Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 111 - 127
Published: Jan. 1, 2023
Language: Английский
Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)
Published: Jan. 8, 2024
Abstract The COVID-19 outbreak caused by SARS-CoV-2 in late 2019 has spread rapidly across the world to form a global epidemic of respiratory infectious diseases. Increased investigations on diagnostic tools are currently implemented assist rapid identification virus because mass and diagnosis might be best way prevent virus. This critical review discusses detection principles, fabrication techniques, applications with three categories: nuclear acid augmentation test, immunoassay test biosensors. Special efforts were put enhancement nanomaterials biosensors for rapid, sensitive, low-cost diagnostics Future developments suggested regarding potential candidates hospitals, clinics laboratories control prevention large-scale epidemic. Graphical
Language: Английский
Citations
15
Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101188 - 101188
Published: Aug. 7, 2024
Paper based point-of-care (PoC) detection platforms applying lateral flow assays (LFAs) have gained paramount approval in the diagnostic domain as well environmental applications owing to their ease of utility, low cost, and rapid signal readout. It has centralized aspect self-evaluation exhibiting promising potential last global pandemic era Covid-19 implementing management public health remote areas. In this perspective, present review is focused towards landscaping current framework LFAs along with integration components characteristics for improving assay by pushing limits. The highlights synergistic aspects designing, sample enrichment strategies, novel nanomaterials-based transducers, high-end analytical techniques that contribute significantly sensitivity specificity enhancement. Various recent studies are discussed supporting innovations LFA systems focus upon accuracy reliability PoC testing. also provides a comprehensive overview all possible difficulties commercialization subjecting its applicability pathogen surveillance, water food testing, disease diagnostics, agriculture issues.
Language: Английский
Citations
9
Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 68 - 68
Published: Jan. 23, 2025
Multiplex lateral flow assays are one of the greatest advancements in world rapid diagnostics, achieving performance several tests one. These meet basic requirements increasing ease use, low detection limit, and high specificity, as they combine use novel strategies, such exploitation multiple labels, a variety amplification methods. have proven their usefulness many different areas, including clinical food, environmental monitoring. In this review paper, we attempt to highlight discuss predominant changes multianalyte LFAs, related principle, development, combination with other Attention is paid flexibility challenges associated LFA arrays, strategies improve detectability, sensitivity, reliability assays. Therefore, emphasizes current advances field underline possible impact multiplex LFAs on future diagnostics analytical sciences.
Language: Английский
Citations
1
Journal of Immunoassay and Immunochemistry, Journal Year: 2022, Volume and Issue: 43(6), P. 579 - 604
Published: Sept. 15, 2022
Lateral flow immunoassay is the leading Point of Care test and becoming increasingly essential for its versatile properties. The attraction lateral assay (LFA) has reached prime position during recent SARS-CoV-2 pandemic Ebola, Zika epidemics in third world countries where primary screening disease financial issues are very important. During last decade traditional methodology LFA was limited to visual detection qualitative assessment only. However, recently researchers focusing on development improvement this tool enhance specificity, power (quantitative) make it an alternative lab-based technology. Modifying working principle instrumentation, combination different modern molecular techniques such as Reverse transcription loop mediated isothermal amplification (RT-LAMP), Clustered regularly inter-spaced short palindromic repeat (CRISPR-Cas), Recombinase polymerase (RPA), also association image-based software, involvement nanotechnology, implementation ruler have established authenticity ultra-specific level. These immunochromatographic offer simultaneous analytes from a single sample unit into one multiplex strip provide necessary information. This review foremost attempt encompass advances assays with biology along improvements components improved diagnostic sensitivity specificity. Some infectious diagnosis by reporter low limit been mentioned review.
Language: Английский
Citations
15
Microchemical Journal, Journal Year: 2023, Volume and Issue: 193, P. 109159 - 109159
Published: Aug. 1, 2023
Language: Английский
Citations
8
Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 978 - 978
Published: Nov. 7, 2022
The COVID-19 pandemic caused by SARS-CoV-2 has drawn attention to the need for fast and accurate diagnostic testing. Concerns from emerging variants other circulating respiratory viral pathogens further underscore importance of expanding testing multiplex detection, as single-plex may fail detect viruses, while sequencing can be too slow expensive a tool. As result, there have been significant advances in nucleic-acid-based virus testing, creating timely review. This review first introduces frequent nucleic acid targets tests, then proceeds comprehensive up-to-date overview assays that incorporate various detection reactions readout modalities. performances, advantages, disadvantages these are discussed, followed highlights platforms amenable point-of-care use. Finally, this points out remaining technical challenges shares perspectives on future research development. By examining state art synthesizing existing development provide useful resource facilitating ultimately combating COVID-19.
Language: Английский
Citations
10
Biomedical Microdevices, Journal Year: 2023, Volume and Issue: 25(4)
Published: Sept. 8, 2023
Respiratory viruses can cause epidemics or pandemics, which are worldwide outbreaks of disease. The severity these events varies depending on the virus, its characteristics, along with environmental factors. frequency and pandemics caused by respiratory is difficult to predict, but potential such underlines importance continued monitoring, research, preparation for emerging infectious diseases. To help improve pandemic preparedness, we created a fully integrated duplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) device targeting two viruses, influenza A/X-31 virus bovine coronavirus, as replacement SARS-CoV-2. This be adapted any other virus. In this study, showed evaluated prototype microfluidic system, that RT-LAMP detect distinguish between LoDs 2,000 copies/ml coronavirus 200
Language: Английский
Citations
4
Pathogens, Journal Year: 2022, Volume and Issue: 11(11), P. 1252 - 1252
Published: Oct. 28, 2022
This study established a portable and ultrasensitive detection method based on recombinase polymerase amplification (RPA) combined with high-sensitivity multilayer quantum dot (MQD)-based immunochromatographic assay (ICA) to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The RPA-MQD-based ICA is reported for first time has following advantages: (i) RPA free from constraints of instruments can be promoted in point-of-care testing (POCT) scenarios, (ii) fluorescence enhances portability operation so that entire controlled within 1 h, (iii) compared common colorimetric-based RPA-ICA, proposed used MQD provide strong quantifiable signal, thus enhancing sensitivity. With this strategy, amplify SARS-CoV-2 nucleic acid on-site sensitivity copies/reaction, which comparable commercial reverse transcription quantitative chain reaction (RT-qPCR) kits. Moreover, designed primers did not cross-react other viruses, including adenovirus, influenza virus A, B, suggesting high specificity. Thus, sensitively without relying equipment, having good application prospects scenarios under non-lab conditions.
Language: Английский
Citations
7
Analytical Sciences, Journal Year: 2024, Volume and Issue: 40(9), P. 1571 - 1591
Published: May 17, 2024
So far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have advantages rapid detection, simple operation, low cost, ease mass production, no need for special devices professional operators, which make them suitable self-testing at home. This review focuses on SARS-CoV-2 based optical LFAs including colorimetric, fluorescent (FL), chemiluminescent (CL), surface-enhanced Raman scattering (SERS) detection antigens nucleic acids. types recognition components, modes used antigen labels employed in different LFAs, strategies improve sensitivity were reviewed. Meanwhile, coupled with acid amplification techniques CRISPR–Cas systems acids summarized. We hope this provides research mentalities developing highly sensitive that can be home infection.
Language: Английский
Citations
1
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 267, P. 116716 - 116716
Published: Sept. 17, 2024
Language: Английский
Citations
1