofei Shen, Lei Zhao,

Hanjie Bai

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(4), P. 1750 - 1758

Published: Jan. 12, 2024

Inertial microfluidics has demonstrated its ability to focus particles in a passive and straightforward manner. However, achieving flow-rate- particle-size-insensitive focusing large-dimension channels with simple design remains challenging. In this study, we developed spiral microfluidic channel achieve inertial focusing. By designing unique "big buffering area" "small the microchannel, observed stabilization acceleration of secondary flow. Our optimized allowed for efficient (>99.9%) 15 μm within wide range flow rates (0.5-4.5 mL/min) during long operation duration (0-60 min). Additionally, achieved effective (>95%) different-sized (7, 10, 15, 30 μm) three types tumor cells (K562, HeLa, MCF-7) near inner wall 1 mm outlet when applying different (1-3 mL/min). Finally, successful 3D cell was an device, positioned at distance 50 from wall. strategy stabilizing accelerating Dean-like through configuration proved be highly that is insensitive rate particle size, particularly channels. Consequently, it shows great potential use hand-operated tools cytometry.

Language: Английский

Citations

4
Stabilizing and Accelerating Secondary Flow in Ultralong Spiral Channel for High-Throughput Cell Manipulation DOI
Shaofei Shen,

Xufang Liu,

Kuohai Fan

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11412 - 11421

Published: July 2, 2024

Efficient cell manipulation is essential for numerous applications in bioanalysis and medical diagnosis. However, the lack of stability strength secondary flow, coupled with narrow range practical throughput, severely restricts diverse applications. Herein, we present an innovative inertial microfluidic device that employs a spiral channel high-throughput manipulation. Our investigation demonstrates regulation Dean-like flow microchannel can be achieved through geometric confinement. Introducing ordered microstructures into ultralong (>90 cm) stabilizes accelerates among different loops. Consequently, effective blood cells within wide throughput (1.73 × 10

Language: Английский

Citations

4
Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges DOI Creative Commons
Mark D. Tarn, Kirsty J. Shaw, Polly B. Foster

et al.

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing supercooled liquid water droplets, altering lifetime and radiative properties clouds having substantial impact on weather climate. However, INPs notoriously difficult model due lack information their global sources, sinks, concentrations, activity, necessitating development new instrumentation for quantifying characterizing in rapid automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups recent years as means performing droplet analysis INPs, enabling measurement hundreds or thousands droplets per experiment at temperatures down homogeneous water. The potential microfluidics extends far beyond this, with an entire toolbox bioanalytical separation detection techniques developed over 30 medical applications. Such methods could easily be adapted biological biogenic INP revolutionize field, example, identification quantification bacteria fungi. Combined miniaturized sampling techniques, we can envisage deployment microfluidic sample-to-answer platforms automated, user-friendly field that would enable greater understanding seasonal activity. Here, review various components such platform incorporate highlight feasibility, challenges, endeavor, from assays separations bioanalysis.

Language: Английский

Citations

0
Design and Performance Analysis of Spiral Microchannels for Efficient Particle Separation Using Inertial Microfluidics DOI Creative Commons
Eda Özyilmaz, Gamze Gediz İliş

Micromachines, Journal Year: 2025, Volume and Issue: 16(3), P. 349 - 349

Published: March 19, 2025

Accurate separation in microfluidic devices is crucial for biomedical applications; however, enhancing their performance remains challenging due to computational and experimental constraints. This study aims optimize by systematically refining spiral microchannel configurations the segregation of circulating tumor cells (CTCs) red blood (RBCs) through detailed variable analysis resource-efficient techniques. The design was developed into six variations, considering loop numbers (2, 3, 4), aspect ratios (2.333, 3.333, 5), radii (5, 6, 7 mm), flow rates (1.5, 2, 3 mL/min), surface roughness levels (0, 0.5, 1 μm), particle sizes (12, 18, 24 μm). Simulations were conducted COMSOL Multiphysics evaluated using Taguchi method determine optimal configuration, reducing set from 216 27 an efficient approach. results identified structure as having ratio four loops, a radius 6–7 mm, rate mL/min, μm, diameter μm. Among parameters, (61.2%) had most significant impact, followed number loops (13.9%) (9.4%). optimized demonstrated high efficiency purity, achieving 97.5% 97.6%, respectively. fabrication process involved 3D-printing channel mold, polydimethylsiloxane (PDMS) casting, validating durability scalability proposed design. integrates simulation results, providing robust framework developing next-generation advancing diagnostic targeted therapeutic applications.

Language: Английский

Citations

0