Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(6), P. 769 - 784
Published: Nov. 8, 2022
Language: Английский
Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(6), P. 2031 - 2081
Published: Jan. 1, 2023
Bioactive materials are a special class of biomaterials that can react in vivo to induce biological response or regulate functions, thus achieving better curative effect than traditional inert biomaterials. For cancer theranostics, compared with organic polymer nanomaterials, inorganic nanomaterials possess unique physical and chemical properties, have stronger mechanical stability on the basis maintaining certain bioactivity, easy be compounded various carriers (polymer carriers, etc.), so as achieve specific antitumor efficacy. After entering nanoscale, due nano-size effect, high surface area nanostructures, exhibit effects, which significantly influence interaction organisms. Therefore, research applications bioactive theranostics attracted wide attention. In this review, we mainly summarize recent progress also introduce definition, synthesis modification strategies nanomaterials. Thereafter, tumor imaging therapy, including microenvironment (TME) regulation, catalytic gas regulatory cell death immunotherapy, discussed. Finally, biosafety challenges mentioned, their future development opportunities prospected. This review highlights bioapplication
Language: Английский
Citations
132
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(1), P. 206 - 217
Published: Jan. 10, 2023
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTSignal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and PerformanceWei FengWei FengDivision Analytical Environmental Toxicology, Department Laboratory Medicine Pathology, Faculty Dentistry, University Alberta, 10-102 Clinical Sciences Building, Edmonton, Canada T6G 2G3More Wei FengView Biographyhttps://orcid.org/0000-0003-1081-7914, Hongquan ZhangHongquan ZhangDivision ZhangView Biographyhttps://orcid.org/0000-0003-1088-9862, X. Chris Le*X. LeDivision 2G3*[email protected]More LeView Biographyhttps://orcid.org/0000-0002-7690-6701Cite this: Anal. Chem. 2023, 95, 1, 206–217Publication Date (Web):January 10, 2023Publication History Received16 October 2022Published online10 January 2023Published inissue 10 2023https://doi.org/10.1021/acs.analchem.2c04555Copyright © 2023 The Authors. Published American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceCC: Creative CommonsBY: Credit must be given to creatorNC: Only noncommercial uses work are permittedND: No derivatives or adaptations permittedArticle Views712Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (8 MB) Get e-AlertsSUBJECTS:Crystal cleavage,Genetics,Kinetics,Nucleic acids,Peptides proteins e-Alerts
Language: Английский
Citations
62
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 159, P. 116931 - 116931
Published: Jan. 11, 2023
Language: Английский
Citations
43
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(39), P. 26657 - 26666
Published: Aug. 26, 2024
Active clustered regularly interspaced short palindromic repeats (CRISPR/Cas12a) systems possess both
Language: Английский
Citations
19
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(6), P. 2692 - 2701
Published: Feb. 2, 2024
In recent years, the CRISPR/Cas12a-based sensing strategy has shown significant potential for specific target detection due to its rapid and sensitive characteristics. However, "always active" biosensors are often insufficient manipulate nucleic acid with high spatiotemporal control. It remains crucial develop devices that can be activated at desired time space by a remotely applied stimulus. Here, we integrated photoactivation CRISPR/Cas12a system DNA RNA detection, aiming provide control sensing. By rationally designing recognition sequence, this could recognize HPV16 survivin, respectively. We combined lateral flow assay strip test realize visualization of cleavage signals, displaying instant application capabilities. Additionally, also successfully realized temporary fluorescent activity survivin in vivo, allowing acids avoiding risk contamination from premature leaks during storage. Our suggests platform triggered sense various targets, expanding technical toolbox precise biological medical analysis. This study represents advancement applications disease diagnosis treatment.
Language: Английский
Citations
17
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Detection and imaging of dual miRNAs based on AND logic gates can improve the accuracy early diagnosis disease. However, a single target may lead to false positive. Hence, this work rationally integrates hyperbranched rolling circle amplification (HRCA) with Cas12a by replacing PAM sequence bubble sensitively detect image miRNA-10b miRNA-21 gate. When are both present, two padlocks linked into circular DNA as template for RCA. Long ssDNA products generated under catalysis phi29 polymerase, which cis-cleaved activated trans-cleavage generate fluorescent signals. Subsequently, primer hybridizes cis-cleavage is extended dsDNA substrate produce more produces significant signals leading positive due presence protospacer adjacent motif (PAM) padlock. After removed from padlock, RCA form bubbles replace PAM, activate without affecting sensitivity reduce The introduction enables second utilization Cas12a, increasing signal-to-noise ratio. HRCA exhibit optimal activity in T4 ligase buffer, achieving one-pot detection miRNAs. In addition, HRCA-Cas12a method intracellular visualization It exhibits ability distinguish different types cancer cells expression level
Language: Английский
Citations
2
Small, Journal Year: 2023, Volume and Issue: 19(21)
Published: Feb. 25, 2023
Due to their superiority in the simple design and precise targeting, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have attracted significant interest for biosensing. On one hand, CRISPR-Cas capacity precisely recognize cleave specific DNA RNA sequences. other such as orthologs of Cas9, Cas12, Cas13 exhibit cis-cleavage or trans-cleavage activities after recognizing target sequence. Owing cleavage activities, can be designed biosensing by degrading tagged nucleic acids produce detectable signals. To meet requirements point-of-care detection versatile signal readouts, gold nanomaterials with excellent properties high extinction coefficients, easy surface functionalization, biocompatibility are implemented CRISPR-Cas-based biosensors. In combination nanoparticles, nanorods, nanostars, great efforts devoted fabricating biosensors diverse targets. This review focuses on current advances nanomaterials-implemented biosensors, particularly working mechanism performance these systems, including CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a discussed highlighted. Meanwhile, prospects challenges also strategies based systems.
Language: Английский
Citations
40
Food Chemistry, Journal Year: 2023, Volume and Issue: 415, P. 135768 - 135768
Published: Feb. 22, 2023
Language: Английский
Citations
26
Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125663 - 125663
Published: Jan. 16, 2024
Language: Английский
Citations
12
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(26)
Published: Feb. 22, 2024
Abstract Synthetic biochemical circuits (e.g., DNA circuits) remain at the forefront of intracellular biosensing tasks yet are hindered by undesired off‐site activation and accompanying signal leakage. Herein, study attempts to overcome this limitation developing a simple‐yet‐powerful endogenous glutathione (GSH)‐regulating tactic that permits robust distinguishable on‐site microRNA (miRNA) imaging under disturbed redox homeostasis. Specifically, hierarchically activated catalytic (HAD) circuit is fabricated grafting disulfide linkage within entropy‐driven circuitry (EDC) reactants. It exemplified HAD system promises spatiotemporally selective microRNA‐21 (miR‐21) in living cells differentiation tumor from normal cells. The correlationship between GSH miRNA extensively explored live cells, can substantially expand toolbox for profiling processes.
Language: Английский
Citations
10