Biotechnology and Genetic Engineering Reviews,
Journal Year:
2023,
Volume and Issue:
unknown, P. 1 - 14
Published: Feb. 28, 2023
Microorganisms
produce
secondary
metabolites
to
survive
under
stressful
conditions.
The
effect
of
drought
and
heat
stress
on
fungi
isolated
from
Arabian
desert
soil
during
the
hot
(ca
40°C)
cool
10°C)
seasons
was
studied
using
genome
mining
approach.
presence
three
stress-related
genes
(calmodulin,
polyketide
synthase
beta
tubulin)
analyzed
molecularly
specific
primers.
in
compared
their
antimicrobial
(ten
bacterial
or
fungal
pathogens)
anticancer
(liver,
cervical
breast)
properties
production
thermostable
enzymes
(phytase
xylanase).
appeared
be
present
sequence
obtained
summer,
while
none
were
winter.
Appreciable
differences
observed
enzyme
activities,
with
summer
activities
high
winter
low.
antagonistic
A.
niger
relatively
stable
varying,
those
P.
chrysogenum
consistently
higher
than
seemed
correlate
highly
chrysogenum,
had
active
isolates
without
any
genes.
season
deserts
yields
biological
useful
biotechnological
solutions.
Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
12(31)
Published: Sept. 8, 2023
Abstract
Ulcerative
colitis
(UC)
is
a
chronic
inflammatory
illness
affecting
the
colon
and
rectum,
with
current
treatment
methods
being
unable
to
meet
clinical
needs
of
ulcerative
patients.
Although
nanomedicines
are
recognized
as
promising
anti‐inflammatory
medicines,
their
application
limited
by
high
cost
unpredictable
safety
risks.
This
study
reveals
that
two
natural
phytochemicals,
berberine
(BBR)
hesperetin
(HST),
self‐assemble
directly
form
binary
carrier‐free
multi‐functional
spherical
nanoparticles
(BBR‐HST
NPs)
through
noncovalent
bonds
involving
electrostatic
interactions,
π–π
stacking,
hydrogen
bonding.
Because
synergistic
activity,
berberine–hesperetin
exhibit
significantly
better
therapeutic
effects
on
UC
inhibitory
inflammation
than
BBR
HST
at
same
dose
regulating
immune
microenvironment
repairing
damaged
intestinal
barrier.
Furthermore,
BBR‐HST
NPs
good
biocompatibility
biosafety.
Thus,
this
proves
potential
novel
agents
for
UC,
which
could
promote
progress
drug
development
eventually
benefit
patients
who
suffering
from
it.
Plants,
Journal Year:
2024,
Volume and Issue:
13(7), P. 965 - 965
Published: March 27, 2024
In
recent
years,
discovering
new
drug
candidates
has
become
a
top
priority
in
research.
Natural
products
have
proven
to
be
promising
source
for
such
discoveries
as
many
researchers
successfully
isolated
bioactive
compounds
with
various
activities
that
show
potential
candidates.
Among
these
compounds,
phenolic
been
frequently
due
their
biological
activities,
including
role
antioxidants,
making
them
treating
diseases
related
oxidative
stress.
The
isolation
method
is
essential,
and
sought
find
effective
procedures
maximize
the
purity
yield
of
compounds.
This
review
aims
provide
information
on
or
separation
methods
antioxidant
using
column
chromatography,
medium-pressure
liquid
high-performance
counter-current
hydrophilic
interaction
supercritical
fluid
molecularly
imprinted
technologies,
thin
layer
chromatography.
For
purification,
technologies
represent
more
accessible
efficient
procedure
because
they
can
applied
directly
extract
reduce
complicated
process.
However,
it
still
requires
further
development
refinement.
Biotechnology Advances,
Journal Year:
2024,
Volume and Issue:
71, P. 108307 - 108307
Published: Jan. 5, 2024
Bioassays
are
the
main
tool
to
decipher
bioactivities
from
natural
resources
thus
their
selection
and
quality
critical
for
optimal
bioprospecting.
They
used
both
in
early
stages
of
compounds
isolation/purification/identification,
later
evaluate
safety
efficacy.
In
this
review,
we
provide
a
comprehensive
overview
most
common
bioassays
discovery
development
new
bioactive
with
focus
on
marine
bioresources.
We
present
list
practical
considerations
selecting
appropriate
discuss
detail
typically
explore
antimicrobial,
antibiofilm,
cytotoxic,
antiviral,
antioxidant,
anti-ageing
potential.
The
concept
control
bioassay
validation
introduced,
followed
by
considerations,
which
advancing
higher
stage
development.
conclude
providing
an
application-oriented
view
focused
pharmaceuticals,
food
supplements,
cosmetics,
industrial
pipelines
where
currently
known
products
hold
highlight
importance
gaining
reliable
results,
as
these
serve
starting
point
application-based
further
testing,
well
consideration
regulatory
authorities.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(13), P. 5095 - 5105
Published: Feb. 27, 2024
Carbon
dot
(C-dot)
separation/purification
is
not
only
a
fundamental
chemical
issue
but
also
an
essential
precondition
for
revealing
C-dots'
true
nature.
To
date,
adequate
separation
of
C-dots
has
remained
open
question
due
to
the
lack
appropriate
fine
system.
Herein,
we
discover
and
reveal
that
polyamide
chromatography
can
provide
versatile
powerful
performances
C-dot
separation.
By
joint
study
experiments
all-atom
molecular
dynamics
simulations,
demonstrate
multiple
interaction
forces,
including
electrostatic
repulsion/attraction,
hydrogen
bond,
van
der
Waals
effects,
exist
simultaneously
among
stationary
phase,
mobile
separated
C-dots.
Furthermore,
magnitude
these
forces
dependent
on
surface
chemistry
nature
used
phases,
providing
theoretical
basis
experimental
operability
So,
proposed
system
possesses
capacity
adequately
separating
hydrophilic,
amphiphilic,
lipophilic
The
chromatography,
its
performances,
provides
more
thorough
effects
helps
correct
our
false
perceptions
from
inadequate
purified
Natural Product Reports,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
This
review
article
covers
995
natural
products
of
cyanobacterial
origin
reported
from
2010
to
2023.
Compounds
were
analyzed
using
similarity
networking
gain
insights
into
their
chemical
diversity.
